Effect of arcelin protein on the biology of Zabrotes subfasciatus (Boheman 1833), in dry beans

Barbosa, Flávia Rabelo; Yokoyama, Massaru; Pereira, P. A. A.; Zimmermann, F. J. P.

doi:10.1590/s0100-204x1999001000006

Cited by 11 publications

(3 citation statements)

References 8 publications

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“…Arcelin is found in resistant wild varieties but is absent in many susceptible commercial and wild varieties (Romero Andreas et al 1986;Cardona et al 1990;Blair et al 2010;Zaugg et al 2013). Based on previous studies, arcelin, found in several wild genotypes, is associated with resistance in common beans (Osborn et al 1986(Osborn et al , 1988Romero Andreas et al 1986;Cardona et al 1989Cardona et al , 1990Minney et al 1990;Velten et al 2007), and types 1, 2, and 5 are the most resistant against bruchids (Barbosa et al 1999;Baldin and Lara 2008;Sakthivelkumar et al 2013). Arcelin prolongs immature development of A. obtectus by 15% (Schmale et al 2002) and acts as a growth inhibitor on first instar larvae.…”

Section: Introductionmentioning

confidence: 99%

Characterization of resistance to the bean weevil Acanthoscelides obtectus Say, 1831 (Coleoptera: Bruchidae) in common bean genotypes

Baldin

Lara

Camargo

et al. 2017

Arthropod-Plant Interactions

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The bean weevil Acanthoscelides obtectus (Say, 1831) (Coleoptera: Bruchidae) is one of the most serious pests of stored beans worldwide because of the damage it causes to grains within warehouses. The use of resistant genotypes may offer a control strategy for this pest. In the current study, we screened common bean genotypes of Andean American and Mesoamerican origin in laboratory and greenhouse bioassays to select the most promising beans for resistance to the bean weevil. In the laboratory, we evaluated number of eggs, period of development (eggadult), number of emerged adults, dry weight of adults, and weight of consumed grains. In the greenhouse, number of pods per plant and number of grains per pod were evaluated. We also assessed the percentages of damaged pods per plant and damaged grains per pod. Combining the results obtained in the laboratory and greenhouse assays, the common bean genotypes Arc.1, Arc.2, Arc.1S, Arc.5S, and Arc.3S were identified as resistance expressing antibiosis against A. obtectus. The lowest percentages of damaged pods were found in the Arc.1 and Arc.1S genotypes, and their resistance to damage was apparently morphological (antixenotic) because they possessed structures that prevented contact between larvae and grains. The use of resistant genotypes in combination with other techniques may improve management of the weevil. Additionally, the resistant genotypes identified here can be used in breeding programs to develop common bean lines with resistance to A. obtectus.

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Section: Introductionmentioning

confidence: 99%

Characterization of resistance to the bean weevil Acanthoscelides obtectus Say, 1831 (Coleoptera: Bruchidae) in common bean genotypes

Baldin

Lara

Camargo

et al. 2017

Arthropod-Plant Interactions

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“…One of the most famous and important defense proteins is arcelin due to its insecticidal properties, and in particular, because of its inhibitory activity on larval development of pests of stored grains (Cardona et al, 1990;Janarthanan and Suresh, 2003;Janarthanan et al, 2008). Resistance in bean genotypes to weevils caused by the presence of arcelin has been found by several authors (Osborn et al, 1988;Wanderley et al, 1997;Barbosa et al, 1999;Miranda et al, 2002;Mazzonetto and Vendramim, 2002). Another important group of defense proteins that has been reported as the cause of antibiosis resistance to weevils are trypsin inhibitors and vicilin globulins (Gatehouse et al, 1979;Macedo et al, 1993;Sales et al, 2005).…”

Section: Introductionmentioning

confidence: 98%

Antibiosis levels of common bean genotypes toward Zabrotes subfasciatus (Boheman) (Coleoptera: Bruchidae) and its correlation with flavonoids

