The time course of biotin-vitamer synthesis and the distribution of vitamers were studied in the growing culture of Phycomyces blakesleeanus. Over 92% of the synthesized vitamers accumulated in the culture medium during different stages of incubation period. The remaining part appeared as free and bound forms in the mycelia. The bound form contains only biotin.The vitamers were synthesized after the maximum growth had been attained.The highest growth coincided with the highest degree of assimilation of asparagine.Keto acids were the only detectable acids in the culture medium.P. blakesleeanus accumulated more than 50% dethiobiotin, 26% biotin d-sulphoxide, 15% biotin, and 3% 8-amino-7-ketopelargonic acid with biocytin sulphoxide and biocytin, of the total biotin-vitamers. The ratio of biotin to biotin sulphoxide depends on the culture condition.
The sntihacterial and antifungal effect of fatty acids has been recognized a long tinre ago (SALTON 1968, BYRDE 1969. Generally, fatty acids are t'osic to fungi, and the degree of toxicity depends upon the chain lengt'li, t'he coricentration of t'he acids, and the pH of t'he incubat'ion niediiim (HERTING and DRURP 1974, LIEDEBERG and 1,mr)E-BEKG 1974, SLYNSARENKO et nZ. 1974.I n this coniniiinication we deal with t'he effect of propionic acid on the growt'li arid the ac.ciiirinlation of keto compounds hy different microorganisms, naniely deiobactei uerop,tcx, *Srtcc?iaroiiiyce.s cereoi.siue, P e~i c i l b i u ?~ 'r2otatu,n/, P. chrysopnnni, P. c i t m u y -C P S , and P. oreliciim.Culture media : Aerobacter aeroyenes PRL-R, was grown in shaking rultiires a t 30 "C for 12 hrs on a synt,hetic medium prcvionsly-described by Ilieir 2.4-dinitrophenyl hydrazine derivatives according t o the method of HARPER et al. (1962). Caprat>e and propionate were tit,rinietrically determined after steam distillation according to HORWITZ ct al.(1 970 j.Addition of propionate to A . aeiogenes cultnres affected growth and glucose disappearance to a limited extent (Fig. la). These results are in agreement with reported effects of long chain fatty acids on the other Gram-negative bacteria (GALBRB~TII et ctl. 1971, UALBRAITII and MILLER 1973). However, a n increase in the total keto coinpounds accnitinlation was ohserved (Fig. 1A).A 40% inhibition of growth and 13% increase in the accummilation of total keto conipounds without any change in glucose consulription was reported for the yeast S. ceieaisine in presence of 0.15 propionate (Fig. 1B).Propionate had a strong inhibitory effect on P. notaturn, P. chrysogenunz, and P. citioniyces in surface cultures. The growth inhibition ranged from about 86 to 929; and mas associated with a significant increase in total keto compounds. Under normal conditions and in absence of fatty acids, all Penicilliuni-species tested accuniulate small I ) Supported by a grant (KO. ...
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