The Effects of Different Media, Sucrose Concentrations and Natural Additives on Plantlet Growth of Phalaenopsis Hybrid 'Pink'

Zahara, Meutia; Datta, Avishek; Boonkorkaew, Patchareeya; Mishra, Abha

doi:10.1590/1678-4324-2017160149

Cited by 32 publications

(22 citation statements)

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“…Palavras-chave: orquídea, protocormoides, embriogênese somática. of seedlings propagated by vegetative means are not uniform; therefore, propagation through tissue culture is desirable (ZAHARA et al, 2017). They are monopodial, alternifolia plants showing vegetative meristem finishing.…”

Section: Resumo: O Presente Estudo Buscou Induzir a Formação De Embrimentioning

confidence: 99%

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Somatic embryos from Phalaenopsis classic Spotted Pink (Orchidaceae) protocorms

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The aim of the present study was to induce the formation of somatic embryos in protocorms from Phalaenopsis Classic Spotted Pink hybrids at two physiological maturation stages, namely: 80 and 120 days after seed inoculation (DASI). Protocorms were inoculated in ½ MS medium supplemented with 0.1 mg L-1 ANA and 3 mg L-1 TDZ. Protocorms were inoculated 120 days after sowing were more developed at the 15th cultivation day due to the formation of pro-embryogenic structures. It was possible seeing the formation of globular- and torpedo-stage somatic embryos at the 30th day of cultivation in somatic embryogenesis (SE) induction medium. The protocorms inoculated at the 80th DASI did not formed somatic embryos; they oxidized 20 days after cultivation in SE-induction medium. The formation of somatic embryos happened directly on the explant, thus characterizing a direct somatic embryogenesis. The embryos converted into plants when the somatic embryos were transferred to the nutrient medium containing no growth regulator. Therefore, it was concluded that the somatic embryos induction from protocorms 120 days after sowing was positive, since the embryos were able to become plants and presented vegetative organs with morphological traits similar to those of the matrix plant.

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Section: Resumo: O Presente Estudo Buscou Induzir a Formação De Embrimentioning

confidence: 99%

“…Phalaenopsis orchids are recognized as the most popular orchid genus in the horticultural industry due to their large, colorful, and durable flowers as well as their wider adaptability to room conditions (ZAHARA et al, 2017). This species has been widely investigated in the last decade due to the high demand for cut or pot flowers (PARK et al, 2015).…”

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Somatic embryos from Phalaenopsis classic Spotted Pink (Orchidaceae) protocorms

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“…Kultur jaringan Phalaenopsis hybrid 'Pink' menggunakan segmen daun dapat memunculkan kalus dan juga plantlet dalam kurun waktu 8 minggu dengan 60% survival rate, eksplan ini dikulturkan pada media ½MS dengan kombinasi 0 mg/l NAA dan 2 mg/l TDZ. Sementara itu, eksplan daun yang dikulturkan pada media ½MS dengan kombinasi 0 mg/l NAA dan 3 mg/l BAP sukses membentuk plantlet dalam kurun waktu 10 minggu setelah inkubasi dengan survival rate mencapai 70% [23]. Masalah yang berkaitan dengan pencoklatan eksplan daun dan nekrosis adalah salah satu kendala yang banyak dilaporkan dalam beberapa publikasi [19,23].…”

Section: Teknik Kultur Jaringan Tanaman Anggrekunclassified

“…Sementara itu, eksplan daun yang dikulturkan pada media ½MS dengan kombinasi 0 mg/l NAA dan 3 mg/l BAP sukses membentuk plantlet dalam kurun waktu 10 minggu setelah inkubasi dengan survival rate mencapai 70% [23]. Masalah yang berkaitan dengan pencoklatan eksplan daun dan nekrosis adalah salah satu kendala yang banyak dilaporkan dalam beberapa publikasi [19,23]. Kalus, PLBs dan bahkan plantlet sempat terbentuk, namun dalam jangka waktu yang singkat bisa mengalami kematian.…”

