Determinação Do Risco De Consumo De Leite Cru Relacionado À Intoxicação Estafilocócica

Santana, Elsa Helena Walter de; Cunha, Maria de Lourdes Ribeiro de Souza da; Oliveira, Tereza Cristina Rocha Moreira de; Moraes, Luciane Bilia de; Aragón-Alegro, Lina Casale; Beloti, Vanerli

doi:10.5216/cab.v11i3.5751

Cited by 5 publications

(2 citation statements)

References 31 publications

(34 reference statements)

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“…Despite the high frequency of positive results, all counts were below 3.04 Log CFU / ml (table 2). Unlike the results found in this study, some authors have found contamination by SA in refrigerated raw milk with counts above 10 5 CFU/mL [5,23,26,30].…”

Section: Resultscontrasting

confidence: 99%

Evaluation of Microbiological Quality of Raw Milk Produced at Two Properties in the Far West of Santa Catarina, Brasil

Nádia¹,

Scapin²,

Oro³

et al. 2012

FPH

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Milk is a food that inherently favors microbial growth and due to its characteristics several precautions must be taken to prevent contamination in its production, processing, marketing and consumption, which are routinely subject to changes. The aim of this study was to evaluate microbiological contamination in milk produced at two farms in the Far West of Santa Catarina, before and after the application of Good Manufacturing Practices (GMP). Initially, samples of the milk, surfaces of equipment and utensils for milking, the teats of animals, disinfectants, and water were tested. Next, we conducted training of the farmers in microbiological analysis of milk samples. The analyses included counts of mesophilic aerobes (MA), Staphylococcus coagulase positive (SA), total coliform (TC), and thermotolerant (FC). The methods used for analyses were those described by the Regulation number 62 of August 26th, 2003 published by the Brazilian Ministry of Agriculture and Food Supply (MAPA) that follows methods recommended by the Compendium of Methods for the Microbiological Examination of Foods-APHA. The mean values for MA, SA, TC, and FC in milk obtained before and after the training were, respectively: 4.88 and 3.69 log colony-forming-unit (CFU)/ ml, 3.04 and 2.37 log CFU / ml, 61.19 Most probable number (MPN) and 17.89 MPN/ ml, and 40.26 and 8.71 MPN/ ml. Thus, according to these results, including training in GMP can improve the quality of milk, with immediate results for MA, TC, and CF. But, beyond the procedures employed, the control and prevention of mastitis could help to avoid contamination by SA.

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Section: Resultscontrasting

confidence: 99%

Evaluation of Microbiological Quality of Raw Milk Produced at Two Properties in the Far West of Santa Catarina, Brasil

Nádia¹,

Scapin²,

Oro³

et al. 2012

FPH

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“…A mastite é uma das enfermidades que mais causa prejuízos à produção e industrialização do leite, por reduzir a qualidade e a quantidade do produto [17,19]. Staphylococcus coagulase positiva (SCP), são os principais agentes etiológicos da mastite nos rebanhos [20], causando prejuízos aos produtores, à indústria e provocando riscos à saúde pública [22]. Sua virulência depende da produção de exotoxinas, proteínas de superfície e polissacarídeos extracelulares, sendo sua habilidade de aderência ao epitélio da glândula mamária associada à produção de biofilmes [11], que confere uma vantagem fisiológica na sua possível atuação como agente etiológico [13].…”

Section: Introductionunclassified

Biofilm Production and In Vitro Susceptibility to Disinfectants of Staphylococcus Coagulase Positive Isolated from Bovine Milk

Peixoto

Medeiros

Mota

et al. 2019

Acta Scientiae. Vet.

