Inhibition of HMG-CoA reductase activity and cholesterol permeation through Caco-2 cells by caffeoylquinic acids from Vernonia condensata leaves

Arantes, Ana A.; Falé, Pedro L.; Costa, L.C.B.; Pacheco, Rita; Ascensão, Lia; Serralheiro, Maria Luísa

doi:10.1016/j.bjp.2016.05.008

Cited by 30 publications

(17 citation statements)

References 37 publications

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“…Total phenolic compound content was determined spectrophotometrically as described in [32]. The absorbance was measured at 760 nm in triplicates.…”

Section: Total Phenol Content Quantificationmentioning

confidence: 99%

“…The inhibition of the enzymatic activity of HMGR was determined by measuring the decrease in NADPH (Reduced for of Nicotinamide Adenine Dinucleotide Phosphate) absorbance in the presence of HMG-CoA substrate using the HMG-CoA reductase assay kit (Sigma-Aldrich, Barcelona, Spain) following the manufacturer's indications and the process described in [32].…”

Section: Hmg-coa Reductase Activitymentioning

confidence: 99%

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Effect of Food Preparations on In Vitro Bioactivities and Chemical Components of Fucus vesiculosus

André

Guedes

Melo

et al. 2020

Foods

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Fucus vesiculosus is a brown macroalgae used in food and generally considered safe to be consumed, according to EU Directive (EC 258/97). The aim of this study is to analyze the effect of food preparation on F.vesiculosus of different origins on what concerns its chemical constituents and final bioactivities. The aqueous extract of the seaweeds were obtained at different temperatures, similar to food preparation and then purified by SPE. The compound identification was carried out by Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS/MS) and algae extracts microstructure were observed by Scanning Electron Microscopy (SEM). The activities were determined by using antioxidant activity, inhibition of acetylcholinesterase (AChE) and 3-hidroxi-3-methyl-glutaril-CoA (HMG-CoA) reductase (HMGR) together with Caco-2 cells line simulating the intestinal barrier. The activity of AChE and the HMGR were inhibited by the extracts giving IC50 values of 15.0 ± 0.1 µg/mL and 4.2 ± 0.1 µg/mL, respectively and 45% of the cholesterol permeation inhibition. The main compounds identified were phlorotannins and peptides derivatives. The mode of preparation significantly influenced the final bioactivities. Moreover, the in vitro results suggest that the preparation of F. vesiculosus as a soup could have hypercholesterolemia lowering effect.

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“…Total phenolic compound content was determined spectrophotometrically as described in [32]. The absorbance was measured at 760 nm in triplicates.…”

Section: Total Phenol Content Quantificationmentioning

confidence: 99%

Section: Hmg-coa Reductase Activitymentioning

confidence: 99%

Effect of Food Preparations on In Vitro Bioactivities and Chemical Components of Fucus vesiculosus

André

Guedes

Melo

et al. 2020

Foods

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“…[23] reported that grapefruit peels had an IC 50 on HMG-CoA Reductase activity 0.11 μg/ml. Vernonia condensata extract showed the IC 50 value of 271.7 μg/ml [24] and Gnetum gnemon extract had an IC 50 value on HMG-CoA Reductase of 400 μg/ml [25]. There are also studies that have assayed the potency of several isolated chemical contents of the plants in HMG-CoA Reductase inhibition, and it was reported that the compounds inhibit the enzyme activity with the IC 50 value 8.34–149.6 μg/ml [22, 26].…”

Section: Resultsmentioning

confidence: 99%

Influence of extraction methods of bay leaves (Syzygium polyanthum) on antioxidant and HMG-CoA Reductase inhibitory activity

