Lipid Composition of the Zoospores of Blastocladiella emersonii

Abstract

The zoospores of Blastocladiella emersonii, when derived from cultures grown on solid media, contain about 11% total lipid. This lipid was separated chromatographically on silicic acid into neutral lipid (46.6%), glycolipid (15.8%), and phospholipid (37.6%). Each class was fractionated further on columns of silicic acid, Florisil, or diethylaminoethyl-cellulose, and monitored by thin-layer chromatography. Triglycerides were the major neutral lipids, mono-and diglycosyldiglycerides were the major glycolipids, and phosphatidylcholine and phosphatidylethanolamine were the major phospholipids. Other neutral lipids and phospholipids detected were: hydrocarbons, free fatty acids, free sterols, sterol esters, diglycerides, monoglycerides, lysophosphatidylcholine, lysophos: phatidylethanolamine, phosphatidic acid, phosphatidylserine, and phosphatidylinositol. Palmitic, palmitoleic, stearic, oleic, y-linolenic, and arachidonic acids were the most frequently occurring fatty acids. When B. emersonii was grown in "C-labeled liquid media, lipid again accounted for 11% of both Production of plants and zoospores in liquid media. PYG broth cultures were prepared, inoculated, and induced to release zoospores at 22 C, by the method of Myers and Cantino (26). [1,2-'4C]sodium acetate (New England Nuclear Corp.; 50 pCi) was added 9 h after inoculation, the final concentration being 5 x 10-I M. For studies of zoosporangial lipid, thalli were harvested either just before zoospore cleavage or after zoospores had been cleaved but not released (ca. 23 h after. inoculation); for studies of zoospore lipid, spores were collected about 1 h later.Extraction of lipid. Spore pellets were washed with water, sedimented, sonically treated (30 s, 80 W), and extracted at room temperature with chloro-192 on August 1, 2020 by guest

Identification and Phylogenetic Implications of Fatty Acids and Sterols in Three Genera of Aquatic Phycomycetes

“…The average sterol content of fungal organisms ranges from 0.7 to 0.1 % of the dry tissue (Weete, 1974), but the figures obtained for the seven isolates agree with data recorded for phycomycetes and aquatic phycomycetes which range from 0.003 to 0.25 % (McCorkindale et al, 1969;Bean et al, 1972;Mills and Cantino, 1974).…”

Potassium- and calcium-induced alterations in lipid interactions of isolated plasma membranes from Blastocladiella emersonii. Evidence for an adenosine 5'-triphosphate requirement

“…The lipid composition of the plasma membrane preparations was monitored by thin-layer chromatography (TLC) on silica gel G and H using chloroform/methanol/water (65:25:4 v/ v/v) and petroleum ether/diethyl ether/acetic acid (80:20:1 v/v/v) as solvents. Individual lipid components were identified by cochromatography with standards (Supelco), extracted zoospore total, phospho-, glyco-, and neutral lipid samples, and comparisons to previously obtained results (Mills & Cantino, 1974;Leonards & Haug, 1979b, 1980b.…”