The purposes of this study were to investigate the effect of administration of GnRH and PGF2α on onset of estrus and conception rate in different postpartum swamp buffalo (Bubalus bubalis). Twenty postpartum (pp) buffalo-cows were divided into four groups of five buffaloes as follows: Group A (30 day pp), Group B (45 dpp), Group C (60 dpp), and Group D (75 dpp). The buffaloes in each group were injected intramuscularly with 3 ml GnRH at day 0, followed by intramuscular injection of 2.5 ml PGF2α, 10 ml) at day 7 after GnRH injection. Observation of estrus was performed 24 h after injection of PGF 2α. Artificial insemination was performed 18 h after estrus signs clearly visible. The result of study showed that there were no difference (p>0.05) among treatments on the percentages of estrus (100%), onset of estrus (37.4 to 38.4 h), duration of estrus (16.8 to 18.2 h) and conception rate (100%). In conclusion, buffalo-cows in Kampar Regency can be served as early as 30 days post partum using combination of 3 ml GnRH and 2.5 ml of PGF2α.
The purpose of this study was to know the quality of Boer liquid semen during storage by adding sweet orange essential oil and gentamicin into the tris yolkextender.The semenlongevity test was carried out by storing semen in a closed tube at room temperature and refrigerator, and evaluated motility and Viability every 3 hours at room temperature and 12 hours in refrigerator. The results showed that the characteristics of the liquid semen, with the addition of 1% of essential oils showed percentage of motility and Viability significantly higher than 0.5% and without the addition of essential oil, either stored at room temperature or in the refrigerator. This may be related to the content of essential oils of sweet orange peel containing flavonoids and antibacterials that are capable of maintaining liquid semen quality of Boer Goat.In addition, gentamicin contains antibacterials capable of suppressing the growth of bacteria which can damage and durabilitythe spermatozoa.Keywords: Boer Goat,Gentamicin, Liquid semen, Sweet orange
The purpose of this study was to determine the effect offertilization time and dffirent incubation systems onfertilization level by in-vitro. The mature of ovaries from indigenous cow andfresh cementfrom HolsteinFrisiancows(FH),0.9% NaClphysiological PBS,NissuiJapan, I,HEPES30pM,20 mL Heparin, 10% goat serum, 250 g/ml FSH BO medium, medium gentamisain 50 mB-O, mineral oil, alcohol, aquabidest, and I %o aceto orcein were materials and reagents. The Completely Randomized Design (CRD) in factorial pattern was used. Results shown no significant ffict on the percentage of fertitized ooq)tes and level of development of pronuclei (2PN and> 2PN) by different timing offertilization and incubation system. The development of pronucleus (IPN) showed significant (P <0.05) on I2 hours (37.60 %o), but no significant effect on dffirent incubation system. It concluded, the system of incubation and time of fertilization has no effect on oocyte fertilization rate. Oocytes fertilization time can be performed at 6 hours , I 2 hours, and I B hours, while the extension of the period of fertilization until I 8 hours did not increase the I ev el offertilization.
This paper aims to find out the application of I.B. in cows and the control of estrus to determine fertility and conception rate in cows. The research was conducted in two stages: field research to determine the influence of I.B. time on conception rate. Local cows in I.B. amounted to 62 heads, and crossbred cows with as many as 100 heads were grouped based on I.B. time and parity and analysed with the Z test. Fertility is determined by the size of the dominant follicle using ultrasonography, and the number of gardenings is determined 30 days after I.B. and 60 days of rectal palpation. Average conception rate at the beginning of estrus (45.70%), middle estrus (60.59%), end of estrus (68.91%), and six hours after estrus (61.55%). I.B. time had a significant effect (P<0.05) on the conception rate. Based on the results of this study, it was revealed that parity-3 had the highest pregnancy rate for local cattle (70.34%), while crossbreed cattle had the highest pregnancy rate at parity-2 (56.79%). The follicle size in Bali cattle was the same as that of local south coast cattle, including the small follicle size category.
This research aim to know the influence of depth veneering time of spermatozoa at media of TALP which was supplemented by 4% BSA to embryo gender in in-vitro and to get the time dissociation of spermatozoa which have the best chromosome Y. This research represent attempt of exsperiment in RBT by 4 time veneering of spermatozoa as treatment, times intake of semen of epididymis to groups. Variables measure are: 1). mortality of spermatozoa, 2). number of in vitro fertilzation, 3. masculine gender embryo ratio. Result shown, the veneering of cow spermatozoa during 90 minute in media of TALP which was supplemented by 4% bovine serum albumin (BSA) can dissociate spermatozoa which have chromosome Y with percentage of highest male sex (90.00±24.49), and highest percentage of mortality and fertility were obtained at veneering during 60 minute with scoring 73.33 ± 5.16 and 53.92±18.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.