Prevalence and Molecular Characterization of Salmonella Isolated from Broiler Farms at the Tolima Region—Colombia

Abstract

Salmonella is an important animal and human pathogen responsible for Salmonellosis, and it is frequently associated with the consumption of contaminated poultry products. The aim of this study was to estimate the prevalence of Salmonella in the poultry farms and to determine the genetic relationship. A total of 135 samples collected from fifteen broiler farms, including cloacal, feed, water, environmental and farm operator faeces samples were subjected to microbiological isolation. Molecular confirmation of Salmonella isolates was carried out by amplification of the invA gene, discrimination of d-tartrate-fermenting Salmonella isolates using multiplex PCR, and subsequently analysed by pulsed-field gel electrophoresis (PFGE). A survey questionnaire was conducted to identify potential risk factors for Salmonella presence in broiler farms. The prevalence of Salmonella at the farm level was 26.67%, and Salmonella isolates were serotyped as S. Paratyphi B and all isolates were d-tartrate-fermenting (dT+). PFGE showed three highly similar clusters and one significantly different Salmonella isolate. S. Paratyphi B continued to be present in different links of the poultry chain in the Tolima region, and identification of its main source is necessary to control its dissemination.

Antimicrobial Resistance Profiles and Genetic Typing of Salmonella Serovars from Chicken Embryos in China

“…These results are in agreement with those reported in previous studies [50][51][52]. Moreover, these findings suggest the presence of different sources of contamination, which are in line with previous studies [51,53]. In addition, our results showed that the differences in the PFGE patterns cannot be linked to the difference in antimicrobial-resistant genotypes among the same serovar.…”

Molecular Epidemiology of Salmonella enterica in Poultry in South Africa Using the Farm-to-Fork Approach

“…The results are in agreement with those in previous studies conducted in broiler poultry farms in other countries that have reported a similar prevalence of Salmonella of 35% in Morocco and 31.25% in Bangladesh [ 33 , 34 ]. The results of this study are also higher than those reported in the European Union (EU) (Finland and Sweden: <1%; Ireland: 19.8%), Asia (China: 12.6%; Korea: 15.3%), and South America (Columbia: 17.8%) [ 35 – 38 ]. Studies done in the USA and India along the farm-to-fork pathway reported an overall lower prevalence of 18.1% and 25.8%, respectively, than that in the current study [ 39 , 40 ].…”

“…[33,34]. e results of this study are also higher than those reported in the European Union (EU) (Finland and Sweden: <1%; Ireland: 19.8%), Asia (China: 12.6%; Korea: 15.3%), and South America (Columbia: 17.8%) [35][36][37][38]. Studies done in the USA and India along the farm-to-fork pathway reported an overall lower prevalence of 18.1% and 25.8%, respectively, than that in the current study [39,40].…”

Molecular identification of fluoroquinolone resistance in Salmonella spp. isolated from broiler farms and human samples obtained from two regions in Colombia

“…Our research group has reported the antibiotic resistance patterns of Salmonella spp. in poultry production as well as in other sectors in Colombia, as characterized by different genotyping methods [10][11][12][13][14][15]. However, these studies did not address the molecular mechanisms associated with resistance to quinolones and fluoroquinolones.…”