Effects of arbuscular mycorrhizal fungi and phosphorus fertilization on post vitro growth of micropropagated Zingiber officinale roscoe

Abstract

SUMMARYThe rhizomes of Zingiber officinale Roscoe (ginger) are widely used for their medicinal and flavoring properties, whereas the influence of root symbionts on their growth is poorly understood. In this study, the effects of phosphate fertilization and inoculation with a mixture of arbuscular mycorrhizal fungi (AMF) (isolates Glomus clarum RGS101A, Entrophospora colombiana SCT115A and Acaulospora koskei SPL102A) on survival, growth and development of micropropagated ginger were investigated. After transplanting to post vitro conditions, the ginger microplants were subjected to the following treatments: a) AMF mixture, b) P addition (25 mg kg -1 ), c) AMF + P, and d) non-mycorrhizal control without P addition. After eight months of growth, survival ranged from 86 to 100 % in the AMF and AMF+P treatments versus 71 % survival in control and P treatments. In the AMF, P and AMF+P treatments, the shoot, root and rhizome biomass production were significantly larger than in the control plants. In the nonmycorrhizal control plants the leaf number, leaf area, number of shoots/plants, and shoot length were significantly lower than in the AMF, P and AMF+P treatments. Root colonization ranged from 81 to 93 % and was not affected by P application. The data confirmed the response of several growth variables of micropropagated ginger to mycorrhizal colonization and P addition.

Growth, nutrient uptake and yield of ginger as impacted by potting media, foliar nutrition and microbial inoculants

“…This was followed by treatment T 6 (Trichoderma applied at 30 days interval throughout entire crop growth) which registered an yield of 523.33g plant -1 . Several workers have reported the significant effect of AMF in improving the yield of ginger (Santos 2010;Yunus et al 2014;Abbasi et al 2015;Li et al 2015;Hijri 2016). Beneficial effects of Trichoderma in improving crop growth has been Lopez-Bucio et al 2015).…”

Effect of Organic Manure and Arbuscular Mycorrhizal Fungi on Growth and Yield of Young Ginger (Zingiber officinale Rosc.)

“…Inoculation with an AMF mixture did not only strongly influence shoot, root and rhizome biomass production of ginger but also reduced or even replaced the chemical fertilizer input in the growth stages. This study showed that ginger is a plant with a high mycorrhizal dependency based on shoot biomass production (Santos et al, 2010).…”

The Effect of Arbuscular Mycorrhizal Fungi (AMF) Induction to The Growth and Atsiri Oils Content of Three Types Ginger (Zingiber officinale Rosc.)

“…Indeks luas daun merupakan perbandingan luas daun dan luas kanopi tanaman untuk menyatakan luas permukaan daun serta kempampuan daun dalam menyerap cahaya berdasarkan luas permukaannya. Menurut dos Santos et al (2010), nilai indeks luas daun yang tinggi dapat meningkatkan proses fotosintesis melaui penyerapan cahaya yang optimal akibat dari luas permukaan daunnya. Nilai ILD tertinggi di tunjukkan oleh perlakuan 15 g FMA/tanaman, sama halnya dengan tinggi tanaman pertumbuhan daun merupakan pertumbuhan vegetatif tanaman.…”