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Background: Mastitis is one of the diseases that cause damage to dairy cattle, and coagulase positive Staphylococcus (CPS), are its main etiological agents. The production of biofilms gives these microorganisms a physiological advantage, being an important virulence factor, as it confers their resistance to the action of disinfectants used in hygiene procedures. Therefore, the objective of this study was to evaluate the capacity of CPS, isolated from milk samples from cases of bovine subclinical mastitis in the Alagoas dairy basin, biofilm production and its sensitivity to disinfectant agents used before and after dipping.Materials, Methods & Results: In the Alagoas Milk Basin Region, 10 properties were selected from the municipalities of Batalha, Cacimbinhas, Major Isidoro and Minador do Negrão, which used a mechanical milking system and supplied milk to a dairy under federal inspection. The California Mastitis Test (CMT) was performed on 1155 cows. The animals diagnosed with subclinical mastitis were classified as: mild (+); moderate (++) and severe (+++). CMT positive samples were collected from 2+, totaling 891 samples. After cleaning the papillary ostium, 2 to 5 mL of milk was collected from each fourth breast before milking began. Subsequently, 0.5 µL of each sample was streak plated by exhaustion on 5% Sheep Blood Agar and incubated at 37°C, with readings at 24 and 48 h. The macroscopic and microscopic characteristics of the colonies, catalase and coagulase test were evaluated. Biofilm formation was determined by the microplate adhesion test. The efficacy of the sanitizers was evaluated with the active ingredients: lactic acid (2%), allantoin (0.05%), iodine (0.5%), chlorhexidine (2.0%), chlorine (2.5%), at times 15, 30 and 60 seconds. It was observed that 91.2% of the samples were able to form biofilm; Of these, 22.2%, 29.6% and 48.2% were strong, moderate and weak producers, respectively. The sensitivity profile of chlorhexidine isolates was 79.73%, 80.41% and 83.78%; iodine was 59.46%, 65.54% and 66.22% sensitivity over the evaluated time. For allantoin, the isolates presented sensitivity of 22.30%, 27.03% and 37.74%, for lactic acid, 18.92%, 20.95% and 22.30% of the samples were sensitive, and to chlorine, 12.6%, 15.58% and 17.57% of the isolates were sensitive. Moreover, it was observed that the longer the exposure to the disinfectant, the greater the effectiveness of the same.Discussion: The results are in line with literature data. Staphylococcus spp. from milk samples of animals with subclinical mastitis are able to form biofilm, thus being an important virulence factor. The heterogeneity regarding the biofilm production potential of the isolates is due to the different species of Staphylococcus spp. that, through distinct mechanisms, triggers a greater or lesser formation of this virulent factor, as well as having their adhesion tested, end up becoming more strongly attached to the plate. Among the disinfectants tested, it was confirmed that chlorhexidine and iodine are more effective than SCP, which tend to have high levels of sensitivity to these disinfectants. However, although chlorhexidine was more sensitive to SCP, its use in the field should be done with caution, as its effectiveness may be reduced in the presence of chlorinated water or excess organic matter. It is concluded that SCP isolates from bovine milk from subclinical mastitis mammary quarters were able to produce biofilms. These isolates were also in vitro sensitive to chlorhexidine and iodine, but periodic evaluation of the efficacy of disinfectants used before and after milking is necessary.

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Isolation and Identification of Biofilm-Forming Staphylococcus Aureus in Commercial Cow Milk Products

Yambise¹,

Ariestanti²,

Budiarso³

2020

Scita.

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Food poisoning caused by the contamination from Staphylococcus aureus are frequently found in foodespecially in dairy products. Pasteurization process in milk production was not enough to kill S. aureus becauseit formed biofilm that could survive in high temperature. This research aimed to study the presence of biofilmforming S. aureus in samples from packed commercial milk products in Yogyakarta City, Indonesia. Twentyisolates from dairy products were grown in Brain Heart Infusion (BHI) broth then inoculated into Braid-ParkerAgar (BPA) medium to get the candidate of S. aureus isolates. These isolate candidates were selected usingMannitol Salt Agar (MSA) and Congo Red Agar (CRA) medium. Another selection was done by carbohydratefermentation analysis and confirmed using API STAPH. Confirmation analysis showed that eight isolateswere identified as S. aureus. Another two isolates were identified as S. xylosus and S. haemolyticus. Therefore,it indicated the presence of Staphylococcus aureus as contaminant in dairy products.

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Determinação Do Risco De Consumo De Leite Cru Relacionado À Intoxicação Estafilocócica

Cited by 5 publications

References 31 publications

Evaluation of Microbiological Quality of Raw Milk Produced at Two Properties in the Far West of Santa Catarina, Brasil

Evaluation of Microbiological Quality of Raw Milk Produced at Two Properties in the Far West of Santa Catarina, Brasil

Biofilm Production and In Vitro Susceptibility to Disinfectants of Staphylococcus Coagulase Positive Isolated from Bovine Milk

Isolation and Identification of Biofilm-Forming Staphylococcus Aureus in Commercial Cow Milk Products

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