In vitro and ex vivo activity of Melaleuca alternifolia against protoscoleces of Echinococcus ortleppi

Monteiro, Danieli Urach; Azevedo, María Isabel de; Weiblen, Carla; Botton, Sônia de Ávila; Funk, Nadine Lysyk; Silva, Cristiane de Bona da; Zanette, Régis A.; Schwanz, Thiago Guilherme; Rue, Mario Luiz de la

doi:10.1017/s0031182016001621

Cited by 17 publications

(7 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Fungal chitosan seems to have stronger scolicidal effects due to beneficial characteristics, such as easy access, low-cost culture, nutrient and processing; however, further in vivo and experimental studies are still needed to attain precise results [40]. In vitro and ex vivo activity of Melaleuca alternifolia oil (tea tree oil, TTO) at 20 mg/ml indicated 90% protoscolicidal action in E. ortleppi hydatid cysts at five minutes [41]. Mahmoudvand et al [42] showed that Zataria multiflora Boiss essential oil caused a significant scolicidal effect (100%) at the concentrations of 12.5 and 6.25 μl/ml after 5 and 20 minutes of exposure, respectively [42].…”

Section: Discussionmentioning

confidence: 99%

Nanostructured lipid carriers of ivermectin as a novel drug delivery system in hydatidosis

et al. 2019

View full text Add to dashboard Cite

BackgroundThe larval stage of the tapeworm Echinococcus granulosus is the causative agent of hydatid disease in humans. This zoonotic parasitic infection remains a major health problem in certain areas of the world where is still endemic. In view of the ineffectiveness of some drug treatments, the surgical removal of cysts remains the preferred treatment option together with the administration of albendazole and mebendazole. However, severe side effects of these drugs have been reported which demands developing new scolicidal agents that confer suitable efficacy and fewer side effects during surgery.MethodsTo that purpose, in the present work we assessed the effectiveness of ivermectin (IVM), a macrocyclic lactone endectocide that has shown to be an effective nematocidal drug against other important parasitic infections. To overcome the limitations observed in some drug formulations and resistance, we used nano lipid carriers (NLCs) as a targeted and sustained drug delivery system for IVM. We evaluated the in vitro cestocidal and apoptotic effects of NLCs-loaded IVM versus IVM by quantifying the expression of caspase-3 mRNA.ResultsWe found that after 60 and 120 min of administration, 800 μg/ml and 400 μg/ml NLCs-loaded IVM induced 100% mortality, respectively. On the other hand, the 800 μg/ml of IVM induced 100% mortality rate 150 min after administration. Additionally, we found that NLCs-loaded IVM induced higher mRNA caspase-3 expression suggesting a more potent apoptotic effect on the parasite.ConclusionsThese data suggest that NLCs-loaded IVM may be a promising alternative to current treatments although in vivo studies are needed.

show abstract

Section: Discussionmentioning

confidence: 99%

Nanostructured lipid carriers of ivermectin as a novel drug delivery system in hydatidosis

et al. 2019

View full text Add to dashboard Cite

show abstract

“…In the next step, an aliquot from the liquid containing the protoscoleces was collected at 5, 10, 20, and 30 min after exposure. Subsequently and similar to the in vitro proto scolicidal assay, the viability of protoscoleces was determined by the eosin exclusion test [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

Efficacy and Safety Curcuma zadoaria L. to Inactivate the Hydatid Cyst Protoscoleces

