Overview of Albumin and Its Purification Methods

Raoufinia, Ramin; Mota, Ali; Keyhanvar, Neda; Safari, Fatemeh; Shamekhi, Sara; Abdolalizadeh, Jalal

doi:10.15171/apb.2016.063

Cited by 116 publications

(81 citation statements)

References 116 publications

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“…Proteins were sorted into classes according to fit by the following criteria. Albumins are globular proteins that bind solutes such as fatty acids and assist in maintaining oncotic pressure . Apolipoproteins bind to lipids (forming lipoproteins) to allow transport .…”

Section: Resultsmentioning

confidence: 99%

“…Albumins are globular proteins that bind solutes such as fatty acids and assist in maintaining oncotic pressure. 19 Apolipoproteins bind to lipids (forming lipoproteins) to allow transport. 20 Complement components trigger the innate immune system, 21 and enzymes catalyze a wide range of reactions.…”

Section: Resultsmentioning

confidence: 99%

“…Some protein classes will have a greater effect than others on interactions with materials. It is unlikely that albumins will have any long‐term effect, as they do not specifically bind to surfaces and will not be dominant at the surface of a chronic implant . Apolipoproteins do not bind strongly with platinum, making their relatively high content in animal models a minor consideration.…”

Section: Discussionmentioning

confidence: 99%

“…It is unlikely that albumins will have any long-term effect, as they do not specifically bind to surfaces and will not be dominant at the surface of a chronic implant. 19 Apolipoproteins do not bind strongly with platinum, 20 making their relatively high content in animal models a minor consideration. Glycoproteins have oligosaccharide side chains that affect their biological functions, including binding properties.…”

Section: Discussionmentioning

confidence: 99%

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Comparing perilymph proteomes across species

et al. 2017

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Objectives/Hypothesis: Biological components of perilymph affect the electrical performance of cochlear implants. Understanding the perilymph composition of common animal models will improve the understanding of this impact and improve the interpretation of results from animal studies and how it relates to humans.Study Design: Analysis and comparison of the proteomes of human, guinea pig, and cat perilymph. Methods: Multiple perilymph samples from both guinea pigs and cats were analysed via liquid chromatography with tandem mass spectrometry. Proteins were identified using the Mascot database. Human data were obtained from a published dataset. Proteins identified were refined to form a proteome for each species.Results: Over 200 different proteins were found per species. There were 81, 39, and 64 proteins in the final human, guinea pig, and cat proteomes, respectively. Twenty-one proteins were common to all three species. Fifty-two percent of the cat proteome was found in the human proteome, and 31% of the guinea pig was common to human. The cat proteome had similar complexity to the human proteome in three protein classes, whereas the guinea pig had a similar complexity in two. The presence of albumin was significantly higher in human perilymph than in the other two species. Immunoglobulins were more abundant in the human than in the cat proteome.Conclusions: Perilymph proteomes were compared across three species. The degree of crossover of proteins of both guinea pig and cat with human indicate that these animals suitable models for the human cochlea, albeit the cat perilymph is a closer match.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Comparing perilymph proteomes across species

et al. 2017

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“…Therefore, the assessment of biocompatibility in terms of protein fouling seems essential for the future use of microgrippers for biomedical applications. Among proteins, serum albumin is the most abundant in human blood, accounting for more than 50% of total proteins [32,33]. The presence of albumin in the extravascular compartment is also significant.…”

Section: Protein Fouling At the Microgripper Surfacesmentioning

confidence: 99%

Innovative Silicon Microgrippers for Biomedical Applications: Design, Mechanical Simulation and Evaluation of Protein Fouling

et al. 2018

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Abstract:The demand of miniaturized, accurate and robust micro-tools for minimally invasive surgery or in general for micro-manipulation, has grown tremendously in recent years. To meet this need, a new-concept comb-driven microgripper was designed and fabricated. Two microgripper prototypes differing for both the number of links and the number of conjugate surface flexure hinges are presented. Their design takes advantage of an innovative concept based on the pseudo-rigid body model, while the study of microgripper mechanical potentialities in different configurations is supported by finite elements' simulations. These microgrippers, realized by the deep reactive-ion etching technology, are intended as micro-tools for tissue or cell manipulation and for minimally invasive surgery; therefore, their biocompatibility in terms of protein fouling was assessed. Serum albumin dissolved in phosphate buffer was selected to mimic the physiological environment and its adsorption on microgrippers was measured. The presented microgrippers demonstrated having great potential as biomedical tools, showing a modest propensity to adsorb proteins, independently from the protein concentration and time of incubation.

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Liver Function in Health and Disease

Mells

Alexander²

2018

Sherlock's Diseases of the Liver and Biliary System

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Overview of Albumin and Its Purification Methods

Cited by 116 publications

References 116 publications

Comparing perilymph proteomes across species

Comparing perilymph proteomes across species

Innovative Silicon Microgrippers for Biomedical Applications: Design, Mechanical Simulation and Evaluation of Protein Fouling

Liver Function in Health and Disease

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