In vitro propagation of a rare orchid, Bulbophyllum nipondhii was carried out. Five different media were tested to find the suitable medium for seed germination and seedling development. The tangible results were obtained on VW medium. To assess the effect of pollination types on seed germination and seedlings development, seeds derived from different self-, cross-and open-pollination were examined. Open-pollinated seeds produced the best germination and the highest seedling development, followed by cross-and self-pollination. VW medium was supplemented with 0, 25, 50 or 75 g/l potato extract (PE) and 0, 50, 100, 150 or 200 ml/l coconut water (CW) to identify their most suitable concentration. PE (75 g/l) with 100 ml/l CW was found to be best combination.
The panda plant (Kalanchoe tomentosa Baker) is a popular ornamental succulent. The optimal method for in vitro plantlet propagation was investigated. Effects of plant growth regulator on growth and development of leaf explants were determined using various concentrations of Thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) at 0, 0.01, 0.1, 0.2 and 0.5 mg L-1. A concentration of 0.01 mg L-1 2,4-D gave the highest plantlet quality derived from direct organogenesis. Higher concentrations of 2,4-D promoted callus proliferation. The lowest concentration of 0.01 mg L-1 TDZ induced shorter shoots, while higher concentrations resulted in greater callogenesis and inhibit root production. After in vitro culture, plantlets from the optimal treatment were acclimatized by exposure to growth in sand, sand with coconut husk (2:1), sand with potting soil (2:1) and sand with perlite (2:1). Highest survival percentage (100%) was found in plantlets grown in sandy soil, the most well-drained material of those selected materials. Results demonstrated an alternative production method for panda plantlets using plant tissue culture techniques.
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