Multiple antimicrobial resistance of <i>Escherichia coli</i> and <i>Salmonella</i> species isolated from broilers and local chickens retailed along the roadside in Zaria, Nigeria

Abstract

have been shown to be resistant to multiple antibiotics. We carried out a bacteriological investigation on 105 cloacal swabs from local and broiler chickens retailed along the roadside in Hanwa, Zaria for the occurrence of E. coli and Salmonella spp. by isolation through culture, and identification using biochemical and serotyping techniques. Serotyping of E. coli isolates was carried out with polyvalent E. coli O157:H7 antisera while Salmonella spp. isolates were serotyped using polyvalent antisera specific for all groups and type-factor for Salmonella spp. Presumptive isolates were subjected to antimicrobial susceptibility testing using 13 panels of antibiotics for both E. coli and Salmonella spp. Results showed that the overall isolation rate of Salmonella spp. was 12 (11.4%), broiler chickens had higher isolation rate 9 (12.0%) of Salmonella than local chickens. However, the isolation rate of E. coli from local chickens 15 (50.0%) was higher compared to broilers 6 (8.0%). Also, non-E. coli O157:H7 and E. coli O157:H7 were isolated from both broilers and local chickens at a frequency of 15 (14.3%) and 6 (5.7%) respectively. The overall isolation rate of E. coli was 21 (20.0%). Multiple antibiotic resistance was observed among local and broiler chickens. However, E. coli and Salmonella species were 100% susceptible to Enrofloxacin. We therefore concluded that E. coli and Salmonella species are prevalent in the cloacae of both broiler and local chickens retailed along the roadside in Hanwa, Zaria, Nigeria and could pose serious veterinary and public health risks.

Isolation and antimicrobial susceptibility pattern of Escherichia Coli from laying chicken and egg in Jimma Town, Ethiopia

“…from egg is in line with the study of Germini et al, 2009. But this finding is lower than some reports from previous works (13). The prevalence of E. coli from cloacae in this study is in line with the privies studies [14,15].…”

Occurrence of Salmonella species in local and commercial chickens slaughtered at Samaru and Sabon-gari live bird markets, Kaduna State

“…Local chickens may acquire drug resistant Salmonella by contact with carriers or by ingestion of food and water that have been contaminated by faecal materials from other scavenging animals, that may have likely received veterinary care and treatment with antimicrobials. Poor sanitation and environmental pollution with human excreta due to inadequate toilet facilities, as observed in many rural communities in Nigeria, may expose local chickens which are free-rangers to resistant bacteria of human origin (Ejeh et al ., 2017). Poor management of effluents generated from abattoirs and commercial farms also contribute to environmental pollution and hence, the possible exposure of free-range chickens to resistant bacteria.…”

Occurrence of Escherichia coli in cloacal samples of broiler chicken from Kollam and Kottayam districts

“…In the present study, isolation and identification of E. coli was done from cloacal swabs of broiler chicken. Similar type of samples were used in various studies conducted by Zinnah et al (2007) from Mymensingh and Ejeh et al (2017) from Nigeria. During the study, the samples were collected in peptone water and further streaked on EMB agar for the selective isolation of E. coli.…”