The aim of the research was to study the dynamics of thyroid hormones and thyroid-stimulating hormone of the pituitary gland in Stavropol breed sheep in postnatal ontogenesis and the establishment of thyroid status in animals before insemination, during pregnancy and after childbirth. Determination of concentration of total triiodothyronine, free thyroxine and thyroid stimulating hormone in serum was performed by solid-phase enzyme immunoassay (ELISA) using standard test systems. As a result of the studies, the level of thyroid hormones and thyroid-stimulating hormone in the blood of sheep of Stavropol breed in postnatal ontogenesis was determined. It was established that at the birth of lambs, the lowest level of triiodothyronine (2.55 ± 0.04 nmol/l), thyroxine (19.47 ± 0.45 pmol/l) and thyrotropic (0.31 ± 0.05 Mme/ml) hormone was observed in the blood, compared with other age periods. By the age of three months, the levels of triiodothyronine and thyroxine increased significantly by 7.7% and 24.8%, respectively. When comparing the data in six monthly young ewes, the concentration of all studied hormones was the highest in comparison with other age groups (triiodothyronine – 3.11 ± 0.21 nmol/l; thyroxine – 26.29 ± 0.97 pmol/l; thyrotropic hormone – 0.81 ± 0.09 Mme/ml). In the age of nine months, a significant decrease in triiodothyronine by 16.8%, thyroxine - by 13.2% and thyroid-stimulating hormone - by 34.6% was observed, compared with ewes of 6 month old. By the age of 12 months, only free thyroxine was significantly changed, its serum concentration increased by 9.5% and amounted to 25.21 ± 0.31 pmol/l, compared with the data of nine-month-old sheep. It was found that the concentration of triiodothyronine changes significantly in sheep after childbirth and is 2.53 ± 0.03 nmol/l, which is 6.3% more than in pregnant ewes. The level of thyroxine on the 90th day of pregnancy decreases by 5.3%, and by the 135th day by 16%, compared with the previous period of the study. Thyroid-stimulating hormone in the blood serum of sheep on the 90th day of pregnancy becomes significantly lower by 29% (p ⩽ 0.05) than its concentration in non-pregnant ones. After childbirth, thyroid-stimulating hormone significantly increased by 26.66% (p ⩽ 0.05). As a result of the studies, in sheep of Stavropol breed, standard sizes of the level of thyroid gland hormones (triiodothyronine, thyroxine) and thyroid-stimulating hormone were established.
It is known that the pathological course of pregnancy is associated with a disturbance in the interdependent “mother-fetus” complex and leads to specific clinical consequences at the molecular-cellular, tissue, organ, organismal and population levels of organization. In case of violation of placentation (placental barrier), a state of immune conflict occurs, characterized by an antigen-antibody reaction, carried out through the placenta (in relation to the fetus), or through colostrum after birth (in relation to the newborn offspring). Therefore, immunological relations at the population level should be considered both the consequences of the action of the maternal organism’s antigens on the fetus, and the effects of the allogeneic action of the fetus on the mother’s body. According to the results of the experiment, the placentas of 10 sows of Large White breed were used as the material, which showed signs of isoimmunization to the resulting offspring. For histological examination, immediately after delivery, pieces of placenta up to 0.5 cm thick were selected, which were fixed in a 10% aqueous solution of neutral formalin. The fixed material after wiring through alcohols of increasing concentration, xylene, xylene-paraffin, was poured into paraffin. The obtained preparations were stained with conventional methods-hematoxylin and eosin. d (fibrinoid masses).
The purpose of the study was to research the activity of nucleolar organizers of blood lymphocytes in large white breed pigs in postnatal ontogenesis and during pregnancy. The prepared blood smears were stained with silver nitrate (AgNO3) and additionally stained with azur and eosin. By measuring the parameters of the nucleus and nucleolar organizers’ activity (AgNORs), the changes in the morphological and functional state of the protein synthesizing apparatus of blood lymphocytes in large white breed pigs in postnatal ontogenesis were evaluated. It was found that the area of lymphocyte nuclei in newborn piglets was 83.72±3.37 mm2. By the age of three months, the area of the lymphocyte nuclei of pigs significantly increased by 13.41% (p⩽0.05) and amounted to 96.69±2.57 mm2, at the age of six months this indicator was 15.78% more than at the age of three months (114.80±3.69), by the age of nine months the number of AgNORs significantly (p⩽0.05) decreased by 11.32% compared to six-month-old animals (101.80±2.97). From 1 to 4 units of AgNORs are detected in the lymphocyte nuclei. By the age of three months, the total area of nucleolar organizers’ zones decreased by 29.30% (p⩽0.05), and by the age of twelve months increased by 16.72% (p⩽0.05) compared to the previous age period. A slight increase in the area of the lymphocyte nucleus was observed in pigs after delivery by 9.15% (p⩽0.05). After delivery, the number of AgNORs in pigs significantly increased by 11.26% (p⩽0.05). During pregnancy, from 1 to 3 AgNORs were detected in pigs with a total area of 4.29±0.27 to 4.33±0.30 microns. Based on the conducted research, it can be concluded that the functional state of lymphocytes undergoes obvious changes in postnatal ontogenesis and during pregnancy, this can be seen in alterations in the area of their nuclei, the number and total area of AgNORs.
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