Detection of invA gene of Salmonella from milkfish (Chanos chanos) at Sidoarjo wet fish market, Indonesia, using polymerase chain reaction technique

Yanestria, Sheila Marty; Rahmaniar, Reina Puspita; Wibisono, Freshinta Jellia; Effendi, Mustofa Helmi

doi:10.14202/vetworld.2019.170-175

Cited by 34 publications

(27 citation statements)

References 17 publications

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“…Data regarding virulence characterization of Salmonella isolates from raw beef origin is rare. However, researchers have well-characterized Salmonella isolates from pork and poultry origin [20,50]. In our research study, we screened Salmonella isolates for seventeen (17) well-known virulence factors.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization Of Pathogenic Salmonella Spp From Raw Beef In Karachi, Pakistan

et al. 2020

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The aim of the present study was to estimate the prevalence of Salmonella and investigate the dominant serovars distribution in raw beef and to screen the isolated serovars for the prescense of beta-lactamases and virulence genes. A total of 150 samples of raw beef sold at butcher shops (n = 75) and supermarkets (n = 75) in Karachi city were collected (50 samples each from muscles, lymph nodes, and minced beef). The samples were cultured according to the ISO-6579-1guidlines. The overall prevalence of Salmonella strains was found to be 21.34%. A total of 56 isolates of Salmonella belonging to four serogroups (Salmonella Pullorum, Salmonella Enteritidis, Salmonella Typhimurium and Salmonella Choleraesuis) were isolated from beef muscles (12%), lymph nodes (24%) and minced beef (28%) samples collected from butcher shops (av. 21.34%). No Salmonella was detected in beef samples collected from supermarkets. S. Enteritidis contamination was highest (37.5%), followed by S. Choleraesuis (30.4%), S. Pullorum (19.6%) and S. Typhimurium (12.5 %). Antibiotic susceptibility testing revealed that Salmonella isolates were highly resistant to Oxytetracycline (90%), Ampicillin (90.5%), Amoxicillin (81.1%), Tetracycline (76%), Neomycin, (79.8%) and Ciprofloxacin (61.4%). The Salmonella isolates examined were more susceptible to the Cephalosporin antibiotics such as Cefixime (43.2%), Cefepime (48.2) and Cefoxitin (49.8%). PCR based screening of blaTEM, blaCTX-M and blaSHV revealed that blaCTX-M and blaTEM were the dominant resistant genes in S. Enteritidis and S. Typhimurium followed by S. Pullorum and S. Choleraesuis whereas blaSHV was the least detected beta-lactamase in Salmonella isolates. Virulence genes screening revealed that at least five genes were present in all the serovars, highest being present in S. Enteritidis (12/17) and S. Typhimurium (12/17). S. Cholerasuis (5/17) carried the least number of virulence genes followed by S. Pullorum (6/17). The present data suggest that beef samples from butcher shops of Karachi city are heavily contaminated with MDR Salmonella. The presence of resistance and virulence genes in MDR strains of Salmonella may play a significant role in transmission and development of Salmonella infection in humans.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization Of Pathogenic Salmonella Spp From Raw Beef In Karachi, Pakistan

et al. 2020

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“…Gram staining was done to differentiate organisms based on the structure of their cell wall [5]. Thin smears from the bacteria colonies were prepared on clean grease free slides; heat fixed slightly and allowed to cool.…”

Section: Gram Staining Techniquementioning

confidence: 99%

“…For instance, cases of fish-borne disease caused by pathogenic microorganisms like Salmonella, Listeria monocytogenes and Vibros spp. has been reported in several parts of the world [4,5].…”

Section: Introductionmentioning

confidence: 96%

“…The use of organic manure also leads to the release of high concentration of opportunistic and pathogenic microorganisms into the ponds which pose a threat not only to fish health but the environment [9]. Also, these microorganisms in fish and fish ponds portend grave consequences for public health [4,5]. Some of these microorganisms possess resistant determinant which enhances their potential for infecting consumers.…”

Section: Introductionmentioning

confidence: 99%

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Bacteriological Assessment and Antibiotics Susceptibility Profile of Bacteria Recovered from Pond Water, Fish Skin, and Gut in Ile-Ife, Osun State, Nigeria

