The efficiency of yeasts isolated from decaying fruits was investigated. The strains studied were Torulopsis westerdijkii, Paratorulopsis ingeniosa, P. maris, Geotrichum novakii, and four different strains of G. candidum. G. candidum was the most promising new protein producing strain. The effects of molasses, corn steep liquor, and different carbon and nitrogen sources on yeast and protein production by Q. candidurn have been studied. A newly formulated synthetic medium supporting growth and protein yield of G. candidum has been adopted.
Lipids were extracted from the cells of Candida lipolytica grown on pure n-hexadecane and on glucose media. The average free lipids produced from each substrate was 7.2% and 7.8% of cellular dry weight, respectively. Free lipids from the cells grown on each substrate were resolved into 7 fractions by thin-layer chromatography.Quantitative techniques showed that the growth substrate affected the chemical composition of the free lipids. Phospholipids of C. lipolytica grown on n-alkane and on glucose media consisted of, at least, seven different components.These are phosphatidic acid, cardiolipin, phosphatidylglycerol, glycophospholipid (glucose is the sugar base), cephalin, lecithin, and sphingomyelin.
Zusammenfassung
Es wurden Untersuchungen über die Wirkung von Cholinesterase (ChE) in den embryonalen und postembryonalen Stadien von Spodoptera littoralis durchgeführt. Acetylcholin (ACh) wurde in hoher Menge vor allem im Thorax gefunden. Die Larven synthetisieren extrem hohe Beträge von Acetylcholin, unabhängig von der beträchtlichen Menge, die sich in den Rizinus‐blättern an denen die Larven gezogen wurden, befinden. Acetylcholin fand sich im ganzen Körper der erwachsenen Larven, mit Ausnahme des Gehirns. Es wird als ein Wachstumsfaktor betrachtet. In enthirnten Larven, die we‐der Nahrungsaufnahme noch Wachstum zeigten, nahm ACh progressiv ab, während es anscheinend in den physiologisch aktiven Stadien aufgebaut wird. Das cholinergische System wird offenbar nicht in die hormonelle Kon‐trolle der Metamorphose oder Oogenese einbezogen.
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