Incidência de Sclerotinia sclerotiorum em sementes de soja e sensibilidade dos testes de detecção

Henneberg, Luciane; Grabicoski, Edilaine Maurícia Gelinski; Jaccoud-Filho, David de Souza; Panobianco, Maristela

doi:10.1590/s0100-204x2012000600005

Cited by 15 publications

(15 citation statements)

References 7 publications

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“…Similar results were observed by other authors, however without differentiation of the infection level (Napoleão et al, 2006;Henneberg et al, 2012;Botelho et al, 2013). The method ability to acknowledge the pathogen's presence in seeds low percentages of infection, is on its own, relevant.…”

Section: Testing the Presence -Absence Hypothesis Of Pathogen In The Lotsupporting

confidence: 90%

“…Fungi recovered from IAC-Foscarin cultivar C1 soybean seeds are coincident to those reported by Braccini et al (2000), Machado (2000), Goulart (2005), Henning (2009), Henneberg et al (2012), Sousa et al (2011), Barros and Juliatti (2012) as the most common gender in soybean seeds. These species infect the seeds internally or externally, being responsible in most cases for poor seed germination and, as a consequence, the soybean production reduced physiological quality, emphasizing however that the pathogens' incidence transmitted by these seeds are within tolerable levels.…”

Section: Determination Of the Lot Heterogeneitysupporting

confidence: 86%

“…Pérola, "saved seeds") and by the Towel paper method (0,25%, samples 19 and 26 cv. Pérola, "saved seeds") is according to those found by Hoffman et al (1998) Henneberg et al (2012), which means, low levels of the pathogen recovery (0,25 to 2%) by the detection traditional methods.…”

Section: Testing the Presence -Absence Hypothesis Of Pathogen In The Lotmentioning

confidence: 99%

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Sequential sampling of soybean and beans seeds for Sclerotinia sclerotiorum detection (Lib.) DeBary

Ballaris

Machado²,

Carvalho³

et al. 2014

J. Seed Sci.

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ABSTRACT-This study aimed to verify the efficiency of a sequential sampling plan for bean and soybean seeds, in the Sclerotinia sclerotiorum detection. Firstly the heterogeneity of Pérola and Bolinha Bean cultivars lots samples and IACFoscarin soybean was assessed, through H and R testing values, verifying their physiological and sanitary quality. To test the pathogen presence or absence a positive binomial distribution was used, in naturally infected seeds, and recovered by Neon-S and Roll Paper tests. The Neon-S test efficiency was obtained at different levels of infection. Statistical analysis for each test was made in DIC with four replications and the data subjected to variance analysis, and averages compared using the Tukey test (P ≤ 0.05) probability. The soybean and bean seeds sampling sequence for mycelial Sclerotinia sclerotiorum detection was efficient, once at least 800 and 1.000 seeds are evaluated considering 0.01 and 0.005%, incidences respectively. The Neon-S and Towel Paper methods were sensitive to the Sclerotinia sclerotiorum mycelial detection in soybean and bean seeds.Index terms: white mold, mycelial transmission, Neon-S.Amostragem sequencial de sementes de soja e feijão na detecção de Sclerotinia sclerotiorum (Lib.) DeBary RESUMO -O presente trabalho teve por objetivo verificar a eficiência de um plano de amostragem sequencial para sementes de feijão e da soja, na detecção de S. sclerotiorum. Deste modo, primeiramente foi avaliada a heterogeneidade das amostras dos lotes para as cultivares Pérola e Bolinha de feijão e IAC-Foscarin de soja, pelos testes dos valores H e R e, verificadas a sua qualidade fisiológica e sanitária. Para testar a presença ou ausência do patógeno foi utilizada a distribuição binomial positiva, em sementes infectadas naturalmente e recuperadas pelos testes Neon-S e Rolo de Papel. A eficiência do teste Neon-S foi obtida para diferentes níveis de infecção. A análise estatística para cada teste foi feita em DIC, com quatro repetições e, os dados submetidos à análise de variância, sendo as médias comparadas pelo teste de Tukey (P ≤ 0,05) de probabilidade. O plano de amostragem sequencial de sementes de soja e de feijão para detecção de Sclerotinia sclerotiorum na forma micelial foi eficiente, desde que 800 e 1.000 sementes sejam avaliadas, considerando incidências de 0,01 e 0,005%, respectivamente. Os métodos Neon-S e Rolo de Papel foram sensíveis na detecção micelial de Sclerotinia sclerotiorum em sementes de soja e de feijão.Termos para indexação: mofo-branco, transmissão micelial, neon-S.

