Investigation on Lipid Contents of Phycomyces Blakesleeanus

Chenouda, M. S.

doi:10.2323/jgam.16.6_501

Cited by 5 publications

(5 citation statements)

References 4 publications

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“…Although the quantity of sterols and SE in the zoospores of B. emersonii seems to exceed the amount in most other Phycomycetes (42), the fatty acid composition of the B. emersonii zoospores does resemble that of other terrestrial phycomycetes (2,7,14,33,39). In addition, 'y-linolenic acid-once thought (33) to be both characteristic of and limited to the phycomycetes, but recently found (32) to occur in other fungi-was associated predominantly with polar lipids in the zoospores we analyzed, as it was in the mixture of variously aged thalli harvested from multiple generation B. emersonii cultures by Sumner (38).…”

Section: Discussionmentioning

confidence: 87%

Lipid Composition of the Zoospores of Blastocladiella emersonii

Mills

Cantino

1974

J Bacteriol

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The zoospores of Blastocladiella emersonii, when derived from cultures grown on solid media, contain about 11% total lipid. This lipid was separated chromatographically on silicic acid into neutral lipid (46.6%), glycolipid (15.8%), and phospholipid (37.6%). Each class was fractionated further on columns of silicic acid, Florisil, or diethylaminoethyl-cellulose, and monitored by thin-layer chromatography. Triglycerides were the major neutral lipids, mono-and diglycosyldiglycerides were the major glycolipids, and phosphatidylcholine and phosphatidylethanolamine were the major phospholipids. Other neutral lipids and phospholipids detected were: hydrocarbons, free fatty acids, free sterols, sterol esters, diglycerides, monoglycerides, lysophosphatidylcholine, lysophos: phatidylethanolamine, phosphatidic acid, phosphatidylserine, and phosphatidylinositol. Palmitic, palmitoleic, stearic, oleic, y-linolenic, and arachidonic acids were the most frequently occurring fatty acids. When B. emersonii was grown in "C-labeled liquid media, lipid again accounted for 11% of both Production of plants and zoospores in liquid media. PYG broth cultures were prepared, inoculated, and induced to release zoospores at 22 C, by the method of Myers and Cantino (26). [1,2-'4C]sodium acetate (New England Nuclear Corp.; 50 pCi) was added 9 h after inoculation, the final concentration being 5 x 10-I M. For studies of zoosporangial lipid, thalli were harvested either just before zoospore cleavage or after zoospores had been cleaved but not released (ca. 23 h after. inoculation); for studies of zoospore lipid, spores were collected about 1 h later.Extraction of lipid. Spore pellets were washed with water, sedimented, sonically treated (30 s, 80 W), and extracted at room temperature with chloro-192 on August 1, 2020 by guest

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Section: Discussionmentioning

confidence: 87%

Lipid Composition of the Zoospores of Blastocladiella emersonii

Mills

Cantino

1974

J Bacteriol

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“…The aquatic forms show the more complex acid spectra and it has been demonstrated that Blastocladiella emeronsii (Sumner, 1970) and certain obligate, marine species (Ellenbogen and Aaronson, 1969) possess both the W3 and the W6 biosynthetic pathways. The zygomycetes can be divided into two groups, with the Mucorales specializing in the synthesis of only two polyunsaturates (Chenouda, 1970) . y-Linolenic acid is ostensibly one of the most significant features of the fatty acids produced by the lower fungi (Shaw, 1966) but it was not possible to determine positional isomers here since none of the isolates under investigation produced a sufficient quantity of the 18: 3 acid for analysis.…”

Section: Materials and Methods-cultures Ofmentioning

confidence: 99%

Identification and Phylogenetic Implications of Fatty Acids and Sterols in Three Genera of Aquatic Phycomycetes

Southall

Motta

Patterson

1977

American J of Botany

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Allomyces macrogynus, A. arbuscula, A. javanicus, Allomyces male and female hybrid strains, Blastocladia ramosa and Monoblepharella sp. were examined for their fatty acid and sterol compositions by GLC and combined GLC/MS. All the organisms produce a range of fatty acids 12 to 20 carbon atoms in length. Palmitic, stearic, and arachidic acid represent the highest concentrations of saturated fatty acids; oleic, linoleic, and arachidonic acid the highest unsaturated fatty acids. B. ramosa synthesizes only two polyunsaturates, linoleic and linolenic, but Allomyces and Monoblepharella are capable of desaturation as far as arachidonic acid. Cholesterol is produced by all the isolates and is the dominant sterol in Allomyces. 24‐Methyl and 24‐ethyl derivatives of cholesterol are the dominant sterols of Monoblepharella. B. ramosa contains a more complex sterol mixture representing changes which occur in the formation of cholesterol from lanosterol: 24‐dihydrolanosterol, 14α‐methyl Δ8‐cholestenol, Δ8(9)‐cholestenol, 14α‐methyl Δ7‐cholestenol, Δ7‐cholestenol and cholesterol. Δ7‐cholestenol, 24‐dihydrolanosterol, and 14α‐methyl Δ8‐choIestenol appear to be the major components. This is the first time that 14α‐methyl Δ8 and 14α‐methyl Δ7‐cholestenol have been reported as naturally occurring sterols.

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“…Interestingly, all the classes of lipids were increased in the light, from three-fold in the case of phospholipids up to twelve-fold for glycerol. Also, compared to carotenoids which are only minor lipid components (less than 1%), these other lipids constitute approximately one quarter of the dry weight in light-grown Red (600-780nm, 11.5 V/nr) 41.5 blakesleeanus (Chenouda, 1970). Apart from the observation that light showed no effect on sterols in minuta (Tada ei.…”

Section: :4 Enzyme Analysis Using a Coupled Systemmentioning

confidence: 99%

“…P. blakesleeanus was found to contain 26-28g free lipids per lOOg dry matter (Chenouda, 1970) (Marouf and Malhotra, 1976). The percentage of unsaturated fatty acids amongst the total lipids was higher in the spores than in the mycelia or stage 1 sporangiophores.…”

Section: Other Lipidsmentioning

confidence: 99%