Influence of Benzyladenine and Thidiazuron on Shoot Regeneration from Leaf and Shoot tip Explants of Sedum sarmentosum Bunge

Abstract

Effects of N 6 -benzyladenine (BA) or thidiazuron (TDZ) on adventitious shoot regeneration and axillary shoot multiplication of Sedum sarmentosum was investigated. Leaf and shoot tip explants obtained from in vitro-grown shoots of S. sarmentosum were cultured on Murashige and Skoog (MS) medium supplemented with 0, 2.0, 4.0 or 8.0 µM BA or TDZ. Of the two cytokinins studied, BA was found to be more responsive as compared to TDZ with respect to number of shoots produced per explant. High frequency of shoot regeneration (92.2%) with a mean of 12.3 shoots was produced when the leaf explants were cultured on MS medium supplemented with 8.0 µM BA. The highest number of shoots (25.4) was obtained when shoot tip explants were cultured on MS medium devoid of cytokinins after 35 days of culture. For root induction, regenerated shoots were cultured on MS medium supplemented with 0, 2.0, 4.0 or 8.0 µM indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or α-naphthaleneacetic acid (NAA). The highest number of (17.6) roots per shoot was obtained on MS medium supplemented with 2.0 µM IBA after 30 days of culture. Regenerated plantlets were successfully acclimatized in the greenhouse with 100% survival rate.

Micropropagation and Subsequent Enrichment of Carotenoids, Fatty Acids, and Tocopherol Contents in Sedum dasyphyllum L

“…Our results well correlate to the existing knowledge, that BA has been observed to be the most frequently used cytokinin for shoot induction in Sedum acre, S. aizoon, S. gracile, S. floriferum and S. spectabile (Wojciechowicz, 2007 , 2009 ), S. alfredii and S. plumbizincicola (Zhao et al, 2009 ; Liu et al, 2017 ), S. drymarioides (Kitamura et al, 2002 ), S. erythrostichum (Yoon et al, 2002 ), and S. telephium (Brandao and Salema, 1977 ). Inhibitory effects at higher concentrations of TDZ on shoot production have also been reported earlier in S. sarmentosum (Kim and Sivanesan, 2016 ) and S. spectabile (Yang et al, 2012 ).…”

“…Similarly, the addition of phytohormones to MS medium is reported to increase shoot production in S. alfredii (Liu et al, 2017 ). On the contrary, our previous reports have shown that the inclusion of BA or TDZ to MS medium did not influence shoot numbers in S. sarmentosum (Kim and Sivanesan, 2016 ). Thus, it appears that phytohormone requirements for ideal axillary shoot multiplication is species dependent and varies between different Sedum species.…”

“…Micropropagation is an efficient technique for mass production of reputed ornamental and medicinal plants (Jeong and Sivanesan, 2016 ; Kim and Sivanesan, 2016 ; Kwaśniewska and Pawłowska, 2017 ). Shoot tips and stem nodes are frequently used as explants for the multiplication of true-to-type plants.…”

“…Successful in vitro rooting of shoots frequently depends on plant species, culture medium strength, and auxin treatment. Auxin is usually added to the culture medium to promote root induction in regenerated shoots of S. alfredii (Zhao et al, 2009 ) and S. sarmentosum (Kim and Sivanesan, 2016 ). However, in vitro -raised shoots of Sedum acre, S. aizoon, S. gracile, S. floriferum , and S. spectabile were rooted on auxin-free medium (Wojciechowicz, 2007 ).…”

“…However, low proliferation rate and the shortage of plant materials often affected their mass cultivation. Thus, in vitro propagation methods have been developed in several Sedum species for mass multiplication and plant improvement (Brandao and Salema, 1977 ; Kitamura et al, 2002 ; Yoon et al, 2002 ; Wojciechowicz, 2007 , 2009 ; Zhao et al, 2009 ; Yang et al, 2012 ; Kim and Sivanesan, 2016 ; Liu et al, 2017 ). In these studies, leaves, nodes, petals, shoot tips and stem explants obtained from greenhouse- or field- grown plants of Sedum acre, S. aizoon, S. gracile, S. floriferum, S. alfredii, S. drymarioides, S. erythrostichum, S. plumbizincicola, S. sarmentosum, S. spectabile , and S. telephium were used for shoot or plant regeneration.…”