The fluorescence spectra have been measured from endogenous (native) fluorophores in normal, diseased and tumor thyroid tissues. They were found to be sufficiently different for the technique to be used as the basis for tumor diagnosis. Papillar carcinoma. Fluorescence spectra emitted from normal (dashed line) and cancerous (solid line) thyroid tissues

We have applied the Laser‐Induced Fluorescence technique to thyroid tissues. A close correlation between spectral shapes and the degree of tissue abnormality have been found. In some cases fluorescence spectrum is formed by linear superposition of “healthy” and “pathological” basic spectral shapes. This allows to ca determine unambiguously the state of the tissue by means of any single spectral measurement. (© 2006 by Astro, Ltd. Published exclusively by WILEY‐VCH Verlag GmbH & Co. KGaA)

Laser induced fluorescence (LIF) with wide area surveillance for resected thyroid tissue solid chunks is presented. The characteristic LIF spectra of goiter were established. The state of tissue at each point represents a superposition of normal and pathology states. To our knowledge two co-existing pathological effects were observed optically for the first time. It is demonstrated that the LIF spectral functions and their intensities well-labeled such areas and represent a good tool for medical diagnostics of goiter and for the definition of the degree of abnormality and geometrical sizes of these areas. LIF spectra from different points of a thyroid tissue solid chunk

We applied the laser induced fluorescence spectroscopy (LIFS) to investigate intestinal and liver tissues of normal male Wistar rats fed with vitamin A. The special procedure based on intensity spectral functions fitting was developed for the recognition of vitamin A in different tissues. Based on this procedure it is demonstrated that the LIFS can be used to monitor vitamin A deposition and distribution in the body of rat, which is essential for understanding the mechanism of formation of the vitamin A rich droplets, as the mechanism of vitamin A mobilization.