Micropropagation and Subsequent Enrichment of Carotenoids, Fatty Acids, and Tocopherol Contents in Sedum dasyphyllum L

Park, Han Yong; Saini, Ramesh Kumar; Gopal, Judy; Keum, Young‐Soo; Kim, Doo Hwan; Lee, Onew; Sivanesan, Iyyakkannu

doi:10.3389/fchem.2017.00077

Cited by 8 publications

(10 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To our knowledge, the carotenoid composition and total carotenoid contents in the organs of M. maritima have not been previously reported. All- E -lutein was the major carotenoid in leaf tissues (from in vitro -raised shoots on MS + 4 µM TDZ + 1 µM NAA) of M. maritima and a similar result has been reported for the leaves of Ajuga multiflora [3] and Aronia melanocarpa [23] and the shoots of Sedum dasyphyllum [32]. All- E -lutein has also been identified as the predominant carotenoid in the leaf extracts of another member of the Boraginaceae, Cordia myxa [33].…”

Section: Discussionsupporting

confidence: 75%

Micropropagation and Quantification of Bioactive Compounds in Mertensia maritima (L.) Gray

Park

Kim

Saini

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

The goal of this study was to establish an efficient protocol for the large-scale propagation of Mertensia maritima (L.) Gray, and evaluate the carotenoid, fatty acid, and tocopherol contents in the leaves of in vitro regenerated shoots. Surface-disinfected node and shoot tip explants were placed on semisolid Murashige and Skoog (MS) medium with 0–16 µM N6-benzyladenine (BA), kinetin, (KN), and thidiazuron (TDZ) alone, or in combination with, 1 or 2 µM α-naphthaleneacetic acid (NAA). Of the three different cytokinins employed, TDZ elicited the best results for axillary shoot proliferation. A maximum frequency of shoot initiation above 84%, with a mean of 8.9 and 4.8 shoots per node and shoot tip, respectively, was achieved on the culture medium supplemented with 4 µM TDZ. A combination of TDZ + NAA significantly increased the percentage of multiple shoot formation and number of shoots per explant. The best shoot induction response occurred on MS medium with 4 µM TDZ and 1 µM NAA. On this medium, the node (93.8%) and shoot tip (95.9%) explants produced an average of 17.7 and 8.6 shoots, respectively. The highest root induction frequency (97.4%) and number of roots per shoot (25.4), as well as the greatest root length (4.2 cm), were obtained on half-strength MS medium supplemented with 4 µM indole-3-butyric acid (IBA). The presence of six carotenoids and α-tocopherol in the leaf tissues of M. maritima was confirmed by HPLC. Gas chromatography-mass spectrometry analysis confirmed the presence of 10 fatty acids, including γ-linolenic acid and stearidonic acid in the leaf tissues of M. maritima. All-E-lutein (18.49 μg g−1 fresh weight, FW), α-tocopherol (3.82 μg g−1 FW) and α-linolenic acid (30.37%) were found to be the significant compounds in M. maritima. For the first time, a successful protocol has been established for the mass propagation of M. maritima with promising prospects for harnessing its bioactive reserves.

show abstract

Section: Discussionsupporting

confidence: 75%

Micropropagation and Quantification of Bioactive Compounds in Mertensia maritima (L.) Gray

Park

Kim

Saini

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, the ex-vitro-raised KP-BG leaves had higher total carotenoid content (93.84 µg/g FW) than the in-vitro-grown KP-BG leaves (80.40 µg/g FW). A similar result was observed in Aronia melanocarpa [45] and Sedum dasyphyllum [46]. Of the identified carotenoids, Lut was found to be a major component (44.38 µg/g FW) in KP-BG leaves (Table 1).…”

Section: Discussionsupporting

confidence: 80%

“…Of the identified carotenoids, Lut was found to be a major component (44.38 µg/g FW) in KP-BG leaves (Table 1). Lut has been identified as the predominant carotenoid in leafy green vegetables and the mature leaves of many medicinal plants [37,39,[45][46][47].…”