Hartanti

Yonas

Mustamu

et al. 2019

Heliyon

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Objective Bay leaf, one of the plants in Indonesia that has been shown to have activities to reduce cholesterol in the blood. HMG-CoA Reductase inhibition is one of many mechanisms in lowering the level of cholesterol in the blood. Here, we reported the inhibitory activity of HMG-CoA Reductase of bay leaves ethanol extracts that we suspected to be the mechanism of action of bay leaves in reducing cholesterol in the blood. In this research we also investigated the correlation between the inhibitory activities, the total phenol content and antioxidant activities of bay leaves ( Syzygium polianthum ) ethanol extracts. Methods The inhibitory activity of HMG-CoA Reductase was determined kinetically at 340 nm using simvastatin as positive control. In vitro scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric ion reducing antioxidant power (FRAP), and beta-carotene method were used to determine the antioxidant activities. The total phenolic content was determined by Folin-Ciocalteu's method. Results The IC 50 of bay leaves ethanolic extract obtained by percolation and soxhlet extraction method towards HMG-CoA Reductase enzyme activity were 49.50 ± 0.700 μg/mL and 15.50 ± 0.707 μg/mL, respectively, while the IC 50 of simvastatin was 0.00238 ± 0.00004 μg/mL. The antioxidant activity and total phenolic content of bay leaves ethanolic extract obtained by Soxhlet extraction method was higher compared to the percolation method (DPPH and beta-carotene assay results). The 3D linear analysis showed that there was a high correlation between the inhibition activities of HMG-CoA Reductase pattern of both extract types and the total phenol pattern and also the antioxidant pattern of these extracts. Conclusion The result showed that the bay leaves ethanolic extract have a potent activity to reduce the cholesterol serum level by inhibition of HMG-CoA Reductase activity. The activity was due to the phenolic compounds in the extracts as well as the antioxidant activity of the extracts.

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“…effects and have been considered to be beneficial for human health [11]. This compound has antidiabetic, hepatoprotective and antiobesity effects by regulating lipid and glucose metabolism and antioxidant, anti-inflammatory activities [11][12][13][14][15][16][17]. CGA can improve human vascular function, protect vessels against oxidant-induced damage, has direct vasorelaxant effects through endothelium-dependent actions and cause an anti-hypertension activity [11,[18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Oxymel (Sekanjabin-e) Buzuri Syrup as Vascular Opener (Mofatteh) Product and Its Standardization

Yakhchali¹,

Ardakani²,

Vaghasloo³

et al. 2020

JICOA

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Background: Sekanjabin-e buzuri consisting extracts of Chicorium intybus L. (Kasni), Cuscuta chinensis Lam. (Koshus), Apium graveolens L. (Karafs) and Pimpinella anisum L. (Anison) seeds is a Traditional Persian Medicine product. These drugs have many applications in traditional medicine, but they are more effective in opening vascular obstructions and related functions, especially in cardiovascular system. Purpose: In this study we prepared a proper Sekanjabin-e buzuri and developed a HPLC method for analysis of chlorogenic acid (CGA), as an herbal marker compound, for quality control and standardization in both Sekanjabin-e buzuri syrup and its ingredient sources. Methods: Sekanjabin-e buzuri is a group of oxymels that have many different formulations. A proper formulation has chosen from literature (from Gharabadin-e-salehi) and prepared. For standardization of Sekanjabin-e buzuri we developed a method for detecting chlorogenic acid content. A reversed phase MZ C18 column (150*3.0 mm, 5µm) using a mixture of acetonitrile-phosphoric acid 0.1% with gradient elution program for 20 minutes with flow rate of 1.5 ml/min with UV detection at 330 nm. Results: The chlorogenic acid Rt =5.1 minutes and linear over the range of 0.2-1.5 µg/ml, (R2 = 0.9996). The calculated LOD and LOQ of chlorogenic acid were 0.02 and 0.06 µg/ml, respectively. The concentration of chlorogenic acid was 7.69, 10.37, 2.25, 2.88 and 22.86 µg/ml for Chicorium intybus L., Cuscuta chinensis Lam., Apium graveolens L. and Pimpinella anisum L. seeds and Sekanjabin-e buzuri syrup, respectively. Conclusion: This standardized Sekanjabin-e buzuri syrup will be used as a vascular opener (Mofatteh) complementary product for opening internal organs obstruction e.g. promoting cardiovascular health.

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Inhibition of HMG-CoA reductase activity and cholesterol permeation through Caco-2 cells by caffeoylquinic acids from Vernonia condensata leaves

Cited by 30 publications

References 37 publications

Effect of Food Preparations on In Vitro Bioactivities and Chemical Components of Fucus vesiculosus

Effect of Food Preparations on In Vitro Bioactivities and Chemical Components of Fucus vesiculosus

Influence of extraction methods of bay leaves (Syzygium polyanthum) on antioxidant and HMG-CoA Reductase inhibitory activity

Preparation of Oxymel (Sekanjabin-e) Buzuri Syrup as Vascular Opener (Mofatteh) Product and Its Standardization

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