Fakorede¹,

Fatokun²,

Kantiok³

et al. 2019

Preprint

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Fish currently provide 6.7% of all protein consumed by humans globally, nevertheless, aquaculture system has been linked to fish and environmental contamination and disease outbreak. This study aims to isolate, identify, and characterise, bacteria in fish and pond water as well as the antibiotic profile of detected Coliforms. The susceptibility of the isolates was tested using the Kirby-Bauer disc diffusion method on Mueller Hinton agar. A total of forty (40) isolates were isolated from the water samples of which (5) species were Gram Positive bacteria and 35 species of Gram Negative bacteria. The temperature for all ponds ranged from 25°C to 28°C. The mean bacteria count for pond C1 to T2 were 4.9 × 102, 4.9 × 102, 5.4 × 102, 2.5 × 102, 2.2 × 102, and 1.9 × 102 CFU/ml respectively. All isolates were 100% resistant to ceftazidime, cefuroxime and augmentin. More resistance to cefixime (80%) and gentamicin (73.3%) and nitrofurantoin (66.7%) was recorded. However, only 16.6% and 8.3% of the isolates were resistant to ciprofloxacin and ofloxacin respectively. The multiple antimicrobial resistance index (MARI) ranged from 0.5 to 0.9. The water quality parameters (temperature and pH) and the type of bacteria detected in all pond type did not differ significantly. The Multi-drug resistance bacteria detected could be pathogenic to fish and consumers.

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“…For instance, cases of fish-borne disease caused by pathogenic microorganisms like Salmonella, Listeria monocytogenes and Vibros spp. have been reported in several parts of the world [2,3].…”

Section: Introductionmentioning

confidence: 99%

Bacteriological Quality and Antibiotics' Susceptibility Profile of Small-medium Scale Commercial Fish farms in Nigeria

Fakorede¹,

Fatokun²,

Philip-Kantiok³

et al. 2020

TOASJ

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Background: Fish currently provide 6.7% of all proteins consumed by humans globally; nevertheless, the aquaculture system has been linked to fish, environmental contamination and disease outbreak. The aim of this study was to determine the bacteriological quality and the antibiotic resistance profile of bacteria from water samples of pond stocked with Tilapia and Catfish in Ile-Ife, Osun State, Nigeria. Objective: To isolate, identify and characterise heterotrophic bacteria and test for the antibiogram of detected Coliforms. Methods: Water samples were collected from ponds stocked with Tilapia and Catfish, and tested for total heterotrophic and coliform bacteria as well as the antibiogram. The susceptibility of the isolates was tested using the Kirby-Bauer disc diffusion method on Mueller Hinton agar. Results: A total of 40 isolates were recovered from the water samples, of which 5 species were Gram positive bacteria representing two genera, and 35 species were Gram negative bacteria representing four genera. The temperature for all ponds ranged from 25°C to 28°C. The mean bacterial count varied from 1.9×104 to 5.4×104 CFU/ml per fish pond. All isolates were 100% resistant to ceftazidime, cefuroxime and augmentin. More resistance to cefixime (80%) and gentamicin (73.3%) and nitrofurantoin (66.7%) was also recorded. However, only 16.6% and 8.3% of the isolates were resistant to ciprofloxacin and ofloxacin, respectively. The multiple antimicrobial resistance index (MARI) ranged from 0.5 to 0.9. The water physicochemical parameters (temperature and pH) and the type of bacteria detected in all pond types did not differ significantly. Conclusion: Fish pond is a reservoir of multi-drug resistant bacteria that could serve as environmental source of drug resistance gene transfer. This calls for effective monitoring and assessment as well as management devices for the protection of community and environmental health.

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Detection of invA gene of Salmonella from milkfish (Chanos chanos) at Sidoarjo wet fish market, Indonesia, using polymerase chain reaction technique

Cited by 34 publications

References 17 publications

Molecular Characterization Of Pathogenic Salmonella Spp From Raw Beef In Karachi, Pakistan

Molecular Characterization Of Pathogenic Salmonella Spp From Raw Beef In Karachi, Pakistan

Bacteriological Assessment and Antibiotics Susceptibility Profile of Bacteria Recovered from Pond Water, Fish Skin, and Gut in Ile-Ife, Osun State, Nigeria

Bacteriological Quality and Antibiotics' Susceptibility Profile of Small-medium Scale Commercial Fish farms in Nigeria

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