Section: Teknik Kultur Jaringan Tanaman Anggrekunclassified

A REVIEW: MICROPROPAGATION OF PHALAENOPSIS sp FROM LEAF AND FLOWER STALK EXPLANTS

Zahara¹

2017

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Phalaenopsis orchids are recognized as the most popular orchid genus in the world, especially in horticultural industry due to their large, colorful, and durable flowers as well as their wider adaptability to room conditions. The characteristics of seedling propagated by vegetative means are not uniform; therefore, propagation through tissue culture is desirable. Although the micro propagation of Phalaenopsis has shown very good development, but the wide spread of micro propagation still limited due some problems such as the exudation of phenolic compounds, the PGR concentration, the media used, somaclonal variation, the chosen explants, etc. This paper endeavor to include some important investigations based on the common explants used; leaf and flower stalk. Keywords: Micropropagation, Phalaenopsis, leaf explant, flower stalk ReferencesAnonymous. Orchid (Orchidaceae). Diakes tanggal 13 Januari 2013 dari http://www.rainforest-alliance.org/kids/species-profiles/orchid. Rainforest Alliance. 2002.Pillon, Y.; Chase, M. W.Taxonomic exaggeration and its effects on orchid conservation. Conservation Biology. 2007, 21, 263–265.Thengane, S. R.; Deodhar, S. R.; Bhosle, S. V.; Rawal, S. K. Direct somatic embryogenesis and plant regenaration in Garciniaindica Chois’. Current Science. 2006, 91(8), 1074-1078.Yuswanti, H.; Dharma, I. P.; Utama. ; Wiraatmaja, I. W. Mikropropagasi anggrek Phalaenopsis dengan menggunakan eksplan tangkai bunga. AGROTROP. 2015, 5(2): 161-166.Raynalta, E.; Sukma, D. Pengaruh komposisi media dalam perbanyakan protocorm like bodies, pertumbuhan plantlet, dan aklimatisasi Phalaenopsis amabilis. J. Hort. Indonesia. 2013, 4(3): 131-139.Kosir, P.; Skof, S.; Luthar, Z. Direct Shoot Regeneration from Nodes of Phalaenopsis of Orchids. Acta Agriculturae Slovenica. 2004, 83, 233–242.Arditti, J. R. ; Ernst. Micropropagation of Orchids. Wiley-Interscience. New York, 1993.Park, Y. S.;Kakuta, S.; Kano, A.; Okabe, M.Efficient propagation of protocorm-like bodies of Phalaenopsis in liquid medium. Plant Cell, Tissue and Organ Culture. 1996, 45, 79–85.Park, S. Y. ; Yeung, E. C.; Chakrabarty, D. ; Paek, K. Y. An efficient direct induction of protocorm-like bodies from leaf subepidermal cells of Doritaenopsis hybrid using thin-section culture. Plant Cell Reports. 2002, 21, 46–51.Zahara, M.; Datta, A.; Boonkorkaew, P. Effects of sucrose, carrot juice and culture media on growth and net CO2 exchange rate in Phalaenopsis hybrid ‘Pink’. ScientiaHorticulturae. 2016,205, 17–24.Hee, K. H.; Loh, C. S.; Yeoh, H. H. In vitro flowering and rapid in vitro embryo production in Dendrobium Chao Praya Smile (Orchidaceae). Plant Cell Reports. 2007, 26, 2055–2062.Kannan, N. An in vitro study on micropropagation of Cymbidium orchids. Current Biotica. 2009, 3, 244–250.Steward, Jr. N. C. Plant Biotechnology and Genetics. Willey, A john Willey & Sons, INC., Publication. 2008.George, E. F.; Sherington, P. D.Biotechnology by tissue culture. Exegetics Ltd. 1994.Nursyamsi. Teknik kultur jaringan sebagai alternatif perbanyakan tanaman untuk mendukung rehabilitasi lahan. Makalah pada ekspose hasil-hasil penelitian balai penelitian kehutanan makasar. Makasar, 2010.Aditi, J. F. L. S.; Krikorian, A. D. Orchid mircropropagation: the path from laboratory to commercialization and an account of several unappreciated investigators. Botanical Journal of of the Linnean Society. 1996, 122: 183-241.Gunawan, L. W. Teknik Kultur Jaringan Tanaman. Pusat Antar Universitas (PAU) Bioteknologi IPB. 1998. Bogor.Chugh, S. Guha, S.; Rao, I. U. Micropropagation of orchids: A review on the potential of different explants. Scientia Horticulturae. 2009, 122, 507–520.Ramdan. Kultur daun dan pangkal batang in vitro anggrek bulan raksasa (Phalaenopsis gigantea J.J.Smith) pada beberapa media kultur jaringan. Departemen agronomi dan hortikultura, Fakultas pertanian IPB. 2011.Latip, M. A. R.; Murdad, Z. A.; Aziz, L. H.; Ting, L. M.; Govindasamy.; R. Pipin. Effects of N6-Benzyladenine and Thidiazuron on Poliferation of Phalaenopsis gigantea Protocorm. AsPac J. Mol. Biol. Biotechnol. 2010, 18(1): 217-220 p.Niknejad, A.; Kadir, M. A.; Kadzimin, B. S. In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroidaceae: Orchidaceae). African Journal of Biotechnology.2010, 10, 11808–11816.Chen, J. T.; Chang, W. C. Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis. Biologia Plantarum. 2006, 50, 169–173.Zahara, M. Disertasi doktor: The Effects of Plant Growth Regulators and Natural Additives on Direct Shoot Regeneration and Plantlet Growth of Phalaenopsis hybrid ‘Pink’. Asian Institute of Technology, Pathumthani. Thailand. 2016.Xu, C. J.; Li, H.; Zhang, M. G. Preliminary studies on the elements of browning and the changes in cellular texture of leaf explant browning in Phalaenopsis. Acta Horticulturae Sinica. 2005, 32, 1111–1113.Tokuhara, K; Mii, M. Induction of embryonic callus and cell suspension culture from shoot tips excised from flower stalk buds of Phalaenopsis (Orchidaceae). In Vitro Cellular & Developmental Biology–Plant. 2001, 37, 457–461Balilashaki, K.; Naderi, R.; Kalantari, S.; Soorni, A. Mircropropagation of Phalaenopsis amabilis cv Cool ‘Breeze’ with using flower stakl nodes and leaves of sterile obtained from node cultures. IJFAS, 2014.Semiarti, E.; Indrianto, A.; Purwanto, A. Agrobacterium-Mediated transformation of Indonesian orchids for micropropagation, genetic transformation, Prof. MarÃa Alvarez (Ed.), ISBN: 978-953-307-364-4, InTech, 2011. Available from: http://www.intechopen.com/books/ genetic-transformation/agrobacterium-mediated-transformation-ofindonesian-orchids-for-micropropagation.

