Characterization of resistance to the bean weevil Acanthoscelides obtectus Say, 1831 (Coleoptera: Bruchidae) in common bean genotypes

Abstract

The bean weevil Acanthoscelides obtectus (Say, 1831) (Coleoptera: Bruchidae) is one of the most serious pests of stored beans worldwide because of the damage it causes to grains within warehouses. The use of resistant genotypes may offer a control strategy for this pest. In the current study, we screened common bean genotypes of Andean American and Mesoamerican origin in laboratory and greenhouse bioassays to select the most promising beans for resistance to the bean weevil. In the laboratory, we evaluated number of eggs, period of development (eggadult), number of emerged adults, dry weight of adults, and weight of consumed grains. In the greenhouse, number of pods per plant and number of grains per pod were evaluated. We also assessed the percentages of damaged pods per plant and damaged grains per pod. Combining the results obtained in the laboratory and greenhouse assays, the common bean genotypes Arc.1, Arc.2, Arc.1S, Arc.5S, and Arc.3S were identified as resistance expressing antibiosis against A. obtectus. The lowest percentages of damaged pods were found in the Arc.1 and Arc.1S genotypes, and their resistance to damage was apparently morphological (antixenotic) because they possessed structures that prevented contact between larvae and grains. The use of resistant genotypes in combination with other techniques may improve management of the weevil. Additionally, the resistant genotypes identified here can be used in breeding programs to develop common bean lines with resistance to A. obtectus.

Searching for common bean genotypes resistant to Acanthoscelides obtectus, a primary pest species of stored beans

“…Bragantia, Campinas, 83, e20230173, 2024 Baldin et al (2017) used the same method and reported that genotypes Arcelin 1, Arcelin 2, Arcelin 1S, Arcelin 5S, and Arcelin 3S showed antibiosis resistance against A. obtectus, corroborating with the results of the present study. Conversely, in the present study genotype Arcelin 3 was grouped with genotypes with the highest oviposition, the largest number of emerged insects, and the highest larval viability (Fig.…”

“…Arcelin is a protein toxic to several insects, including A. obtectus and Zabrotes subfasciatus (Mazzonetto and Vendramim 2002, Baldin and Pereira 2010, Baldin et al 2017, Tigist et al 2021. Currently, eight variants of arcelin have been identified in wild beans with different resistance levels to bruchids (Osborn et al 1986, Lioi and Bollini 1989, Cardona et al 1990, Santino et al 1991, Acosta-Gallegos et al 1998, Zaugg et al 2012).…”

Farmers traditional practices and management methods of stored common bean (Phaseolus vulgaris L.) insect pests in the central region of Benin Republic

“…This cosmopolitan insect pest has also been reported as the most important pest of stored beans in Asia (Thakur, 2012), America (Quentin et al, 1991;Silva andCosta, 2016 andBaldin et al, 2017), Africa (Rugumamu, 2014), Europa (Rugumamu, 2014) and Oceania (Daglish et al, 1993). In fact, larvae of A. obtectus enter the common bean seeds from the first instar stage and consume the reserves contained in the cotyledons which causes great losses (up to 30%) (Baldin et al, 2017). Infestation of stored common beans by the bean weevil gives a characteristic pungent odour, making them unfit for consumption and reducing their market value (Paul et al, 2009).…”

QTL mapping and identification of genes associated with the resistance to Acanthoscelides obtectus in cultivated common bean using a high-density genetic linkage map

“…The wild bean germplasms G12952 (ARC-4), G12949 (ARC-4) [ 32 ] and QUES (ARC-8) [ 40 ] are resistant to both A. obtectus and Z. subfasciatus . Baldin et al [ 41 ] screened 18 common bean genotypes of Andean American and Mesoamerican origin, and the common bean genotypes Arc.1, Arc.2, Arc.1S, Arc.3S, and Arc.5S were identified as resistant to A. obtectus . Some Bulgarian common bean genotypes and commonly grown Turkish bean genotypes were resistant against A. obtectus [ 42 , 43 ].…”