TDZ and 2,4-D on in vitro propagation of panda plant from leaf explants

Abstract

The panda plant (Kalanchoe tomentosa Baker) is a popular ornamental succulent. The optimal method for in vitro plantlet propagation was investigated. Effects of plant growth regulator on growth and development of leaf explants were determined using various concentrations of Thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) at 0, 0.01, 0.1, 0.2 and 0.5 mg L-1. A concentration of 0.01 mg L-1 2,4-D gave the highest plantlet quality derived from direct organogenesis. Higher concentrations of 2,4-D promoted callus proliferation. The lowest concentration of 0.01 mg L-1 TDZ induced shorter shoots, while higher concentrations resulted in greater callogenesis and inhibit root production. After in vitro culture, plantlets from the optimal treatment were acclimatized by exposure to growth in sand, sand with coconut husk (2:1), sand with potting soil (2:1) and sand with perlite (2:1). Highest survival percentage (100%) was found in plantlets grown in sandy soil, the most well-drained material of those selected materials. Results demonstrated an alternative production method for panda plantlets using plant tissue culture techniques.

Photosynthetic apparatus performance and anatomical modulations of Alcantarea imperialis (Bromeliaceae) exposed to selenium during in vitro growth

“…In vitro techniques are often used for large-scale propagation of plants with high economic value or endangered status (Manokari et al 2020, Kaur et al 2021, Shekhawat et al 2021). These techniques are particularly applied to ornamental species, including members of the Bromeliaceae family (Martins et al 2015(Martins et al , 2018Lando et al 2016, Martínez-Estrada et al 2019, Pakum et al 2021. Alcantarea imperialis (Carrière) Harms is among the bromeliad species with ornamental interest.…”