2016
DOI: 10.1590/1519-6984.15915
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Anesthesia by sprinkling method in the gills of tambaqui Colossoma macropomum does not influence intensity and morphology of monogeneans

Abstract: The present study evaluates the influence of anesthesia on the parasitic fauna of monogenea fish parasites, as its intensity and viability. Two experiments were conducted: Evaluation of an anesthetic method by sprinkling eugenol directly on gills and evaluation of monogenea motility and viability; Comparison of immersion and directly sprinkling on the gills with benzocaine and eugenol followed by evaluation on parasite intensity. The results suggest that the anesthetic sprinkling didn't interfere in the parasi… Show more

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Cited by 11 publications
(5 citation statements)
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References 20 publications
(25 reference statements)
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“…No momento da coleta, também foram mensurados os parâmetros de qualidade de água, como: pH (Akrom Kr20®), temperatura e níveis de oxigênio dissolvido (YSI 550A®), condutividade (YSI 30®) e transparência (utilizando-se disco de Secchi). No laboratório, todos os peixes foram previamente anestesiados (eugenol:álcool, 1:10 por aspersão nas brânquias) (Boijink et al, 2017), para realização de biometria, seguida de eutanásia por secção medular para posterior análise parasitológica. Todos os procedimentos com animais foram aprovados pelo Comitê de Ética (nº.…”
Section: Methodsunclassified
“…No momento da coleta, também foram mensurados os parâmetros de qualidade de água, como: pH (Akrom Kr20®), temperatura e níveis de oxigênio dissolvido (YSI 550A®), condutividade (YSI 30®) e transparência (utilizando-se disco de Secchi). No laboratório, todos os peixes foram previamente anestesiados (eugenol:álcool, 1:10 por aspersão nas brânquias) (Boijink et al, 2017), para realização de biometria, seguida de eutanásia por secção medular para posterior análise parasitológica. Todos os procedimentos com animais foram aprovados pelo Comitê de Ética (nº.…”
Section: Methodsunclassified
“…To obtain adult parasites (on average 10 days old at 26°C), fish from laboratory infection were anaesthetized with a solution of eugenol (3 ml (100 l) –1 , a natural anaesthetic recommended for parasitological studies) (Boijink et al, 2016). Live parasites were carefully removed with a needle and scalpel from the surface of the host fish and transferred to a six-well flat-bottom culture vessel (15.5 ml; Becton Dickinson Labware, Franklin Lakes, New Jersey, USA).…”
Section: Methodsmentioning
confidence: 99%
“…Syzigium aromaticum, Myrtaceae), is a recognized anaesthetic in vertebrates and invertebrates (Ross and Ross, 2008) and, according to Boijink et al (2017), is commonly used in Brazilian aquaculture. Its main component, eugenol (4-allyl-2-methoxyphenol), is considered as a safe substance, because of its absence of side effects (Soto and Burhanuddin, 1995;Ross and Ross, 2008).…”
Section: Introductionmentioning
confidence: 99%