Somatic embryos from Phalaenopsis classic Spotted Pink (Orchidaceae) protocorms

Abstract

The aim of the present study was to induce the formation of somatic embryos in protocorms from Phalaenopsis Classic Spotted Pink hybrids at two physiological maturation stages, namely: 80 and 120 days after seed inoculation (DASI). Protocorms were inoculated in ½ MS medium supplemented with 0.1 mg L-1 ANA and 3 mg L-1 TDZ. Protocorms were inoculated 120 days after sowing were more developed at the 15th cultivation day due to the formation of pro-embryogenic structures. It was possible seeing the formation of globular- and torpedo-stage somatic embryos at the 30th day of cultivation in somatic embryogenesis (SE) induction medium. The protocorms inoculated at the 80th DASI did not formed somatic embryos; they oxidized 20 days after cultivation in SE-induction medium. The formation of somatic embryos happened directly on the explant, thus characterizing a direct somatic embryogenesis. The embryos converted into plants when the somatic embryos were transferred to the nutrient medium containing no growth regulator. Therefore, it was concluded that the somatic embryos induction from protocorms 120 days after sowing was positive, since the embryos were able to become plants and presented vegetative organs with morphological traits similar to those of the matrix plant.

Somatic embryogenesis of the selected intergeneric hybrid between Phalaenopsis 2166 and Vanda ‘Saint Valentine’: Application of NAA and TDZ

“…Since the 30 th day after incubation, the explants of the selected intergeneric hybrid of Phalaenopsis 2166 x Vanda' Saint Valentine' were subjected to 12 hour-exposure to light and another 12 hours in the dark. As a comparison, the explants of Phalaenopsis Classic spotted pink started to form the last globular structure on the 23 rd day, and the embryos began to form coleoptelar (Pereira et al 2019). Meanwhile, direct somatic embryogenesis from leaf explants in Phalaenopsis' Little Steve' revealed that somatic embryos were formed on the 30 th day after explant incubation in the dark (Kuo et al 2005).…”