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(2015) Semen quality, testicular B-mode and Doppler ultrasound, and serum testosterone concentrations in dogs with established infertility. Theriogenology, 84 (5). pp. 805-810. ISSN 1879-3231 Access from the University of Nottingham repository: http://eprints.nottingham.ac.uk/38920/1/Dogs%20with%20Established%20Infertility %20Theriogenology.pdf Copyright and reuse:The Nottingham ePrints service makes this work by researchers of the University of Nottingham available open access under the following conditions. This article is made available under the Creative Commons Attribution Non-commercial No Derivatives licence and may be reused according to the conditions of the licence. For more details see: http://creativecommons.org/licenses/by-nc-nd/2.5/ A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription. concentrations (1.40 ± 0.62 ng / mL) than fertile dogs (1.81 ± 0.87 ng / mL) (P < 0.05). 37There were subjective differences in testicular echogenicity in some of the infertile 38 dogs, and important differences in testicular artery blood flow with lower peak systolic 39 and end diastolic velocities measured in the distal supra-testicular artery, marginal 40 testicular artery and intra-testicular artery of infertile dogs (P < 0.05). Notably, 41resistance index and pulsatility index did not differ between infertile and fertile dogs. 42These findings demonstrate important differences between infertile and fertile dogs 43 which may be detected within an expanded breeding soundness examination.

RESUMO.-[Avaliação Triplex Doppler dos testículos de cães de tamanhos diferentes.]Este trabalho teve como objetivo verificar se existem diferenças nos parâmetros dopplervelocimétricos entre cães de diferentes portes. Para tanto, foram utilizados 20 cães, sendo 10 cães de pequeno porte e 10 cães de grande porte. Foram avaliados pelo Triplex Doppler para localização da artéria testicular nos segmentos de cordão espermático, marginal ao testículo e intratesticular. Após isso, o Doppler espectral foi acionado para cálculo dos parâmetros de velocidade de pico sistólico (VPS), velocidade diastólica final (VDF) e índices de resistência (IR) e pulsatilidade (IP). O volume testicular médio do testículo esquerdo foi significativamente maior que o direito em ambos os grupos. Ao Doppler, foram observadas diferenças dos valores entre os portes, sendo as velocidades no cordão espermático superiores nos animais de grande porte (P < 0,05) e dentro dos grupos também foram observadas diferenças entre as regiões da artéria testicular. Diante do exposto, conclui-se que existem diferenças nos parâmetros dopplervelocimétricos quando comparados animais de diferentes portes, além disso, os parâmetros dopplervelocimétricos são diferentes dependendo da região em que são mensurados. INTRODUCTIONThe ultrasonographic evaluation of the testes allows the study of anatomy, parenchyma and adjacent structures, which is important to detect anatomical abnormalities, as well as guiding interventional procedures for diagnosis purposes (Nyland & Matton 2004). Two-dimensional ultrasound of the testes enables the assessment of echogenicity and echotexture, besides the measure of testicular volume, which may represent normal development of testes, serving as a tool in detecting testicular diseases (Dogra et al. 2003). This study aimed to assess whether there are differences in Doppler velocimetry parameters between different sizes. Twenty dogs were equally divided into small and large groups used in this study. The dogs were evaluated using Triplex ultrasound. Testicular artery was located by Colour Doppler in the spermatic cord, marginal to the testes and intratesticular segments and then, spectral Doppler were used to calculate: peak systolic velocity (PSV), end diastolic velocity (EDV), resistance index (RI) and pulsatility index (PI). The mean testicular volume in the left side was significantly higher than the right side, in both groups. Doppler examination showed higher velocities (EDV) at spermatic cord in large dogs; marginal to the testes was observed higher velocities in small dogs; intratesticular region no differences were observed (P < 0.05) and within the groups differences between segments of the artery were also observed for each parameter. The results showed that there are differences in Doppler velocimetry parameters between different sizes.

(2015) Digital image analysis of testicular and prostatic ultrasonographic echogencity and heterogeneity in dogs and the relation to semen quality. Animal Reproduction Science, 160 . pp. 112-119. ISSN 1873-2232 Access from the University of Nottingham repository: http://eprints.nottingham.ac.uk/38918/1/Image%20analysis%20testes%20and%20prostate %20Animal%20Reproduction%20Science.pdf Copyright and reuse:The Nottingham ePrints service makes this work by researchers of the University of Nottingham available open access under the following conditions. This article is made available under the Creative Commons Attribution Non-commercial No Derivatives licence and may be reused according to the conditions of the licence. For more details see: http://creativecommons.org/licenses/by-nc-nd/2.5/ A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription.For more information, please contact eprints@nottingham.ac.uk shape, position, margination and internal architecture of the testes (England, 58 1991;Eilts et al., 1993; Paltiel et al., 2002;Gouletsou et al., 2008; Souza et al., 59 2014) and prostate gland (Blum et al., 1985; Juniewicz et al., 1989; England, 60 1991;Eilts et al., 1993; Ruel et al., 1998; Paltiel et al., 2002; Gouletsou et al., 61 2008; Freitas et al., 2013;Freitas et al., 2015). Ultrasonography also provides a 62 valuable tool in assessing reproductive pathology (Cartee and Rowles, 1983; 63 Feeney et al., 1987; Pugh and Konde, 1991; Cooney et al., 1992; England, 1995; 64 Keenan, 1998;Nautrup and Tobias, 2001; Hecht, 2008). 65 66In clinical practice, ultrasound images are subjectively assessed and described in 67 terms of their image texture; principally echogenicity and heterogeneity. A small 68 number of studies have proposed a relationship between grossly detectable 69 lesions within the testes and semen quality (England, 1991; Vencato et al., 2014). 70Objective analysis of echogenicity from measurements of pixel intensity is 71 however possible using digital image analysis (Ivancic and Mai, 2008) The aim of this study was to measure testicular and prostatic ultrasonographic 89 echogenicity and heterogeneity using digital image analysis, and investigate the 90 relationships between these measures and semen quality in a group of known 91 fertile dogs. 92 93 Materials and Methods 94 Study animals 95Forty-three stud dogs (21 Labrador Retrievers, 12 Golden Retrievers, 6 German 96 Shepherds, 1 Border Collie, 1 Flat Coated Retriever, 1 Irish Water Spaniel and 1 97Standard Poodle) with a mean weight of 35.5 ± 5.8 kg (range 20.6 to 54.1 kg) 98 aged between 1.1 and 9.3 years (mean 4.2 ± 2.0 years) were examined. Dogs 99 5 were selected on the basis that they met the following inclusion criteria; (1) 100 clinically healthy, (2) o...

