Study of vitamin A distribution in rats by laser induced fluorescence spectroscopy

Akhmeteli, K T; Ekaladze, Eka; Jaliashvli, Z V; Medoidze, T D; Melikishvili, Zaza G.; Merkviladze, N; Papava, M; Tushurashvili, P

doi:10.1002/lapl.200810012

Cited by 5 publications

(7 citation statements)

References 13 publications

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“…HSCs are reactive to the Golgi staining, which is usually used for neurofilament staining (Wake, 1980). Recently, the laser‐induced fluorescence spectroscopy has been applied to investigate intestinal and liver tissue of normal and vitamin A‐fed rats (Akhmeteli et al, 2008). A special procedure based on intensity spectral functions fitting was developed for the recognition of vitamin A in different tissues.…”

Section: Structure Of Hscsmentioning

confidence: 99%

Hepatic stellate cell (vitamin A‐storing cell) and its relative – past, present and future

Senoo

Yoshikawa

Morii

et al. 2010

Cell Biology International

175

133

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HSCs (hepatic stellate cells) (also called vitamin A-storing cells, lipocytes, interstitial cells, fat-storing cells or Ito cells) exist in the space between parenchymal cells and liver sinusoidal endothelial cells of the hepatic lobule and store 50-80% of vitamin A in the whole body as retinyl palmitate in lipid droplets in the cytoplasm. In physiological conditions, these cells play pivotal roles in the regulation of vitamin A homoeostasis. In pathological conditions, such as hepatic fibrosis or liver cirrhosis, HSCs lose vitamin A and synthesize a large amount of extracellular matrix components including collagen, proteoglycan, glycosaminoglycan and adhesive glycoproteins. Morphology of these cells also changes from the star-shaped SCs (stellate cells) to that of fibroblasts or myofibroblasts. The hepatic SCs are now considered to be targets of therapy of hepatic fibrosis or liver cirrhosis. HSCs are activated by adhering to the parenchymal cells and lose stored vitamin A during hepatic regeneration. Vitamin A-storing cells exist in extrahepatic organs such as the pancreas, lungs, kidneys and intestines. Vitamin A-storing cells in the liver and extrahepatic organs form a cellular system. The research of the vitamin A-storing cells has developed and expanded vigorously. The past, present and future of the research of the vitamin A-storing cells (SCs) will be summarized and discussed in this review.

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Section: Structure Of Hscsmentioning

confidence: 99%

Hepatic stellate cell (vitamin A‐storing cell) and its relative – past, present and future

Senoo

Yoshikawa

Morii

et al. 2010

Cell Biology International

175

133

View full text Add to dashboard Cite

show abstract

“…The measured absorption efficiency (13-36%) was compared to the absorption efficiency of 58% computed using the precise theoretical method for a gaussian beam of Refs. [1][2][3] (see the details at the end of this section). Thus, the experimental results are in qualitative agreement with the theoretical predictions derived for gaussian beams.…”

Section: Measurement and Computation Methodsmentioning

confidence: 99%

“…A theoretical possibility of non-resonant, fast, and efficient heating of very thin conducting cylindrical targets by coaxial broad electromagnetic beams was described in Ref. [1] (see also the sections on the "longitudinal geometry" in Refs. [2,3] and references there).…”

Section: Introductionmentioning

confidence: 99%

“…This possibility can be used for such applications as pumping of active media of short-wavelength lasers and creation of low-density channels in the lower atmosphere (Ref. [1]). Recently, experimental confirmation of efficient heating of extremely thin conducting cylindrical targets by broad electromagnetic beams was obtained in Ref.…”

Section: Introductionmentioning

confidence: 99%

“…In this work we present a qualitative experimental confirmation of the predictions of Refs. [1][2][3]…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Efficient non-resonant absorption of electromagnetic radiation in thin cylindrical targets: experimental evidence

Akhmeteli¹,

Kokodiy

Safronov

et al. 2014

SPIE Proceedings

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A theoretical possibility of non-resonant, fast, and efficient (up to 40 percent) heating of very thin conducting cylindrical targets by broad electromagnetic beams was predicted in [Akhmeteli, arXiv:physics/0405091 and 0611169] based on rigorous solution of the diffraction problem. The diameter of the cylinder can be orders of magnitude smaller than the wavelength (for the transverse geometry) or the beam waist (for the longitudinal geometry) of the electromagnetic radiation. Experimental confirmation of the above results is presented [Akhmeteli, Kokodiy, Safronov, Balkashin, Priz, Tarasevitch, arXiv:1109.1626 and 1208.

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Changes in laser-induced fluorescence responses of 3T3 fibroblasts to repetitive thermal stress

et al. 2009

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Abstract:The combined experimental use of laser-induced autofluorescence of cellular metabolites and methodological fundamentals of systems biology will provide access to biological thermal stress analysis on a sub cellular level. A test setup incorporating a pulsed nitrogen laser was realized with which autofluorescence of the coenzyme NADH could be measured in living 3T3 cells. The cells were subjected to different temperature stress at repetitive time intervals. When subjected to a simple mathematical analysis, the NADH concentration change measured through autofluorescence in biological cells exhibited approximate concentration-equivalent balance curves. These results add up to the fundamental know-how about the dosimetry of thermally therapeutic methods.

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Study of vitamin A distribution in rats by laser induced fluorescence spectroscopy

Cited by 5 publications

References 13 publications

Hepatic stellate cell (vitamin A‐storing cell) and its relative – past, present and future

Hepatic stellate cell (vitamin A‐storing cell) and its relative – past, present and future

Efficient non-resonant absorption of electromagnetic radiation in thin cylindrical targets: experimental evidence

Changes in laser-induced fluorescence responses of 3T3 fibroblasts to repetitive thermal stress

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