Low cost production and purification of polyclonal antibodies to staphylococcal enterotoxin A

Abstract

An immunization scheme for production of antiserum to staphylococcal enterotoxin A (SEA) is proposed. The reference method of Robbins and Bergdoll was modified to reduce the number of doses and the amount of toxin used per animal. The best immunization scheme used injections in days 0, 8, 24, 59, 62 and 67. The amount of toxin at each injection was 5, 6, 20, 50, 100 and 200µg, respectively. The total amount of toxin was 381µg, which corresponded to a reduction of 107µg in the amount of toxin for each animal when compared to the reference method. The average antiserum titer using the Optimum Sensitivity Plate -OSP was 1:60 and using ELISA the titer was 1:100.000. The lack of cross-reactivity with other staphylococcal enterotoxins indicated high specificity of the antibody to SEA. The proposed immunization scheme was adequate to produce specific SEA antisera, with high titers and the aditional advantage of reducing the amount of purified SEA required for immunization.

Determinação Do Risco De Consumo De Leite Cru Relacionado À Intoxicação Estafilocócica

“…Polyclonal anti-sera for SEA were produced in the Laboratory of Food Science, Department of Food Technology and Medicine at the Centre of Agro-sciences of the State University of Londrina (OLIVEIRA & HIROOKA, 1999). The pure SEA toxin and standard lineages (FRI 722 and FRI A100) of SEA producing S. aureus were kindly provided from Food Research Institute, Wisconsin, USA.…”

"Listeria monocytogenes e Salmonella spp. em leite cru produzido em quatro regiões leiteiras no Brasil: ocorrência e fatores que interferem na sua detecção"

“…No Brasil não existem dados oficiais que revelem a real situação das toxinfecções alimentares no país . Muitas pesquisas revelam a presença de patógenos em vários alimentos estudados, porém a descrição de surtos e casos raramente é documentada (Pereira et al, 2001;Silva et al, 2001;Silva et al, 2000;Almeida Filho e Nader Filho, 2000;Mendes et al, 1999;Oliveira & Hirooka, 1999;Moura et al, 1993 Tanto em São Paulo como no Paraná não existem dados que relacionem exatamente o consumo de leite cru e derivados com a ocorrência de doenças. Entretanto, a qualidade microbiológica insatisfatória deste produto detectada em vários estudos (Bueno et al, 2002;Franco et al, 2000;Lourenço & Silva, 2000;Beloti et al, 1999;Poiatti et al, 1999) aureus produtor de enterotoxina Franco et al, 2000;Moura et al, 1993).…”