A coloração pela prata das regiões organizadoras de nucléolos (NORs) é caracterizada por marcar proteínas ligadas ao ácido ribonucleico ribossômico, avaliando a proliferação em células normais ou neoplásicas. Objetivou-se estudar, em testículos de ovinos obtidos em matadouro, a validade do uso da técnica de coloração pela prata (AgNOR) na identificação das regiões organizadoras de nucléolo (NORs) em células saudáveis da linhagem espermatogênica. Utilizaram-se 43 pares de testículos de ovinos mestiços entre seis e 10 meses de idade. Testes de Wilcoxon e Spearman foram empregados, com nível de 5%. As médias das NORs nas células das gônadas direita e esquerda foram, respectivamente: espermatogônia (8,77±1,14 e 9,04±0,96), espermatócitos (4,99±2,00 e 6,20±2,07; P<0,05), Leydig (8,05±2,82 e 7,89±2,29) e Sertoli (8,07±1,88 e 7,61±2,16; P<0,05). Houve correlação (P<0,05) entre os lados para o número de NORs: espermatócitos x Leydig (0,49); espermatócitos x Sertoli (0,49) e Leydig x Sertoli (0,96). Conclui-se ser válido o emprego da técnica AgNOR para avaliar o potencial proliferativo das células saudáveis em testículos de ovinos com prática execução e baixo custo. (8.77±1.14 and 9.04±0.96), spermatocytes (4.99±2.00 and 6.20±2.07, P<0.05), Leydig (8.05±2.82 and 7.89±2.29) and Sertoli cells (8.07±1.88 and 7.61±2.16; P<0.05
The AgNOR staining technique for nucleolar organizer regions (NORs) is characterized by marking proteins related to ribossomal ribonucleic acid evaluating celular proliferation. The objective was to study the ovarian morphometry and the value of AgNOR thecnique application to evaluate the proliferation of healthy cels from ovarian structures in crossbred ewe. 102 pairs of ovaries were collected and from the largest follicle of each ovary was measured its diameter. From the largest corpus luteum (CL) was measured the diameter and width, classifying the CL in: included, protruberant, massive and cavity, followed by AgNOR staining. The significance level was 5%. It had difference (p<0.05) for the diameter of the largest follicle between right (RO): 4.2±2,3mm and left (LO): 3.6±2,2mm sides. There was a correlation (p<0.01) between the NORs of internal theca (IT) and external theca (ET): RO (0.46) and LO (0.61); NORs of granulosa and IT: RO (0.54) and LO (0.59); and NORs of granulosa and ET: RO (0.34) and LO (0.38). Was conclude that the NORs quantification was appropriate to study the determination of the potential for ovarian structures proliferation, which was similar for right and left sides.
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