Estudo das folhas e caule de Hyptidendron canum(Pohl ex Benth.) Harley, Lamiaceae

Abstract

RESUMO:Hyptidendron canum (Pohl ex Benth.) Harley, Lamiaceae, é utilizada popularmente como antimalárica, antiinflamatória, antiulcerativa, anti-hepatotóxica e anticancerígena. O objetivo deste trabalho foi realizar o estudo morfo-anatômico das folhas e caules e identificar as principais classes de metabólitos secundários presentes nas folhas de H. canum, dados ainda não descritos na literatura. As folhas e caules jovens coletados em Goiânia (GO) foram seccionados à mão livre e preparados para análise microscópica. Foram realizadas reações de identificação de metabólitos secundários do material dessecado e pulverizado. Preparou-se o extrato etanólico bruto, que posteriormente foi fracionado por partição líquido-líquido com hexano, clorofórmio e acetato de etila. As frações foram submetidas à análise cromatográfica em camada delgada (CCD). As lâminas foliares apresentam epiderme adaxial constituída por células poligonais com parede reta. Na epiderme abaxial observam-se células com parede reta a ondulada e estômatos diacíticos e anisocíticos. Tricomas tectores e glandulares estão presente em ambas as faces da lâmina foliar. O pecíolo apresenta aspecto canaletado, epiderme adaxial e abaxial unisseriada. O caule, em secção transversal possui contorno em geral quadrangular, com presença de tricomas tectores e glandulares. As reações e a CCD das folhas evidenciaram a presença de flavonóides, saponinas, terpenos e lignanas. Este trabalho contribuiu para um maior conhecimento da morfo-anatomia e das classes químicas presentes em H. canum. Unitermos:Hyptidendron canum, Lamiaceae, morfo-anatomia, CCD. ABSTRACT:Hyptidendron canum (Pohl ex Benth.) Harley, Lamiaceae, is popularly used as an antimalarial, anti-inflammatory, antiulcerative, antihepatotoxic and anticancer agent. The goal of this research was to perform the morphoanatomy study of H. canum leaves and stem and identify the main classes of secondary metabolites present in the of H. canum leaves. Such data have not been reported in the literature. The young leaves and stems were collected in Goiânia (GO), hand sectioned and prepared for microscope analysis. Reactions were performed for the identification of secondary metabolites of the dried and pulverized material. The crude ethanol extract was prepared and then fractioned by liquid-liquid partition with hexane, chloroform and ethyl acetate. Thin layer chromatography (TLC) analysis was performed on the fractions. The leaf blades presented adaxial epidermis constituted of polygonal cells with straight walls. On the abaxial epidermis cells with straight to wavy walls and diacytic and anisocytic stomates were noted. Non-glandular and glandular trichomes are present on both faces of the leaf blade. The petiole is grooved, and it presents single layered adaxial and abaxial epidermis. The cross section of the stem presents a generally quadrangular contour with the presence of non-glandular and glandular trichomes. The leaf reactions and TLC evidenced the presence of flavonoids, saponins, terpenes and lignanes. This works...

Traditional knowledge and uses of medicinal plants by the inhabitants of the islands of the São Francisco river, Brazil and preliminary analysis of Rhaphiodon echinus (Lamiaceae)

“…The concentration of these compounds may vary depending on the solvent of each phase. But these metabolites are always present and can be a mark of this family (Andrade et al, 2010;Fiuza et al, 2010;Galvez et al, 1993).…”

Leaf anatomy and histochemistry of Oriza (Pogostemon cablin Beth., LAMIACEAE): Medicinal plant used in community Arari region, Itacoatiara, Amazonas

“…The nature of the cell wall may also be related to the metabolic activity of the plant. In analyzes performed with the species Melissa officinalis (Lamiaceae) there was an increase in the thickness of the epidermal cells in adaxial and the abaxial were no significant changes(BRANT et al, 2011).Still on the outline of the cell walls and on the distribution of stomata can be compared with the other species also the Lamiaceae family leaves Hyptidendron canum (Pohl x Benth) Harley, whose skin, in front view, had to be different, having hypostomatic sheet and adaxial epidermis in front view, presenting cells with straight anticlinal wall thickened and abaxial epidermis cells with straight wall to wavy, anisocytic and diacytic(FIUZA et al ., 2010). diacytic stomata are more common in the family Lamiaceae (METCALFE, CHALK, 1950), but the anisocítico and anomocítico types can also be found, as recorded in the works ofCASTRO et al, (2015) andDuarte and Lopes (2005), respectively, for ruddy Hyptis species POHL ex Benth and Leonurus sibiricus L LIMA (2010), in a study with other Lamiaceae, Ocimum gratissimum L. pointed out that the leaf of this species proved amphistomatic presenting stomata diacytic, more frequent on the abaxial surface, differing only in the location of stomata on the same level of epidermal cells.…”