O Brasil se destaca na produção mundial de alimentos de origem animal, sendo que cada vez mais os mercados consumidores buscam alimentos seguros e inócuos para a saúde. Nesse contexto, torna-se fundamental a prevenção das zoonoses, que podem ser transmitidas através dos alimentos de origem animal, que muitas vezes são consumidos sem o devido preparo ou por populações mais vulneráveis. Nesta revisão bibliográfica, serão abordadas importantes enfermidades parasitárias de caráter zoonótico que ocorrem atualmente pela via de transmissão alimentar: a toxoplasmose, o complexo teníase-cisticercose, criptosporidiose, anisacose, difilobotriose e triquinelose.
-The acidic ninhydrin spectrophotometric method (ANSM) for quantitative determination of free and bound sialic acid of milk glycoprotein has been proved to be fast and efficient for routine detection of fraudulent addition of rennet whey to fluid milk. In this research the ANSM was compared with the high performance liquid chromatography (HPLC) method, internationally recommended for caseinomacropeptide (CMP) determination, which besides its high accuracy is more sophisticated and requires trained personnel. For several sample conditions (raw milk and milk with variable added amounts of rennet cheese whey), the methods showed an excellent linear correlation, with r = 0.981 when milk was deproteinized with a 120 g·L -1 final concentration of trichloroacetic acid (TCA) concentration. The best correlations could be seen with final concentrations of 100 g·L -1 and 80 g·L -1 TCA; respectively, r = 0.992 and 0.993. Milk / adulteration / whey / addition / acidic ninhydrin / HPLCRésumé -Détection de l'addition frauduleuse de lactosérum au lait de consommation : comparaison de la méthode à la ninhydrine acide (détermination de l'acide sialique) avec la méthode HPLC de détermination du CMP. La méthode spectrophotométrique à la ninhydrine acide (ANSM : acidic ninhydrin spectrophotometric method) pour la détermination des formes libres et liées de l'acide sialique s'est avérée être rapide et performante pour la détection en routine de l'addition frauduleuse de lactosérum doux dans le lait de consommation. Dans l'étude réalisée, l'ANSM a été comparée avec la méthode recommandée au niveau international, à savoir la méthode de détermination du caséinomacropeptide (CMP) par HPLC qui est certes très précise mais requiert un équipement sophistiqué et du personnel hautement qualifié. Une excellente corrélation linéaire a été trouvée entre les deux méthodes lors de l'analyse de filtrats TCA (acide trichloroacétique) issus d'échantillons de lait cru additionnés de quantités variables de lactosérum doux soumis préalable-ment à un traitement thermique. Selon la concentration finale en TCA : 120 g·L -1 , 100 g·L -1 , 80 g·L -1 , les valeurs de corrélation linéaire atteignaient respectivement 0,981, 0,992 et 0,993.Lait / fraude / lactosérum / addition / ninhydrine acide / HPLC * Corresponding author: salvador@fea.unicamp.br 502 S.P. Fukuda et al.
ResumoO complexo teniose-cisticercose bovina configura uma zoonose parasitária conhecida há muito tempo e característica de países subdesenvolvidos. Com importância em saúde pública, é motivo de prejuízos econômicos à cadeia produtiva da carne bovina no Brasil e, na atualidade, sinônimo de inadequação técnica em relação à adoção de Boas Práticas de Produção. As ocorrências, tanto da teniose humana quanto da cisticercose bovina, poderiam e deveriam ser controladas com medidas sanitárias básicas. Entretanto, há muita variação na ocorrência da doença no rebanho bovino, caracterizando condição de baixo índice de desenvolvimento técnico, além de problemas com medidas de saneamento básico. Esta revisão objetivou descrever, em detalhes, o agente etiológico e a cadeia epidemiológica, além de levantar informações atuais sobre sua ocorrência nas diferentes regiões do Brasil, visando à adoção de medidas profiláticas pelos diferentes segmentos responsáveis. Palavras-chave: Bovinos, cisticerco, epidemiologia, Taenia saginata AbstractThe taeniasis-cysticercosis complex is a long known zoonotic parasitosis characteristic of underdeveloped countries. In addition to its public health significance, this parasitosis is cause of economic losses to the beef production chain, and synonymous of technical inadequacy in relation to the adoption of Good Agricultural Practices. The occurrences of both human teniasis and bovine cysticercosis could and should be controlled with basic sanitary measures. However, there is much variation in the occurrence of the disease in cattle, characterizing a low rate of technical development as well as problems related to the adoption of basic sanitation measures. This review describes, in details, the causative agent and its epidemiological chain, besides raising current information about the occurrence of bovine cysticercosis in different regions of Brazil, aiming at the adoption of prophylactic measures by different segments responsible.
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