Serotypes of Salmonella in Broiler Carcasses Marketed at Ibague, Colombia.

Abstract

Salmonella enterica is a large group of Gram-negative bacteria responsible for a number of foodborne infections associated with the consumption of contaminated poultry products. The hygienic status of raw chicken meat marketed at Ibague, Tolima, Colombia, is currently unknown. To address this issue, a cross-sectional study was conducted to estimate the prevalence of Salmonella spp., in raw chicken marketed at different outlets in this city. Salmonella spp. was isolated by standard microbiological methods, followed by biochemical, serological, and molecular confirmation. Additionally, risk factors associated with the presence of the bacteria were identified. The prevalence of Salmonella in raw chicken was 17.41% (47/270), and 14 different serotypes were found, out of which S. Paratyphi B (36.17%), S. Hvittingfoss (19.15%) and S. Muenster (10.64%) were the most prevalent and represented 65.95% of all serotypes. Amplification of 284 bp of the invA gene was achieved by PCR in a number of randomly selected isolates. Raw chicken as the only type of meat sold at stores (odds ratio: 2,157, p<0.05), and stainless steel as a contact surface of chicken meat (odds ratio: 13,29, p<0.05), were found to be potential risk factors for the presence of Salmonella in chicken meat. This work serves as a reference about the current status of Salmonella in chicken meat marketed in Ibague, Tolima, Colombia, and indicates the need to establish appropriate control and contingency measures to minimize the presence of the bacteria in raw chicken to prevent its transmission to humans.

Prevalence and Molecular Characterization of Salmonella Isolated from Broiler Farms at the Tolima Region—Colombia

“…On the other hand, this variant was the most prevalent in broiler flocks in Belgium (12.3%) and Germany (10.8%) [ 36 ]. However, the result of this study contrasts with the work done by Rodriguez et al [ 26 ], where at least 14 different serovars were isolated from broiler carcasses (47/270) marketed in the capital of Tolima region. As has been discussed in previous works, the results strengthen the hypothesis that contamination of broiler carcasses with a number of potentially pathogenic serovars of Salmonella might be occurring.…”

“…Since the prevalence of Salmonella in broiler carcasses marketed in Ibague city was 17.4% in 2014 and S . Paratyphi B was the serotype most frequently (36.17%) [ 26 ] isolated, together the data suggest the dissemination of S . Paratyphi B from poultry farm to consumer.…”

Multiple antimicrobial resistance of <i>Escherichia coli</i> and <i>Salmonella</i> species isolated from broilers and local chickens retailed along the roadside in Zaria, Nigeria

“…The isolation rate was 10.0% from local chickens and 12.0% from broilers, suggesting there was no difference in the isolation rates from differentially sourced chickens in this study. Our findings were similar to the report of Rodriguez et al (2015) who reported a prevalence of 17.41% of Salmonella spp. in broilers at Ibague, Colombia, and of Al-Khayat & Khammas (2016) who reported a prevalence of 10.4% of Salmonella from layers and broilers in Baghdad.…”

Detection of invA gene of Salmonella from milkfish (Chanos chanos) at Sidoarjo wet fish market, Indonesia, using polymerase chain reaction technique

“…The most common and clinically significant serovar containing the invA virulence gene that causes salmonellosis globally is S . Typhimurium and Salmonella enteritidis [ 19 ].…”