Isolation of Trypanosoma freitasi (Kinetoplastida: Trypanosomatidae) from Psychodopygus claustrei (Diptera: Psychodidae)

The molecular detection of different Leishmania species within sand flies from a cutaneous and visceral leishmaniasis sympatric area in Southeastern Brazil

“…We also knew that trypanosomatids other than Leishmania would be present in the wild sand flies (Wallace & Hertig 1968, Arias et al 1985, Naiff et al 1989) and recent studies (Rocha et al 2010) have shown that PCR-RFLP is unable to differentiate some Leishmania species from other trypanosomatids. In this study, the trypanosomatids that were present in the cortelezzii complex could be Leishmania spp other than L. infantum or L. braziliensis or other trypanosomatids that could be found in these sand flies.…”

“…Several studies using molecular methods to evaluate Leishmania infection rates in phlebotomines have been performed in Brazil (de Pita-Pereira et al 2005, Paiva et al 2006, Kato et al 2007, Saraiva et al 2009, Rocha et al 2010. In addition, studies in other trypanosomatids have been reported (Wallace & Hertig 1968, Arias et al 1985, Naiff et al 1989.…”

Phlebotomine Sandflies - Potential Vectors of Avian Trypanosomes

“…A putative trypanosome isolate from Psychodopygus claustrei was infective for an opossum (Didelphis marsupialis; Naiff et al 1989). Based on SSU rRNA sequence similarities of parasites present in sandflies and vertebrate hosts, sandflies were proposed as vectors of anuran and snake trypanosomes , Viola et al 2008, Kato et al 2010.…”

Molecular biological identification of monoxenous trypanosomatids and Leishmania from antropophilic sand flies (Diptera: Psychodidae) in Southeast Brazil

“…Several studies on natural infections of sand fly vectors with Leishmania and other trypanosomatids have been reported (Wallace and Hertig 1968;Arias et al 1985;Naiff et al 1989). Because direct methods to detect parasites in sand flies are limited, polymerase chain reaction (PCR)-based assays have been used for detecting Leishmaniainfected sand fly vectors in many regions where the disease is endemic (Paiva et al 2006;Kato et al 2007;Savani et al 2009).…”