Morfometria corpórea, características do sêmen, proteínas seminais e testosterona em cervos Cervus unicolor, em cativeiro

Abstract

RESUMOObjetivou-se estudar a morfometria corpórea, as características do sêmen, o perfil proteico do plasma seminal em SDS-PAGE e a concentração sérica de testosterona em cervos-sambar (Cervus unicolor), criados em cativeiro, na estação reprodutiva da primavera. Quatro machos com idades entre 12 e 36 meses foram avaliados em quatro momentos, com intervalos de sete dias, com peso corpóreo (60,5 a 89,0kg), índice de massa corporal (93,07kg/m 2 a 126,56kg/m 2 ), volume do ejaculado (0,50±0,35mL a 0,75±0,28 mL), motilidade espermática (87,75±4,78% a 90,00±7,07%), defeitos totais (17,25±5,81% a 47,72±17,55%), testosterona sérica (6,43±4,33ng/dL a 166,00±64,48ng/dL) e proteínas do plasma seminal com bandas entre 7,6 e 142kDa. As características dos ejaculados não diferiram (P>0,05) entre as três primeiras colheitas. Houve diferença (P<0,05) para os defeitos espermáticos com elevação na quarta colheita. No plasma seminal de cada cervo, foram identificadas de 16 a 27 bandas de proteínas entre 7,6 e 142kDa. Conclui-se que a qualidade espermática foi satisfatória na primavera. O estresse das contenções sucessivas causou queda da qualidade espermática. A idade influi na concentração sérica de testosterona, a qual foi maior nos cervos aos 36 meses.Palavras-chave: Cervidae, cativeiro, sêmen, SDS-PAGE, andrógeno (60.5 to 89.0kg), body mass index (93.07 to 126.56kg/m 2 ), ejaculate volume (0.50±0.35mL to 0.75±0.28mL), sperm motility (87.75±4.78% to 90.00±7.07% ), total defects (17.25±5.81% to 47.72±17.55%), serum testosterone (6.43±4.33 ng/dL to 166.00±64.48ng/dL) ABSTRACT The aim of this work was to study the body morphometry, semen characteristics, seminal plasma protein profile in SDS-PAGE and serum testosterone concentration in Sambar Deer (Cervus unicolor), in captivity in the breeding season (spring). Four males aged between 12 and 36 months were assessed in four moments with intervals of seven days with body weight

Characterization of seminal parameters, sperm morphometry, micromorphology, and ultrastructure in gray brocket deer (Mazama gouazoubira, Fischer, 1814)

“…The measurements obtained in the current study reflect these values. Similar results were obtained for other cervids, including Dama dama , Odocoileus virginianus , Elaphodus cephalophus , C. unicolor , and M. nana (Chacur et al, 2014; de Abreu et al, 2009; Fernández, Sestelo, Rivolta, & Córdoba, 2013; Kirschner & Rodenkirch, 2017; Panyaboriban et al, 2016).…”

“…For M. nana and M. americana , mean values for motility and vigor were around 70% and 3, respectively, in fresh semen samples (de Abreu et al, 2009; Rola et al, 2013). The mean mass movement for M. americana and Cervus unicolor was around 3 and 4, respectively (Chacur et al, 2014; Rola et al, 2013). Thus, sperm of gray brocket deer sperm has good antioxidant ability (Darr et al, 2016), mitochondrial integrity (Paoli et al, 2011), and effective osmolarity control capacity (Cooper & Yeung, 2003).…”

“…The current study used Blom's classification for morphological analysis (Blom, 1972), because it is widely used for several species (Azerêdo, Esper, & Resende, 2001; Chacur et al, 2014; Enciso et al, 2011; Gizejewski, 2004; Tsuneda et al, 2015). This classification also provides the proportion of major and minor defects, with the latter being the least deleterious to fertility (Blom, 1972).…”

Bromatological and Sensorial Evaluation of Pereskia Aculeata Use in Pasta Dough

“…Such data can be seen in Figure 7. Statistical analysis adopted in this work were established such as in Pereira et al (2013) and Chacur et al (2012Chacur et al ( , 2013Chacur et al ( , 2014.…”

Characterization and Celular Proliferation of Ovarian Structures of Ewes by the Technique of Agnor: Ovarian Morfometry and Correlations

“…Data analysis of the ovarian morphology was obtained from the results of the averages that were submitted, for groups with normal distribution, to analysis of variance by F test, according Cremasco et al (2009), for paired observations as completely randomized design, statistical analysis similar to that performed in animal science area in Gabriel Filho et al (2011), Chacur et al (2012Chacur et al ( , 2013Chacur et al ( , 2014 and Castilho et al (2013Castilho et al ( , 2015.…”