Characterization of Bromelain from Parts of Three Different Pineapple Varieties in Nigeria

Abstract

Abstract:Bromelain was extracted from crown, flesh, core, and peel of some variant of Nigerian pineapple fruits in order to evaluate its amount and characteristics. Each part of pineapple after separation was weighed, blended, filtered and then filtrate was precipitated with ethanol and centrifuged. All extracts from each stage were collected and assayed for bromelain activity. The concentration of protein was also measured by using BSA as standard while proteolytic activity was determined using Azocasein 1% (w/v) as substrate at standard conditions. The optimum temperature and pH were also evaluated. The result showed that bromelain could be obtained from all parts of pineapple. Ananas fitzmulleri (Agric) had the highest weight (1486.42 g) and flesh content (53.5%) compared to other varieties while bromelain obtained from all parts and in the different varieties had optimum activity at 40°C and pH of 7. Hence ethanol precipitation method is viable for bromelain recovery.

Addition of Pineapple Hump Extract to Feed and Probiotics in Cultivation Media on the Performance of Catfish (Clarias gariepinus

“…Metode ekstraksi bromelin dari nanas umumnya dilakukan dengan pemberian pelarut buffer fosfat 0,01 M pH 7,0. Kemudian Filtrat yang didapatkan disentrifugasi pada kecepatan 6000g selama 20 menit pada suhu 4ºC (Omotoyinbo, 2017). Metode lain untuk memperoleh aktivitas dan kadar protein tinggi yaitu saat preparasi dilakukan dengan presipitasi menggunakan Amonium sulfat ((NH₄)₂SO₄) dengan konsentrasi 0-60%.…”

Efek Enzim Bromelin dari Bonggol Nanas Terhadap Efesiensi Pakan dan Patologi Anatomi pada Ikan Mas (Cyprinus carpio) yang Diinfestasi Argulus japonicus

“…Enzim dimasukkan kulkas pada suhu 10 o C selama 1 hari hingga terbentuk endapan. Endapan disentrifuge dengan kecepatan 5000 rpm selama 30 menit setelah itu hasil endapan dikeringkan dalam oven pada suhu 40 o C. Endapan yang sudah kering kemudian dihaluskan menggunakan mortal dan alu untuk mendapatkan enzim bromelin kasar berbentuk serbuk (Omotoyinbo and Sanni, 2017). Penggunaan metode tersebut menunjukkan hasil aktifitas enzim bromelin 1,713 U/mL dan hasil konsentrasi enzim bromelin 0,15 mg/ml (Zakiyatussany, 2020).…”