Ajuste de metodologias para a identificação de cultivares de soja quanto à tolerância ao glifosato

Pereira, Welison Andrade; Lisboa, Suzana Patrícia; Dias, Denise Cunha Fernandes dos Santos; Alvarenga, Eveline Mantovani; Borém, Aluízio

doi:10.1590/s0101-31222009000400016

Cited by 6 publications

(15 citation statements)

References 12 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…BRSMG 810C, found in the germination test performed with pre-imbibition of seeds in paper towels moistened with the herbicide, was totally inhibited (Table 3); corroborating results obtained by (Tillmann and West, 2004;Cunha et al, 2005;Miranda et al, 2005;and Pereira et al, 2009). As higher the percentage of GM seeds was added in seed samples of non-GM soybean, reductions of normal seedling were detected; indicating the existence of seed mixtures and the feasibility of their detection by the seed analyst.…”

Section: Resultssupporting

confidence: 77%

“…In the literature are also found results on: reduction on length of primary root and its thickening (Funguetto et al, 2004;Pereira et al, 2009); paralyzation of development (Pereira et al, 2009); and lack of secondary roots (Funguetto et al, 2004;Cunha et al, 2005;Pereira et al, 2009). By the analysis of Spearman's correlation, it was observed that there was high and statistically signifi cant correlation between the two methods of detections used (r = 0.82; p ≤ 0.0001).…”

Section: Resultsmentioning

confidence: 97%

“…Variations on length of seedlings were observed, whose mean lengths were 2.9 cm (small seedlings), 6.5 cm (intermediary seedling size), and 9.5 cm (large seedlings); with a total mean length of 6.3 cm. Funguetto et al (2004), andMiranda et al (2005), have also obtained results of bioassays with solutions of glyphosate in which reduction on total length of seedlings occurred; and Pereira et al (2009), have found reduction on occurrence of normal seedlings and secondary roots emission. Table 4.…”

Section: Resultsmentioning

confidence: 97%

“…Some studies, such as those of Miranda et al (2005) and Miranda et al (2006), have assessed the presence of GM soybean seeds in lots of non-GM soybean seeds at ratios of 0%, 1%, 3%, 5% and 8%; while Pereira et al (2009) have found the presence of non-GM soybean seeds in seed lots of GM soybean.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Detection of adventitious presence of genetically modified seeds in lots of non transgenic soybean

Pádua

Madeira²,

Jesus

et al. 2012

Rev. bras. sementes

View full text Add to dashboard Cite

-The difficulty on identifying, lack of segregation systems and absence of suitable standards for coexistence of non trangenic and transgenic soybean are contributing for contaminations that occur during productive system. The objective of this study was to evaluate the efficiency of two methods for detecting mixtures of seeds genetically modified (GM) into samples of non-GM soybean, in a way that seed lots can be assessed within the standards established by seed legislation. Two sizes of soybean samples (200 and 400 seeds), cv. BRSMG 810C (non-GM) and BRSMG 850GRR (GM), were assessed with four contamination levels (addition of GM seeds, for obtaining 0.0%, 0.5%, 1.0%, and 1.5% contamination), and two detection methods: immunoassay of lateral flux (ILF) and bioassay (pre-imbibition into 0.6% herbicide solution; 25 ºC; 16 h). The bioassay is efficient in detecting presence of GM seeds in seed samples of non-GM soybean, even for contamination lower than 1.0%, provided that seeds have high physiological quality. The ILF was positive, detecting the presence of target protein in contaminated samples, indicating test effectiveness. There was significant correlation between the two detection methods (r = 0.82; p ≤ 0.0001). Sample size did not influence efficiency of the two methods in detecting presence of GM seeds.Index terms: Glycine max, glyphosate, transgenic.Detecção de presença adventícia de semente geneticamente modificada em lotes de soja não transgênica RESUMO -A dificuldade de identificação, a falta de sistemas de segregação e a ausência de normas adequadas à coexistência de soja não transgênica e transgênica têm contribuído para que ocorram contaminações durante as etapas do processo produtivo. O objetivo deste trabalho foi verificar a eficiência de dois métodos de detecção de misturas de sementes geneticamente modificadas (GM) em amostras de soja não transgênica, para avaliar lotes de sementes quanto aos limites da legislação. Foram avaliados dois tamanhos de amostra (200 e 400 sementes), das cultivares BRSMG 810C (não transgênica) e BRSMG 850GRR (GM), com quatro níveis de contaminação (sementes GM adicionadas para obter contaminações de 0,0%, 0,5%, 1,0% e 1,5%), e dois métodos de detecção: imunoensaio de fluxo lateral (IFL) e bioensaio (pré-embebição em solução do herbicida a 0,6%; 25 ºC; 16 h). O bioensaio é eficiente na detecção da presença de sementes GM em amostras de semente não transgênica, mesmo para contaminação inferior a 1,0%, desde que apresentem alta qualidade fisiológica. O IFL foi positivo, detectando a presença da proteína alvo nas amostras com contaminação, indicando a eficiência do teste. Houve correlação significativa entre os dois métodos utilizados (r = 0,82; p ≤ 0,0001). O tamanho da amostra não influenciou a eficiência dos dois métodos na detecção da presença de sementes GM.Termos para indexação: Glycine max, glifosato, transgênicos.

show abstract

Section: Resultssupporting

confidence: 77%

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of adventitious presence of genetically modified seeds in lots of non transgenic soybean

Pádua

Madeira²,

Jesus

et al. 2012

Rev. bras. sementes

View full text Add to dashboard Cite

show abstract

“…Plântulas normais foram computadas como resultantes de fluxo gênico. Plântulas anormais, com processo germinativo interrompido, também foram analisadas, pois, é possível caracterizar plântulas anormais tolerantes ao glifosato (Pereira et al, 2009). Plântulas da cultivar sensível ao glifosato caracterizam-se pela ausência de raízes secundárias; sua raiz primária apresenta engrossamento em relação ao seu comprimento (Pereira et al, 2009).…”

Section: Methodsunclassified

Fluxo gênico recíproco entre cultivares de soja convencional e geneticamente modificada

Pereira

Sávio

Dias

et al. 2012

Pesq. agropec. bras.

