Studies on the Biosynthesis of L-Alanine Dehydrogenase by Cuinnghamella elegans

El-Awamry, Zeinab A.; El-Rahmany, T.A.

doi:10.1016/s0232-4393(88)80061-1

Cited by 2 publications

(2 citation statements)

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“…L‫ـ‬ Alanine dehydrogenase ( Lalanine : NAD + oxidoreductase , EC 1.4.1.1 ) is widely distributed in nature and a voluminous literature has appeared in the past fourty years on the enzyme from microorganisms .The enzyme has been extensively studied in several bacteria ( Freese and Oosterwyk ,1963 ;Yoshida and Freese ,1964 ;Germano and Anderson , 1968 ;McCowen and Phibbs , 1974 ;Epstein andGrossowicz , 1976 Elimora et al, 1997 ), actinomycetes ( Roszkowski et al ., 1969 ;Aharonowitz and Friedrich , 1980 ) , algae (Rowell and Stewart , 1976 ) , and fungi ( El -Awamry and El - Rahmany, 1988 ) . Freese and Oosterwyk (1963 ) showed that the formation of L ‫ـ‬ alanine dehydrogenase in B .…”

Section: Introductionmentioning

confidence: 99%

“…erythreus is a substrate inducible enzyme and the induction is not specific ; L-alanine , D ‫ـ‬ alanine and D , L-glutamate being equally good inducer . El -Awamry and El - Rahmany (1988 ) found that L-alanine dehydrogenase of Cunninghamella elegans was produced during logarithmic phase of growth ,and that maximum enzyme synthesis occurred at pH 5-7 . The enzyme was induced by L-alanine, DL ‫ـ‬ alanine, L-aspartic acid, L-asparagine and ammonium phosphate, but L-alanine was the best inducer .…”

Section: Introductionmentioning

confidence: 99%

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STUDIES ON THE BIOSYNTHESIS OF L- ALANINE DEHYDROGENASE BY Thielaviopsis paradoxa

Kadeeb

2002

Journal of Plant Production

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Cell free extracts of Thielaviopsis paradoxa grown on L-alanine as the sole source of nitrogen contained L-alanine dehydrogenase that catalyzes the oxidative deamination of L-alanine to equimolar amounts of pyruvate and ammonia . Lalanine dehydrogenase formation takes place during the logarithmic phase of growth .Maximal growth and enzyme formation were obtained at the 4 th day of growth .The optimum pH for growth and enzyme synthesis was 4.0 . L-alanine dehydrogenase was induced by a great variety of nitrogen sources , but L -alanine , DL-alanine, Ltheronine and ammonium phosphate were the most potent inducers . L-alanine concentration of 2.1 g/ l was found to be optimum for both growth and enzyme synthesis . L-alanine dehydrogenase formation was increased with increasing concentrations of ammonium phosphate in the growth medium ; the optimum concentration was 2.6 g/ l . The effect of different carbon sources and metal salts on growth and enzyme production was studied .

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