Section: Discussionmentioning

confidence: 99%

“…α-Linolenic acid was found (39.68-43.12%) to be the most abundant fatty acid in KP-BG leaves (Table 2). Similarly, α-Linolenic acid was identified as the predominant fatty acid in the leaves of Mertensia maritima [39], Aronia melanocarpa [45], Sedum dasyphyllum [46], and Ajuga multiflora [60]. In the in-vitro-raised KP-BG leaves, the proportion of oleic acid (OA) decreased, and that of linoleic acid (LA) increased.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Lipophilic Antioxidants in the Leaves of Kaempferia parviflora Wall. Ex Baker Using LC–MRM–MS and GC–FID/MS

et al. 2021

Self Cite

View full text Add to dashboard Cite

Lipophilic metabolites such as carotenoids, fatty acids, vitamin K1, phytosterols, and tocopherols are important antioxidants that are used in the cosmetics, foods, and nutraceutical industries. Recently, there has been a growing demand for the use of byproducts (wastes) as a potential source of industrially important compounds. The leaves of Kaempferia parviflora (black ginger) (KP-BG) are major byproducts of KP-BG cultivation and have been reported to contain several bioactive metabolites; however, the composition of lipophilic metabolites in KP-BG leaves has not been examined. In this study, the lipophilic antioxidant profile was analyzed in the leaves of KP-BG plants grown in vitro and ex vitro. Lipophilic compounds, namely carotenoids (80.40–93.84 µg/g fresh weight (FW)), tocopherols (42.23–46.22 µg/g FW), phytosterols (37.69–44.40 µg/g FW), and vitamin K1 (7.25–7.31 µg/g FW), were quantified using LC–MRM–MS. The fatty acid profile of the KP-BG leaves was identified using GC–FID/MS. The content of individual lipophilic compounds varied among the KP-BG leaves. Ex vitro KP-BG leaves had high levels of lutein (44.38 µg/g FW), α-carotene (14.79 µg/g FW), neoxanthin (12.30 µg/g FW), β-carotene (11.33 µg/g FW), violaxanthin (11.03 µg/g FW), α-tocopherol (39.70 µg/g FW), α-linolenic acid (43.12%), palmitic acid (23.78%), oleic acid (12.28%), palmitoleic acid (3.64%), total carotenoids (93.84 µg/g FW), and tocopherols (46.22 µg/g FW) compared with in vitro KP-BG leaves. These results indicate that ex-vitro-grown KP-BG leaves could be used as a valuable natural source for extracting important lipophilic antioxidants.

show abstract

“…Therefore, the regulation of the biosynthesis of oil and fatty acids in Castor bean with phytohormones supplemented for the harvest of more high-value products like oil and fatty acids are promising. There are several reports on the substantial in uence of plant growth regulators such as auxin (2,4-D) and cytokinin (BA) on plant lipid composition and lipid metabolism in terms of fatty acids induction and alteration of their composition speci cally the alterations of both the ratios of C18:2 to C18:1 and C18:3 to C18:2 [55][56]…”

Section: Discussionmentioning

confidence: 99%

The Effects of Plant Growth Regulators On The Oil Content And Fatty Acid Composition of Ricinus Communis L.: An In Vitro Study

Ergün

2021

Preprint

View full text Add to dashboard Cite

This study aimed to investigate the effects of plant growth regulators in callus cell culture on the oil content and fatty acid composition of the castor bean (Ricinus communis L.). Ricinus communis L. are valued for its oil and the performance of oil is closely related to its fatty acid composition. Thus, producing oil in vitro with favored fatty acid profiles is a promising research area and may also offer industrial opportunities. In line with this, the total amount of fat and the fatty acid composition of the samples, which were endosperm and calli obtained by treatment of various doses of plant growth regulators were determined. Results showed that the type and amount of the plant growth regulator used in the media affect the fatty acid composition. In detail, the biggest change was shown by Indole-3-Acetic Acid (IAA), in general, using the plant growth regulators at 5 mgL-1, instead of 20 mgL-1, was found to have induced larger differentiations. The effect of a natural plant growth regulator (IAA) on fatty acid profiles was larger than the synthetic ones (NAA, 1-Naphthaleneacetic acid, and 2,4 D, 2,4-Dichlorophenoxyacetic acid). The media containing 5 mgL-1 of NAA, 20 mgL-1 of NAA, 20 mgL-1 of 2,4 D, or 5 mgL-1 of 2,4 D gave similar results.

show abstract

Micropropagation and Subsequent Enrichment of Carotenoids, Fatty Acids, and Tocopherol Contents in Sedum dasyphyllum L

Cited by 8 publications

References 53 publications

Micropropagation and Quantification of Bioactive Compounds in Mertensia maritima (L.) Gray

Micropropagation and Quantification of Bioactive Compounds in Mertensia maritima (L.) Gray

Analysis of Lipophilic Antioxidants in the Leaves of Kaempferia parviflora Wall. Ex Baker Using LC–MRM–MS and GC–FID/MS

The Effects of Plant Growth Regulators On The Oil Content And Fatty Acid Composition of Ricinus Communis L.: An In Vitro Study

Contact Info

Product

Resources

About