Mahmoudvand

Pakravanan

Kheirandish

et al. 2020

CCP

View full text Add to dashboard Cite

Background: The present work aimed to evaluate the chemical composition of Curcuma zadoaria essential oil and to investigate its efficacy and safety against hydatid cyst protoscoleces. Methods: Collected protoscoleces from liver fertile hydatid cysts of infected sheep were exposed to different concentrations of the essential oil (75, 150, 300 μl/mL) for 5-30 min in vitro and ex vivo. Then, by using the eosin exclusion assay, the viability of protoscoleces was studied. In the next step, 24 male NMRI mice were examined to assess the toxicity of C. zadoaria essential oil by measuring the biochemical and hematological parameters. Results: Based on the obtained results, the LD50 value of intraperitoneal injection of the C. zadoaria essential oil was 1.76 mL/kg of body weight and the maximum non-fatal dose was 0.96 mL/kg of body weight. C. zadoaria essential oil had a strong proto scolicidal activity in vitro so that at the 300 and 150 μl/ml entirely eliminates the parasite after 5 and 10 minutes; whereas, weak proto scolicidal activity was observed at lower doses. Ex vivo assay, no similar effect with in vitro was observed, therefore, more time is required to show a potent proto scolicidal activity. C. zadoaria essential oil at the concentrations of 300 and 150 μl/mL after an exposure time of 7 and 12 min, killed 100% of protoscoleces within the hydatid cyst, respectively. After intraperitoneal injection of the C. zadoaria essential oil for 2 weeks, no significant difference (p > 0.05) was observed in the clinical chemistry and hematologic parameters at the doses of 0.15, 0.3, 0.6 mL/kg. Conclusion: The obtained results in vitro and ex vivo exhibited that C. zadoaria essential oil had a favorable proto scolicidal activity on hydatid cyst protoscoleces. However, more supplementary works are required to verify these findings by assessing clinical subjects.

show abstract

“…In the next step, an aliquot from the liquid containing the protoscoleces was collected at 7, 10, 12,15, 20, 25 and 30 min after the exposure. Subsequently and similar to the in vitro protoscolicidal assay, the viability of protoscoleces was determined by eosin exclusion test [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

In vitro and ex vivo scolicidal effects of Olea europaea L. to inactivate the protoscolecs during hydatid cyst surgery

Niazi

Saki

Sepahvand

et al. 2019

Annals of Medicine and Surgery

View full text Add to dashboard Cite

Background Nowadays, surgery is considered as one of the most important treatments for hydatidosis. Due to laceration, the cyst and spread of the content within it (protoscoleces) during the surgery that can put the patient at the risk of re-infection, anaphylaxis shock and even death, surgeons use some chemical drugs as protoscolicidal agents. The study is aimed to evaluate the scolicidal effects of olive ( Olea europaea L.) leaf extract on hydatid cyst protoscoleces in vitro and ex vivo . Methods After the collection of protoscoleces from sheep livers infected with fertile hydatid cysts, they were treated with various concentrations of olive leave extract (75–300 mg/mL) for 5–30 min in vitro and ex vivo . Finally, the mortality of protoscoleces was assessed by the eosin exclusion test (0.1% eosin staining). Results The mean of the mortality of protoscoleces was 100% after 10 min of incubation with the concentration of 300 mg/ml of O. europaea leaves extract. On the other hand, the mean of the mortality of protoscoleces after 20 min of incubation with the concentration of 150 mg/ml of O. europaea leaves extract was 100%. After injection of O. europaea leaves extract directly into the hydatid cyst ( ex vivo ), the mean of the mortality of protoscoleces was 100% after 12 and 25 min of incubation with the concentration of 300 and 150 mg/ml of O. europaea leaves extract, respectively; indicating that the extract requiring a further time to display a potent protoscolicidal effects. Conclusion Based on the findings of the study, it can be concluded that the extract of olive leaf had a significant scolicidal activity on hydatid cyst protoscoleces. However, further research, especially in human and animal subjects, are required to reach this conclusion.

show abstract

In vitro and ex vivo activity of Melaleuca alternifolia against protoscoleces of Echinococcus ortleppi

Cited by 17 publications

References 35 publications

Nanostructured lipid carriers of ivermectin as a novel drug delivery system in hydatidosis

Nanostructured lipid carriers of ivermectin as a novel drug delivery system in hydatidosis

Efficacy and Safety Curcuma zadoaria L. to Inactivate the Hydatid Cyst Protoscoleces

In vitro and ex vivo scolicidal effects of Olea europaea L. to inactivate the protoscolecs during hydatid cyst surgery

Contact Info

Product

Resources

About