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“…As orquídeas estão se destacando nesse setor, devido a sua capacidade de combinação genética, beleza, diversidade de cor, forma e durabilidade de suas flores (Zahara et al, 2017). Assim, os produtores têm respondido atentamente ao crescimento contínuo deste seguimento no mercado interno, e a cada ano vêm introduzindo novos híbridos de orquídeas, resultantes de melhoramentos, sendo registrados atualmente 2.345 cultivares dessas plantas (Junqueira & Peetz, 2017b).…”

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Influência da luz, ventilação natural e tamanho do frasco no crescimento e desenvolvimento de denphal (Orchidaceae)

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et al. 2019

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RESUMO: Objetivou-se avaliar o crescimento da orquídea denphal, em função da luz, sistema de micropropagação e tamanho dos frascos de cultivo. O delineamento experimental utilizado foi inteiramente casualizado e os tratamentos arranjados em esquema fatorial 2 x 2 x 2 com cinco repetições. Plântulas cultivadas in vitro por 90 dias, a partir do início do período experimental, foram retiradas dos frascos, lavadas e avaliadas quanto ao número de folhas, altura da plântula, diâmetro do maior pseudobulbo, número de brotos e raízes, comprimento da maior raiz e da maior folha, e massa fresca. A seguir foram plantadas em substrato (esfagno rosa + fibra de coco) e transferidas para ambiente ex vitro. Com o intuito de investigar seus incrementos, após 90 dias, as plantas foram avaliadas quanto às mesmas características iniciais. Os diferentes espectros de luz não foram determinantes para o crescimento da planta. A utilização do sistema de ventilação natural em frascos de maior tamanho foi o fator que proporcionou condições favoráveis na promoção do crescimento, tanto no cultivo in vitro quanto no ex vitro do denphal.

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The Effects of Different Media, Sucrose Concentrations and Natural Additives on Plantlet Growth of Phalaenopsis Hybrid 'Pink'

Abstract: ABSTRACT

Cited by 32 publications

References 38 publications

Somatic embryos from Phalaenopsis classic Spotted Pink (Orchidaceae) protocorms

Somatic embryos from Phalaenopsis classic Spotted Pink (Orchidaceae) protocorms

A REVIEW: MICROPROPAGATION OF PHALAENOPSIS sp FROM LEAF AND FLOWER STALK EXPLANTS

Influência da luz, ventilação natural e tamanho do frasco no crescimento e desenvolvimento de denphal (Orchidaceae)

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