Production and Purification of Polyclonal Antibody Against Human Kappa Light Chain

Abdolalizadeh, Jalal; Majidi, Jafar; Farajnia, Safar

doi:10.3923/jbs.2008.683.686

Cited by 5 publications

(3 citation statements)

References 26 publications

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“…ELISA test used for labeling antibody (Folks and Wardaman, 2001;Ramadan et al, 2011) and also, the produced antibody has many application in research, clinic and education. This polyclonal antibody can be used for diagnosis and monitoring of free light chain producing diseases (Abdolalizadeh et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Production and purification of horseradish peroxidase in Pakistan

Khurshid¹,

Qureshi²,

Ibrahim³

et al. 2012

Int. J. Phys. Sci.

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Horseradish peroxidase is heme containing enzyme belonging to oxidoreductases and is involved in degradation of certain recalcitrant organic compounds like phenol and substituted phenol via free radical polymerization. Peroxidase was extracted and purified from horseradish by (NH 4) 2 SO 4 precipitation, dialysis and gel filtration chromatography. Spectrophotometer was used for the assay of horseradish peroxidase in this present project. Horseradish peroxidase increased activity from 6.3027 to 9.9452 U/ml by purification. It gained 45.77 folds purification at this final step. It is helpful in determination of glucose in blood and compounds in it kill bacterial strains.

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Section: Introductionmentioning

confidence: 99%

Production and purification of horseradish peroxidase in Pakistan

Khurshid¹,

Qureshi²,

Ibrahim³

et al. 2012

Int. J. Phys. Sci.

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“…67,68 Among them the anion exchange method has the most usage. 66 Three steps of conventional method for IEC consisting pre-treatment, purification and polishing was shown in Figure 4.…”

Section: Ion Exchange Chromatography (Iec)mentioning

confidence: 99%

Overview of Albumin and Its Purification Methods

et al. 2016

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“…This polyclonalantibody can be used for diagnosis and monitoringof free light chain producing disease. [6][7] The obvious values of peroxidases, their present commercial uses are limited, primarily by the low stability ofperoxidases in the presence of their natural substrate, hydrogenperoxide. Presence of high concentrations of hydrogen peroxideled to inactivation of hemoproteins, including peroxidases.…”

Section: Introductionmentioning

confidence: 99%

Extraction and Purification of Localyhorseradish Peroxidase

Yaseen¹

2017

WJPPS

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Horseradish peroxidase is a comprising enzyme which canbe appropriate to oxidoreductases and is intricate indegradation of definiteintractable organic complexes like phenol and substituted phenol via freeradical polymerization. Peroxidase was extracted and purified from horseradish usingammonium sulphatefor dialysis, gel filtration and precipitationchromatography. Spectrophotometer was used for essay ofhorseradish peroxidase in this present research. It was concluded that horseradish peroxidase increased activity by purification. In addition to itsbeneficial incharacterization of glucose in blood and complexes; moreover it can destroymicrobial strains.

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Production and Purification of Polyclonal Antibody Against Human Kappa Light Chain

Cited by 5 publications

References 26 publications

Production and purification of horseradish peroxidase in Pakistan

Production and purification of horseradish peroxidase in Pakistan

Overview of Albumin and Its Purification Methods

Extraction and Purification of Localyhorseradish Peroxidase

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