Self Cite

View full text Add to dashboard Cite

Resumo -O objetivo deste trabalho foi determinar o fluxo gênico recíproco entre duas cultivares de soja, uma tolerante e outra sensível ao glifosato, além de aplicar estimadores para determinar a taxa de fecundação cruzada na população e o número de sementes híbridas na progênie. O experimento compôs-se de quatro blocos com 40 fileiras de soja, com 20 fileiras de cada cultivar (CD217 e CD219RR). No estádio R8, cinco fileiras, distantes 0,5, 1, 2, 4 e 8 m da cultivar adjacente, foram colhidas, trilhadas e analisadas quanto à ocorrência de fluxo gênico. Como características marcadoras, foram utilizadas as cores da flor, hipocótilo e pubescência, e a tolerância ao glifosato. As cultivares contrastam quanto às características analisadas, cada uma condicionada por um gene com dois alelos, em interação de dominância completa. Na progênie da cultivar tolerante, a maior taxa de híbridos encontrada foi 0,27% e, na progênie da cultivar sensível, identificou-se 0,83%; pela hipótese do efeito diluição, as taxas de hibridação natural populacional seriam 0,104 e 0,388%, respectivamente. O fluxo gênico recíproco entre as cultivares CD217 e CD219RR não é o mesmo em ambas as direções. Os estimadores propostos são úteis para determinar a taxa de híbridos em amostras de sementes.Termos para indexação: Glycine max, fecundação cruzada, tolerância ao glifosato, OGM. Reciprocal gene flow between conventional and genetically modified soybean cultivarsAbstract -The objective of this work was to determine the reciprocal gene flow between two soybean cultivars, one tolerant and the other sensitive to glyphosate, as well as to use estimators to determine the outcrossing rate in the population and the number of hybrid seeds in the progeny. The experiment was composed of four blocks of 40 soybean rows, of which 20 rows of each cultivar (CD217 and CD219RR). At the R8 stage, five rows, distant 0.5, 1, 2, 4 and 8 m from the adjacent cultivar, were harvested, threshed and analyzed as for the occurrence of gene flow. As phenotypical markers, the trait color of flowers, hypocotil and pubescence, as well as the tolerance to glyphosate were used. The cultivars contrast for all the analyzed traits, each one conditioned by a single gene with two alleles, in a complete dominance interaction. In the tolerant cultivar progeny, the largest outcross rate was 0.27%, and in the sensitive cultivar progeny, 0.83% was identified; by the dilution effect hypothesis, the outcross rates in natural populations would be 0.104 and 0.388%, respectively. The reciprocal gene flow between CD217 and CD219RR cultivars is not the same in both directions. The proposed estimators are useful for determining the hybrid rates in seed samples.

show abstract

Seed reserve mobilization and seedling morphology in a bioassay for the detection of genetically modified soybean

Costa

Peña

Pereira

2022

Preprint

View full text Add to dashboard Cite

Germination and initial seedling development are physiological processes that determine the establishment of crops. During heterotrophic growth, each seedling develops from the biomass of its seeds. Thus, verifying the potential of genotypes to mobilize reserves under stress has been important. The aim of this study was to investigate how glyphosate affects the mobilization of reserves and seedling morphology. Two tolerant and two herbicide-sensitive cultivars were submitted to germination, seedling length and reserve mobilization tests, including treatments with glyphosate solutions (0, 0.06 and 0.12 %). The hypocotyl / radicle ratio and the efficiency of conversion of reserves to seedlings were also verified. The data were submitted to analysis of variance and test of means. It was observed that the percentage of normal seedlings and length seedlings were affected due to the concentration of the herbicide in the treatments, being the consequences more pronounced for sensitive cultivars; the glyphosate-tolerant genotype and with the best physiological quality mobilized more reserves and was more efficient in converting biomass to seedlings; in morphology, the average length of the seedlings was reduced due to the herbicide, being the roots affected in such a way that they became smaller than the hypocotyls. The herbicide affects the morphology of the seedlings mainly the radicle, and the mobilization of the reserves discriminates the genotypes regarding tolerance to glyphosate.

show abstract

Ajuste de metodologias para a identificação de cultivares de soja quanto à tolerância ao glifosato

Cited by 6 publications

References 12 publications

Detection of adventitious presence of genetically modified seeds in lots of non transgenic soybean

Detection of adventitious presence of genetically modified seeds in lots of non transgenic soybean

Fluxo gênico recíproco entre cultivares de soja convencional e geneticamente modificada

Seed reserve mobilization and seedling morphology in a bioassay for the detection of genetically modified soybean

Contact Info

Product

Resources

About