“…Also, Rathnavelu, Alitheen, Sohila, Kanagesan, and Ramesh, () show that bromelain remains biologically active with a half‐life of 6–9 hr in human body. The enzymatic activity of bromelain covers a wide pH spectrum from 5.5 to 8.0 (Pavan, Jain, Shraddha, & Kumar, ), while the optimum range is 6.0–7.0 at a temperature range of 50–60 ◦ C (Manzoor, Nawaz, Mukhtar, & Haq, ). There are many studies were carried out to investigate the separation, extraction and purification of bromelain from pineapple residues (Coêlho, Silva, Machado, Silveira, & Tambourgi, ; Ketnawa, Chaiwut, & Rawdkuen, ; Martins et al, ; Novaes, Ebinuma, Mazzola, & Júnior, ).…”