Atividade de nanoformulações de Melaleuca alternifolia e terpinen-4-ol em isolados de Rhodococcus equi

Sagave, Lauren; Gressler, Luciane Tourem; Flôres, Fabíola Niederauer; Silva, C. B.; Vargas, Águeda Castagna de; Lovato, Maristela; Sangioni, Luís Antonio; Pötter, Luciana; Botton, Sônia de Ávila

doi:10.1590/1678-7454

Cited by 14 publications

(7 citation statements)

References 17 publications

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“…9 One of the possible benefits of using nanostructures is the decrease of the concentration in the formulation, presenting equal or greater effect than the usual formulation, as previously demonstrated with chlorhexidine nanocapsules 19,54 and vegetable oil. 15 Consequently, our group decided to compare a lowconcentrated nanostructured formulation to a consolidated formulation, resulting in similar effects both for NM-Cl and for CS-Cl, demonstrating that the use of nanoformulations could decrease chlorhexidine concentrations.…”

Section: In Vivo Assaymentioning

confidence: 99%

“…14 Increasing reports of resistance to chlorhexidine formulation are the main reason to the current demand of the investigation of antiseptic alternatives. Nanostructured formulations are believed to be an alternative for hospital antiseptics, 7,14,15 and among them, the nanoemulsions are heterogeneous dispersions of immiscible liquids in droplet form, stabilized by interfacial surfactant film usually in combination with surfactant molecules. [17][18][19][20][21][22][23][24][25][26][27][28][29] Nanoparticles have been widely studied as potential carriers for antimicrobials, and some advantages compared to conventional antibacterials are: the contour to the resistance mechanisms, mainly due to the size, as it results in a greater interaction due to the surface area; impediment of biofilm formation; targeting to the site of infection and consequently, minimizing side effects; controlled and sustained release and association of antimicrobials in a single nanoparticulate system.…”

Section: Introductionmentioning

confidence: 99%

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<p>Chlorhexidine Nanoemulsion: A New Antiseptic Formulation</p>

Risso

Stopiglia

Oliveira

et al. 2020

IJN

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Introduction: Nanoparticle solutions have been studied to improve antimicrobial effect. The aim of this study was to develop, characterize, and evaluate the in vitro and in vivo antiseptic efficacy of 0.25% aqueous-based chlorhexidine nanoemulsion (NM-Cl 0.25% w/v). Methods: The NM-Cl 0.25% w/v (2.5mg/mL) and free chlorhexidine nanoemulsion (FCN; same composition of NM-Cl without the molecule of chlorhexidine) were synthetized by the spontaneous emulsification method. Characterization analyses of physical and chemical properties were performed. The NM-Cl 0.25% w/v was compared with chlorhexidine 0.5% alcohol base (CS-Cl 0.5%) in vitro studies (microdilution study and kill curve study), and in vivo study (antisepsis of rats dorsum). Kruskal-Wallis test was used between groups and inside the same group, at different sample times and the Mann-Whitney test was performed when difference was detected. Results: The NM-Cl 0.25% w/v presented adequate physicochemical characteristics for a nanoemulsion, revealing a more basic pH than FCN and difference between zeta potential of NM-Cl 0.25% w/v and FCN. The NM-Cl 0.25% w/v and CS-Cl 0.5% solutions were more effective on Gram-positive than on Gram-negative bacteria (p≤0.05). NM-Cl 0.25% w/v presented upper antiseptic effect in the microdilution study and residual antiseptic effect was maintained for a longer time when compared to CS-Cl 0.5% (kill curve study). The four-fold (minimal inhibitory concentration) of NM-Cl 0.25% were the formulations with most durable effect within those tested, presenting residual effect until T6 for both bacteria. In the in vivo study, both formulations (NM-Cl 0.25% w/v and CS-Cl 0.5%) had a reduction of the microorganisms in the skin of the rats (p<0.0001) not revealing any difference between the formulations at different times, showing the antiseptic effect of NM-Cl (p≤0.05). Conclusion: Both in vitro and in vivo experiments demonstrated that NM-Cl showed promising future as an antiseptic for cutaneous microbiota.

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Section: In Vivo Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

<p>Chlorhexidine Nanoemulsion: A New Antiseptic Formulation</p>

Risso

Stopiglia

Oliveira

et al. 2020

IJN

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“…Sagave et al . (2015) studied the antimicrobial activity of TTO against a Rhodococcus equi isolate, and reported that the activity of the oil was enhanced when used as nanoformulation. Nanotechnology is an important feature of the pharmaceutical industry, especially in the development of more efficient formulations that can remain for long periods at the action site (Putheti et al .…”

Section: Discussionmentioning

confidence: 99%

In vitro and ex vivo activity of Melaleuca alternifolia against protoscoleces of Echinococcus ortleppi

et al. 2016

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Cystic echinococcosis is a zoonotic disease of difficult diagnosis and treatment. The use of protoscolicidal agents in procedures is of utmost importance for treatment success. This study was aimed at analysing the in vitro and ex vivo activity of Melaleuca alternifolia oil (tea tree oil - TTO), its nanoemulsion formulation (NE-TTO) and its major component (terpinen-4-ol) against Echinococcus ortleppi protoscoleces obtained from cattle. Concentrations of 2·5, 5 and 10 mg mL-1 of TTO, 10 mg mL-1 of NE-TTO and 1, 1·5 and 2 mg mL-1 of terpinen-4-ol were evaluated in vitro against protoscoleces at 5, 10, 15 and 30 min. TTO was also injected directly into hydatid cysts (ex vivo analysis, n = 20) and the viability of protoscoleces was evaluated at 5, 15 and 30 min. The results indicated protoscolicidal effect at all tested formulations and concentrations. Terpinen-4-ol (2 mg mL-1) activity was superior when compared with the highest concentration of TTO. NE-TTO reached a gradual protoscolicidal effect. TTO at 20 mg mL-1 showed 90% protoscolicidal action in hydatid cysts at 5 min. The results showed that TTO affects the viability of E. ortleppi protoscoleces, suggesting a new protoscolicidal option to the treatment of cystic equinococcosis.

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“…The chemical components of essential oils (EOs) from medicinal plants have antibacterial (Sagave et al . ), antifungal (Cleff et al . ,b) and antiparasitic (Santos et al .…”

Section: Introductionmentioning

confidence: 99%

“…The chemical components of essential oils (EOs) from medicinal plants have antibacterial (Sagave et al 2015), antifungal (Cleff et al 2010a,b) and antiparasitic (Santos et al 2012) properties, and they act as insect repellents (Lee et al 2007). Moreover, previous studies have reported Pythium spp.…”

Section: Introductionmentioning

confidence: 99%