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Section: Testing the Presence -Absence Hypothesis Of Pathogen In The Lotsupporting

confidence: 90%

Section: Determination Of the Lot Heterogeneitysupporting

confidence: 86%

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Sequential sampling of soybean and beans seeds for Sclerotinia sclerotiorum detection (Lib.) DeBary

Ballaris

Machado²,

Carvalho³

et al. 2014

J. Seed Sci.

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show abstract

“…O patógeno se associa às sementes como micélio dormente ou na forma de escleródios e reduz drasticamente o potencial germinativo, o vigor e a emergência (14).…”

Section: Abstract: Biological Control Glycine Max White Mold Scannunclassified

Emergência e análise ultraestrutural de plântulas de soja inoculadas com Sclerotinia sclerotiorum sob efeito da aplicação de Trichoderma harzianum

et al. 2017

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A elevada susceptibilidade da soja ao mofo-branco tem impactado negativamente a sua produtividade. Atualmente métodos de controle biológico como o uso de Trichoderma spp. têm proporcionado alternativas ao controle químico, com menor impacto ambiental e favorecimento do desenvolvimento vegetal. Objetivou-se avaliar os efeitos de T. harzianum sobre a emergência de sementes de soja inoculadas com Sclerotinia sclerotiorum; além de verificar a interação entre os fungos e com a soja, por meio da microscopia eletrônica de varredura (MEV ABSTRACTThe high susceptibility of soybean to white mold has negatively impacted its productivity. Currently, biological control methods, such as the use of Trichoderma spp., have provided alternatives to chemical control, as they promote less environmental impact and favor the plant growth. The aim of this study was to evaluate the effects of T. harzianum on the emergence of soybean seeds inoculated with Sclerotinia sclerotiorum, besides identifying the interaction between fungi and soybeans by means of scanning electron microscopy (SEM). Soybean seeds were subjected to the following treatments: 1. Without S. sclerotiorum, in PDA+Mannitol; 2. With S. sclerotiorum, in PDA+Mannitol; 3. With Ecotrich ® WP and without S. sclerotiorum, in PDA+Mannitol; 4. Silva, F.F.; Castro, E.M.; Moreira, S.I.; Ferreira, T.C.; Lima, A.E.; Alves, E. Emergence and ultrastructural analysis of soybean seedlings inoculated with Sclerotinia sclerotiorum under the effect of Trichoderma harzianum application. Summa Phytopathologica, v.43, n.1, p.41-45, 2017. With S. sclerotiorum and Ecotrich ® WP, in PDA+Mannitol. The emergence test was conducted on trays with sterile sand at 25 °C for 8 days. Then, the vegetative organs of emerged seedlings were sectioned to evaluate, by scanning electron microscopy (SEM), T. harzianum potential to parasitize and inhibit S. sclerotiorum. In fact, the white mold is capable of colonizing and deteriorating all soybean seeds. T. harzianum is effective in colonizing the root system of soybeans but does not contribute to emergence, compared to control. Based on the ultrastructural analysis, mycoparasitism of T. harzianum and S. Sclerotiorum was evidenced, but the control of the causal agent of white mold was not as effective as shown in other studies.

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“…In the work of Grabicoski (2012), the sampling was carried out in experimental fields, in the conditions of the region of the state of Paraná. Henneberg et al (2012), aiming to evaluate the efficiency of different methods to detect S. sclerotiorum on soybean seeds, carried out a research comparing five methods: the blotter test under three temperatures (7, 14 and 20ºC); germination paper test; and agar-brome-phenol medium (Neon-S). Regardless of the method, there was no relation between the percentage of pathogen incidence and its detection on seeds.…”

Section: Resultsmentioning

confidence: 99%

Detection of Sclerotinia sclerotiorum on soybean and common bean seeds by modified neon-s test

Santos¹,

Morais²,

Juliatti³

2018

Biosci. J.

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ABSTRACT:The Neon-S method has been used for detection of Sclerotinia sclerotiorum on soybean and common bean seeds since the 2010 crop season. However, this method can lead to identification of false-positives due to the presence of other fungi that change the medium pH. Thus, this study evaluated the effect of increasing incubation period on the reliability of Neon-S test in detecting S. sclerotiorum infection on soybean and common bean seeds. A randomized block design was set up with three replicates in a 3x3 factorial scheme, consisting of three detection methods (germination paper test, Neon-S, and modified Neon-S2) and three seed material (naturally infected common beans, naturally infected and artificially inoculated soybean seeds). The three methods were compared by evaluating 400 seeds per replication, after incubating them for seven days in Neon-S, for 15 days in Neon-S2, and for 30 days in germination paper, determining the presence of the fungus and of sclerotia adhered to the seeds. The data were submitted to the analysis of variance and the averages compared by the Tukey test at 5% probability. From 2008 to 2012, 637 lots were evaluated. Among the seed material, artificially inoculated soybean presented the greatest pathogen infection index. The germination paper test led to 2.8% of positive samples, contrasting 29.7% of Neon-S. The modified method Neon-S2 increased detection sensitivity of S. sclerotiorum in seed lots (31.2%); however, did not significantly differ from the Neon-S method, despite its greater averages. We concluded that detection of S. sclerotiorum by the Neon-S method can be optimized by incubation for 15 days (Neon-S2), due to the formation of sclerotia near the infect seeds which confirms the presence of the pathogen avoiding false-positive results.

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Incidência de Sclerotinia sclerotiorum em sementes de soja e sensibilidade dos testes de detecção

Cited by 15 publications

References 7 publications

Sequential sampling of soybean and beans seeds for Sclerotinia sclerotiorum detection (Lib.) DeBary

Sequential sampling of soybean and beans seeds for Sclerotinia sclerotiorum detection (Lib.) DeBary

Emergência e análise ultraestrutural de plântulas de soja inoculadas com Sclerotinia sclerotiorum sob efeito da aplicação de Trichoderma harzianum

Detection of Sclerotinia sclerotiorum on soybean and common bean seeds by modified neon-s test

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