Changes in laser-induced fluorescence responses of 3T3 fibroblasts to repetitive thermal stress

Beuthan, J.; Dressler, Cathrin; Zabaryło, Urszula; Minet, Olaf

doi:10.1002/lapl.200810134

Cited by 8 publications

(12 citation statements)

References 20 publications

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“…A significant amount of H + protons is released during the enzyme activity of the NADH dehydrogenase. H + protons are pumped into the mitochondrial intermembrane space by both the NADH hydrogenase and the cytochrome C complex, together with the cytochrome oxidase [49,50]. In this part when the spectrum of near 785 nm wavelength is absorbed by the ferrous heme a +2 3 in the c-oxidase cytochrome of the mitochondria [14], promotes rapid modifications in the cascade of intracellular chemical and physical reactions, leading to metabolic alterations [51].…”

Section: Discussionmentioning

confidence: 99%

“…After exposure to the experimental conditions, the culture medium was removed and the viable cells that remained adhered to the glass substrate were fixed in 1 mL of buffered 2.5% glutaraldehyde for 24 hours and post-fixed with 1% osmium tetroxide for 1 hour. The cells adhered to the glass substrate were then dehydrated in a series of increasing ethanol concentrations (30,50,70,95, and 100%) and immersed in 1,1,1,3,3,3-hexamethyldisilazane (HMDS; Acros Organics, Springfield, NJ, USA) for 90 minutes and stored in a desiccator for 24 hours. The cover glasses were then mounted on metallic stubs, sputter-coated with gold and the morphology of the surface-adhered and MDPC-23 cells was examined with a scanning electron microscope (JEOL-JMS-T33A Scanning Microscope, Tokyo, Japan).…”

Section: Analysis Of Cell Morphology By Scanning Electron Microscopy mentioning

confidence: 99%

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In vitro effect of low-level laser on odontoblast-like cells

et al. 2010

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The aim of this study was to evaluate the metabolism of odontoblast-like MDPC-23 cells subjected to direct LLL irradiation. The cells were seeded (20,000 cells/well) in 24-well plates and incubated for 24 hours at 37• C. After this period, the culture medium (DMEM) was replaced by fresh DMEM supplemented with 2 or 5% (stress induction by nutritional deficit) or 10% fetal bovine serum (FBS). The cells were exposed to laser doses of 2, 4, 10, 15 and 25 J/cm 2 from a near infrared InGaAsP diode laser prototype (LASERTable; 780±3 nm, 40 mW). One control group (sham irradiation) was established for each experimental condition (laser dose x FBS supplementation). Three and 72 hours after the last irradiation, cells were analyzed with respect to metabolism, morphology, total protein expression and alkaline phosphatase (ALP) activity. Higher metabolism and total protein expression were observed 72 hours after the last irradiation at the doses of 15 and 25 J/cm 2 (Mann-Whitney; p<0.05). Higher ALP activity was obtained with 5% FBS when the cells were irradiated with doses of 2 and 10 J/cm 2 . For the dose of 25 J/cm 2 , the highest ALP activity was observed with 10% FBS. It was concluded that the LLLT parameters used in this study stimulated the metabolic activity of the MDPC-23 cells, especially at the doses of 15 and 25 J/cm 2 .

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Section: Discussionmentioning

confidence: 99%

Section: Analysis Of Cell Morphology By Scanning Electron Microscopy mentioning

confidence: 99%

In vitro effect of low-level laser on odontoblast-like cells

et al. 2010

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“…This fact cannot be described in a systemic context and needs to be analyzed as specific issues such as NAD(P)H coenzyme autofluorescence [28,39,40,44], intracellular calcium metabolism [15,16,41,42], and various subcellular structures [19] using analytical and numerical modeling [38,40,[43][44][45].…”

Section: Resultsmentioning

confidence: 99%

Thermally induced changes of optical and vital parameters in human cancer cells

et al. 2010

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Minimally invasive laser-induced thermotherapy (LITT) presents an alternative method to conventional tumor therapeutically interventions, such as surgery, chemotherapy, radiotherapy or nuclear medicine. Optical tissue characteristics of tumor cells and their heat-induced changes are essential issues for controlling LITT progressions. Therefore, it is indispensable to exactly know the absorption coefficient μ a , the scattering coefficient μ s and the anisotropy factor g as well as their changes under rising temperatures in order to simulate the treatment parameters successfully. Optical parameters of two different cancer model tissues -breast cancer cells species MX1 and colon cancer cells species CX1 -were measured in the spectral range 400 -1100 nm as well as in the temperature range 37 -60• C. The absorption coefficient of both cell species was low throughout the spectral range analyzed, while μ s of both species rose with increasing temperatures. The anisotropy factor g however dropped for both tissues with increasing temperatures. Light scatterings inside tissues proceeded continuously forward for all species tested. It was demonstrated that optical tissue properties undergo significant changes along with the vital status of the cells when the temperature increases. Screening of the metabolic activities of MX1 and CX1 cells after exposure to heat stress

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“…1), the fiber sensor was moved inside the sample throughout the experiment. However, since it was For an approximate mathematical evaluation, the function 1 from previous experiments [17] was used again:…”

Section: Resultsmentioning

confidence: 99%

Characterization of the respiration of 3T3 cells by laser-induced fluorescence during a cyclic heating process

Beuthan¹,

Dressler²,

Zabaryło³

et al. 2010

Laser Phys. Lett.

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The use of lasers in the near infrared spectral range for laser-induced tumor therapy (LITT) demands a new understanding of the thermal responses to repetitive heat stress. The analysis of laser-induced fluorescence during vital monitoring offers an excellent opportunity to solve many of the related issues in this field. The laser-induced fluorescence of the cellular coenzyme NADH was investigated for its time and intensity behavior under heat stress conditions. Heat was applied to vital 3T3 cells (from 22• C to 50• C) according to a typical therapeutical time regime. A sharp increase in temperature resulted in non-linear time behavior when the concentration of this vital coenzyme changed. There are indications that biological systems have a delayed reaction on a cellular level. These results are therefore important for further dosimetric investigations.

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Changes in laser-induced fluorescence responses of 3T3 fibroblasts to repetitive thermal stress

Cited by 8 publications

References 20 publications

In vitro effect of low-level laser on odontoblast-like cells

In vitro effect of low-level laser on odontoblast-like cells

Thermally induced changes of optical and vital parameters in human cancer cells

Characterization of the respiration of 3T3 cells by laser-induced fluorescence during a cyclic heating process

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