Protein production by local yeast strains with special emphasis on <i>Geotrichum candidum</i>

Osman, H. G.; Chenouda, M. S.; El-Masry, H. G.

doi:10.1002/jobm.19730130306

J of Basic Microbiology

1973

DOI: 10.1002/jobm.19730130306

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Protein production by local yeast strains with special emphasis on Geotrichum candidum

H. G. Osman

M. S. Chenouda

H. G. El-Masry

Abstract: The efficiency of yeasts isolated from decaying fruits was investigated. The strains studied were Torulopsis westerdijkii, Paratorulopsis ingeniosa, P. maris, Geotrichum novakii, and four different strains of G. candidum. G. candidum was the most promising new protein producing strain. The effects of molasses, corn steep liquor, and different carbon and nitrogen sources on yeast and protein production by Q. candidurn have been studied. A newly formulated synthetic medium supporting growth and protein yield of … Show more

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1973

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“…Our previous report (OSMAN et al, 1973) showed that Q. candidum, isolated from a local source, is a promising new protein producer. The yeast was grown in the previously formulated synthetic medium (OSMAN et al 1973).…”

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Metabolic activities and dynamic aspects of growth in Geotrichum candidum

Osman

Chenouda

El-Masry

1973

J of Basic Microbiology

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The metabolic activity of Geotrichum candidum was investigated. The chromatographic analysis of the cell pool and of the acid hydrolysate revealed the presence of almost all the amino acids previously detected in other yeasts. The pool pattern was not greatly different from the acid hydrolysate pattern, but i t varied in certain stages of growth.Little information is available concerning the metabolic activity of G. candidum. Our previous report (OSMAN et al, 1973) showed that Q. candidum, isolated from a local source, is a promising new protein producer. The yeast was grown in the previously formulated synthetic medium (OSMAN et al. 1973).The advantage of using a synthetic medium was to avoid possible incorporation of preformed biological compounds into the yeast. Therefore, the occurrence of any metabolite is taken as an ability to synthesize that compound.Material and methods G . candidum isolated from decaying fruits in Egypt was maintained and cultured after OSMAN et al. (1973). Metabolites were determined by the methods adopted by OSMAN and CHENOUDA (1965a). Amino acid pools and acid hydrolysates of the dried cells were qualitatively and quantitatively determined after OSMAN and CHENOUDA (1965b) and MARKH and YURCHENKO (1965) using a DDR extinction recorder. Results and discussion T i m e c o u r s e of p r o t e i n s y n t h e s i s i n r e l a t i o n t o t h e m e t a b o l i c a c t i v i t i e s of G. candidurnThe highest yield of biomass on the 7th day corresponds to the consumption of sucrose and nitrogen in the medium and the synthesis of protein and lipids. The total carbohydrate content of the yeast cells did not change significantly during the fermentation period. The noticeable drop in the p H may be ascribed to the progressive formation of keto acids (Table 1).Chromatographic analysis revealed that a-ketoglutaric and pyruvic acids were the only keto acids detected in the cell pool and the culture medium of G. candidum. The progressive decrease of the keto acid pool may be due to their incorporation in metabolic processes and excretion into the culture medium. I n contrast to the low level of pyruvate in the cell pool, this compound was found in large amounts in the culture medium. This may be either due t o the high permeability of yeast cells to pyruvate or t o its transient metabolic production. The presence of the keto acids may indicate the functioning of the tricarboxylic acid cycle in G. candidum.

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confidence: 99%

“…candidum isolated from decaying fruits in Egypt was maintained and cultured after OSMAN et al (1973). Metabolites were determined by the methods adopted by OSMAN and CHENOUDA (1965a).…”

mentioning

confidence: 99%

Metabolic activities and dynamic aspects of growth in Geotrichum candidum

Osman

Chenouda

El-Masry

1973

J of Basic Microbiology

Self Cite

View full text Add to dashboard Cite

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Protein production by local yeast strains with special emphasis on Geotrichum candidum

Cited by 1 publication

References 7 publications

Metabolic activities and dynamic aspects of growth in Geotrichum candidum

Metabolic activities and dynamic aspects of growth in Geotrichum candidum

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