Aim: The study aimed to detect the invA gene in Salmonella isolated from milkfish in the Sidoarjo wet fish market.
Materials and Methods: A total of 84 samples were prepared in enrichment media and isolated on the surface of Salmonella Shigella Agar. Salmonella growth produces transparent colonies with blackish color in the middle due to H2S gas formation. Samples were identified as Salmonella based on macroscopic colony morphology. Presumptive Salmonella sp. was put on Bismuth Sulfite Agar media. Salmonella was determined based on the results of the biochemical test that has been carried out using Microbact identification kits from negative gram staining.
Results: The results of this study indicate that 32 of 84 samples (38.09%) were Salmonella bacteria. Furthermore, the invA gene detection was carried out using the polymerase chain reaction technique. Electrophoresis results showed four positive samples contained invA gene with a length of 284 bp.
Conclusion: Results in this study indicate that contamination of milkfish with Salmonella needs strict hygienic measures to prevent their transmission to human.
Aim: The research was to investigate the antibiotic resistance profile and to screen for methicillin-resistant Staphylococcus aureus (MRSA) from nasal mucosa swab of dogs.
Materials and Methods: The samples were collected from three pet clinics, three K9 units, one veterinary teaching hospital, and one kennel in Surabaya. Of the 50 total samples, 24 confirmed S. aureus strains, which were used for antibiotic sensitivity tests using a disk diffusion method and screening of MRSA used oxacillin resistance screening for base (ORSAB).
Results: This study showed that there were differences in antibiotic resistance patterns among different locations. Fourteen isolates were screened for MRSA by culture on ORSAB.
Conclusion: MRSA carriage was found on nasal swab of dogs, and dogs can act as reservoir of MRSA for spreading to human health.
Keywords: antibiotic-resistant, dogs, methicillin-resistant Staphylococcus aureus, Staphylococcus aureus.
The purpose of this study was to determine the effectiveness of administration of cinnamon (Cinnamomum burmannii) essential oil on hemorrhage, congestion, and inflammatory cell infiltration in the liver of white rats (Rattus norvegicus) induced by streptozotocin to make artificial Diabetes Mellitus (DM). Thirty adult male rats, aged 2-4 months, weighing 130-140 g were divided into six groups. K (-) control group was given drug solvent, K (+) DM rats were given drug solvent, P1 group DM rats were given glibenclamide 0.45 mg / Kg BW + drug solvent, P2, P3, P4 were group of DM rats given oil therapy Cinnamon volatile with doses of 100mg/kg BW, 200mg/kg BW, and 400mg/kg BW, respectively. Therapy was given orally for 14 days. At the end of the study, all experimental animals were euthanized and their livers were taken. The liver was made histopathological preparations with Hematoxylin eosin staining and calculated bleeding, congestion, and inflammatory cell infiltration. The results showed that the lowest number of hemorrhage, congestion, and inflammatory cell infiltration was in the negative control group (K-), the highest number in the positive control group (K+), and the lowest in the treatment group was P3. The results showed a decrease, but no significant difference in hemorrhage. There are significant differences in inflammatory cell congestion and infiltration. The results of this study concluded that cinnamon essential oil can reduce the level of inflammation.
Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak daun salam sebagai pengawet alami pada ikan bandeng di tambak Sidoarjo. Parameter pada penelitian ini adalah organoleptik, pH dan total koloni bakteri pada ikan bandeng. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan 5 perlakuan dengan jumlah ulangan sebanyak 5 kali. Kelompok perlakuan P0 (tanpa perlakuan) sebagai kontrol, P1 (ekstrak daun salam 5%), P2 (ekstrak daun salam 10%), P3 (ekstrak daun salam 15%) dan P4 (ekstrak daun salam 20%). Hasil analisis statistik uji Total Koloni Bakteri serta uji pH menunjukkan perbedaan yang nyata (p<0,05), begitu pula dengan hasil analisis statistik uji organoleptik yang menunjukkan perbedaan nyata tiap perlakuan. Berdasarkan hasil data yang ada, disimpulkan bahwa terdapat pengaruh ekstrak daun salam sebagai pengawet alami pada ikan bandeng terutama pada konsentrasi daun salam sebesar 20%.
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