BackgroundMeasurement of testicular artery blood flow is used in several species to evaluate reproductive function and testicular and scrotal pathology. In dogs there are inconsistent reports about normal flow in post-pubertal dogs and no information concerning pre-pubertal dogs. The aim of this study was to describe regional differences in testicular artery blood flow in clinically normal post-pubertal and pre-pubertal dogs with no history of reproductive tract disease.ResultsThe post-pubertal dogs produced normal ejaculates throughout the study. In all dogs the three different regions of the artery were imaged and monophasic flow with an obvious systolic peak and flow throughout diastole was observed on every occasion. The highest peak systolic velocity (PSV) and end diastolic velocity (EDV) were measured within the distal supra-testicular artery and marginal artery whilst the lowest PSV and EDV were measured within the intra-testicular arteries. Flow measurements were not different between left and right testes and were consistent between dogs on different examination days. Calculated resistance index (RI) and pulsatility index (PI) were lowest in the intra-testicular arteries.The pre-pubertal dogs had significantly smaller testes than the post-pubertal dogs (p < 0.05) and were unable to ejaculate during the study. The three different artery regions were imaged at every examination time point, and flow profiles had a similar appearance to those of the post-pubertal dogs. PSV, EDV, RI and PI showed a similar trend to the post-pubertal dogs in that values were lowest in the intra-testicular arteries. Notably, values of PSV, EDV, RI and PI were significantly lower (p < 0.05) in pre-pubertal dogs compared with post-pubertal dogs.ConclusionsThis study demonstrated important regional and pubertal differences in testicular artery blood flow of dogs, and form the basis for establishing baseline reference values that may be employed for the purposes of clinical diagnosis.

ContentsThe reproductive potential of male animals is commonly evaluated using a breeding soundness examination incorporating B-mode ultrasound examination of the testes and recently Doppler ultrasound examination of the testicular arteries. These techniques may detect testicular normality or pathology, and while some measured parameters are associated with semen quality at the time of ultrasound examination, few studies have investigated the relationship with future semen quality. We hypothesized that B-mode and Doppler ultrasound measurements would correlate with future semen quality. Within two studies, we investigated the relationship between ultrasound measured testicular volume, testicular echogenicity, testicular homogeneity, subjective assessment of the testicular parenchyma, testicular artery resistance index, and pulsatility index with subsequent semen quality. Fifty-five normal fertile dogs of which 29 had stable semen quality and 26 had a subsequent decline in semen quality were examined during a 6-month period commencing 62 days after the ultrasound examination.Statistical analysis showed that no ultrasound parameters were predictive of future total sperm output or percentage live normal sperm. However, mean testicular echogenicity was positively related to future sperm motility (t = 2.202, p = .039). We conclude that quantitative ultrasound assessment of the appearance of the testicular parenchyma has potential for the evaluation of future semen quality in dogs.

Companion Animals Ultrasonographic imaging is an important diagnostic tool because it allows assessment of the shape, size, position, margination and internal architecture of organs, as well as facilitating the study of vascular supply and vascularisation. Recently, there has been considerable development of B-mode, Doppler and contrast-enhanced ultrasonography for examination of the reproductive tract of dogs, both for studying normal physiology and in the clinical setting. This article describes the practical examination of the canine prostate gland and testes using a variety of ultrasound techniques, and details the normal appearance and blood flow of these organs as well as changes that may be observed with common reproductive disorders. Imaging the prostate gland An image of the prostate gland can be achieved by placing the ultrasound transducer on the caudoventral abdomen, adjacent to the prepuce. Most dogs can be examined without sedation while standing or when positioned in dorsal or lateral recumbency. A 7.5 to 10.0 MHz transducer is preferable and imaging is facilitated by the presence of urine within the bladder. Imaging should always be undertaken in both the transverse and longitudinal planes, while the frontal (dorsal) plane is also useful. When the prostate lies entirely within the pelvis, transrectal imaging can be performed in large dogs but not in small dogs. B-mode ultrasonography Normal prostate gland The prostate is an ovoid-shaped, bilobed gland positioned

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