Influência dos sistemas de pastagem e confinamento na contaminação microbiana de carcaças bovinas

Lemos

et al. 2013

Ars Vet

Escherichia coli O157:H7, an important bacillus strain associated with serious gastroenteritis in humans, is more frequently derived from the consumption of raw or poorly cooked beef. Cattle are important reservoirs suggesting the possibility that feedlot diet management influences the emergence of Shiga-toxigenic strains. This study evaluates the microbiological quality of carcasses and the occurrence of E. coli O157:H7 using the results from general indicator methods (total viable count, coliform rate and E. coli counts) and by an automated PCR method for the detection of E. coli O157:H7. Samples were collected from (industrially processed) meat trimmings and from carcasses of cattle finished on pasture or in feedlots so that sufficient data for the Hazard Analysis and Critical Control Points (HACCP) could be obtained. Samples of rectal swab for experimental detection of E. coli O157:H7 were also collected. One hundred rectal swabs, 100 samples retrieved from warm carcasses and 323 samples of meat trimmings were analyzed. With the exception of one sample of meat trim (0.31%), all the other samples from excreta and carcasses were negative for the O157:H7 E. coli strain. There were no significant differences between the methods used for cattle finishing. Indicator methods results were considered acceptable in 91%, 85% and 93% of tested samples of carcasses respectively for TVC, coliform and E. coli counts. These results agree with statistical data showing the low occurrence of O157:H7 strain.

Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

MICROBIOLOGICAL QUALITY OF CATTLE CARCASS DURING SLAUGHTER AND OCCURRENCE OF E. coli O157: H7 IN BEEF/ Qualidade microbiológica da carcaça bovina durante o processo de abate e a ocorrência de E. coli O157:H7 na ...

Lemos

et al. 2013

Ars Vet

“…Quarenta e cinco (45) amostras de swabs de meias carcaças bovinas, sendo 15 obtidas anteriormente à etapa da lavagem final para resfriamento, 15 obtidos logo após a lavagem final e 15 após 24 horas de permanência em câmara de resfriamento de carcaças. Os locais escolhidos para a obtenção dos swabs nas meias carcaças foram realizados segundo os protocolos descrito por Jardim et al (2006), em que foram demarcadas com moldes estéreis confeccionados de aço inoxidável com área delimitada de 10cm² (um molde para cada ponto). Foram considerados três locais para a coleta das amostras sem meias carcaças bovinas: região do coxão, lombo e ponta de agulha.…”

Section: Methodsunclassified

Caracterização molecular de Listeria monocytogenes oriundas de cortes cárneos bovinos e de abatedouros frigoríficos de bovinos localizados no Distrito Federal, Brasil

et al. 2016

The aim of the study was the analysis of Listeria monocytogenes strains in beef samples as well as slaughterhouse environment, located in the Federal District, promote serotyping by polymerase chain reaction (PCR), perform antibiotic susceptibility and submit the strains to Pulsed-field gel electrophoresis (PFGE). A total of 125 beef samples were analyzed, 45 samples of carcasses swabs and 43 swab samples. It detected 13 strains of Listeria monocytogenes, 11 in beef samples. and 2 in slaughterhouse environment. No carcass swabs strains were isolated. Among the 13 strains of L. monocytogenes six strains of serotype 4b were found, five serotype 1/2c and two strains of serotype 1/2a. Among the 11 strains of L. monocytogenes found in beef, one (9.1%) strain showed resistance to erythromycin, one (9.1%) strain to gentamicin, one to ciprofloxacin (9.1%) and all strains (100%) were resistant to nalidixic acid. The two strains coming from the slaughterhouse drains, all (100%) were resistant to nalidixic acid and Sulfonamides. The analysis by pulsed field gel electrophoresis (PFGE) showed 13 different pulsotypes; they were grouped into three different clonal groups, coincidentally correlated with the three different serotypes found, what suggests a widespread dissemination of these profiles in the Federal District, Brazil.

“…Quarenta e cinco (45) amostras de swabs de meias carcaças bovinas, sendo 15 obtidas anteriormente à etapa da lavagem final para resfriamento, 15 obtidos logo após a lavagem final e 15 após 24 horas de permanência em câmara de resfriamento de carcaças. Os locais escolhidos para a obtenção dos swabs nas meias carcaças foram realizados segundo os protocolos descrito por Jardim et al (2006), em que foram demarcadas com moldes estéreis confeccionados de aço inoxidável com área delimitada de 10 cm² (um molde para cada ponto). Foram considerados três locais para a coleta das amostras sem meias carcaças bovinas: região do coxão, lombo e ponta de agulha.…”

Section: Origem Das Amostrasunclassified

Caracterização molecular de cepas de Listeria monocytogenes oriundas de corte bovino e abatedouros frigoríficos de bovinos localizado no Distrito Federal

Palma¹