Eduardo

Júnior

Moraes

et al. 2016

Journal of Stored Products Research

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a b s t r a c tExpression of the antibiosis-resistance category to weevils has been evaluated in several bean genotypes with very promising results. Among the several causes responsible for this resistance category the arcelin protein and trypsin inhibitors stand out. Other mechanisms may be associated with plant resistance to the attack of weevils; however, few studies seek to discover these possible causes. Thus, our research aimed at identifying bean genotypes resistant to Zabrotes subfasciatus, classify them into resistance levels, quantify the content of flavonoids, and correlate it with data obtained from the genotypes. An antibiosis test was performed with beans of 43 genotypes and 40 replications (bean grains) under a completely randomized design. The biological parameters recorded from Z. subfasciatus were the periods from egg to larvae, larvae to adult, egg to adult, longevity and life cycle, in addition to egg viability, adults emerged, susceptibility relative index, adult weight, sex ratio, and the dry mass consumed by larvae. The chemical profile of flavonoids from each genotype was assessed by means of LC-MS. Based on the results of the weevil biological parameters and dry mass consumed provided by univariate and multivariate statistical analyses, the bean genotypes were classified into four levels of resistance (antibiosis): highly resistant, moderately resistant, susceptible and highly susceptible. Among the evaluated flavonoids, we could identify isoquercitrin; however, it has no correlation with the expression of resistance. From the results obtained in this work, more studies will be conducted with the genotypes that stood out as resistant, evaluating other resistance categories and defense mechanisms of these materials against the attack of Z. subfasciatus and other pests, as well as studies of other important agronomic characteristics aiming at future commercialization of the bean genotypes.

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“…So far, six arcelin variants have been isolated and biochemically characterized (Osborn et al, 1986;Lioi & Bollini, 1989;Santino et al, 1991). The main target insect for arcelin is Zabrotes subfasciatus (Barbosa et al, 1999). In contrast, effects of arcelin against A. obtectus are less complete.…”

Section: Introductionmentioning

confidence: 99%

Resistance of stored bean varieties to Acanthoscelides obtectus (Coleoptera: Bruchidae)

Baldin

Lara

2008

Insect Science

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During bean seed storage, yield can be lost due to infestations of Acanthoscelides obtectus Say, the bean weevil. The use of resistant varieties has shown promising results in fighting these insects, reducing infestation levels and eliminating chemical residues from the beans. The expression of resistance to A. obtectus in bean varieties is frequently attributed to the presence of phytohemagglutinins, protease inhibitors and alpha-amylase, and especially to variants of the protein arcelin, which reduce the larval viability of these insects. To evaluate the effect of bean seed storage time on the resistance expression of bean varieties to A. obtectus, tests with seeds of three ages (freshly-harvested, 4-month-old, and 8-monthold) were conducted in the laboratory, using four commercial varieties: Carioca Pitoco, Ipa 6, Porrillo 70, Ônix; four improved varieties containing arcelin protein: Arc.1, Arc.2, Arc. 3, Arc.4; and three wild varieties also containing arcelin protein: Arc.1S, Arc.3S, and Arc. 5S. The Arc.5S, Arc.1S, and Arc.2 varieties expressed high antibiosis levels against the weevil; Arc.1 and Arc3S expressed the same mechanism, but at lower levels. The occurrence of oviposition non-preference was also observed in Arc.5S and Arc.1S. The Arc.3 and Arc. 4 varieties expressed low feeding non-preference levels against A. obtectus. The expression of resistance in arcelin-bearing, wild or improved varieties was affected during the storage of seeds, and was high under some parameters but low in others. The results showed that addition of chemical resistance factors such as protein arcelin via genetic breeding may be beneficial in improving the performance of bean crops.

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Effect of arcelin protein on the biology of Zabrotes subfasciatus (Boheman 1833), in dry beans

Cited by 11 publications

References 8 publications

Characterization of resistance to the bean weevil Acanthoscelides obtectus Say, 1831 (Coleoptera: Bruchidae) in common bean genotypes

Characterization of resistance to the bean weevil Acanthoscelides obtectus Say, 1831 (Coleoptera: Bruchidae) in common bean genotypes

Antibiosis levels of common bean genotypes toward Zabrotes subfasciatus (Boheman) (Coleoptera: Bruchidae) and its correlation with flavonoids

Resistance of stored bean varieties to Acanthoscelides obtectus (Coleoptera: Bruchidae)

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