Pengaruh Waktu Fertilisasi dan Sistem Inkubasi yang Berbeda terhadap Tingkat Fertilisasi Sapi Lokal Secara In Vitro

Abstract

The purpose of this study was to determine the effect offertilization time and dffirent incubation systems onfertilization level by in-vitro. The mature of ovaries from indigenous cow andfresh cementfrom HolsteinFrisiancows(FH),0.9% NaClphysiological PBS,NissuiJapan, I,HEPES30pM,20 mL Heparin, 10% goat serum, 250 g/ml FSH BO medium, medium gentamisain 50 mB-O, mineral oil, alcohol, aquabidest, and I %o aceto orcein were materials and reagents. The Completely Randomized Design (CRD) in factorial pattern was used. Results shown no significant ffict on the percentage of fertitized ooq)tes and level of development of pronuclei (2PN and> 2PN) by different timing offertilization and incubation system. The development of pronucleus (IPN) showed significant (P <0.05) on I2 hours (37.60 %o), but no significant effect on dffirent incubation system. It concluded, the system of incubation and time of fertilization has no effect on oocyte fertilization rate. Oocytes fertilization time can be performed at 6 hours , I 2 hours, and I B hours, while the extension of the period of fertilization until I 8 hours did not increase the I ev el offertilization.

Pengaruh Keberadaan Corpus Luteum Terhadap Kualitas Oosit dan Tingkat Maturasi Oosit Kerbau Secara In Vitro

“…Keunggulan teknik PEIV salah satunya adalah dalam upaya penyelamatan materi genetik dari ternak yang mengalami gangguan pada fungsi reproduksi, ternak yang mengalami kematian atau mengalami penyakit dan gangguan pada fungsi tubuh. Teknik ini memungkinkan pelaksanaan proses maturasi oosit, fertilisasi oosit dengan spermatozoa serta perkembangan embrio dapat dilakukan pada lingkungan buatan di luar tubuh dalam suatu sistem biakan sel (Syaiful et al, 2011). Karena oosit adalah tujuan utama dari pemanfaatan ovarium dari RPH oleh karena itu kualitas serta kompetensi oosit perlu untuk diperhatikan karena hal ini merupakan parameter penting dalam proses maturasi in vitro serta akan menentukan tingkat keberhasilan perkembangan embrio.…”

Effect of Adding Insulin Transferrin Selenium (ITS) in the Medium on Maturation and Fertilization Rates of Bali Cattle Oocytes

“…𝑇ℎ𝑒 𝑛𝑢𝑚𝑏𝑒𝑟 𝑜𝑓𝑜𝑜𝑐𝑦𝑡𝑒𝑠 𝑢𝑛𝑑𝑒𝑟 𝑔𝑜𝑖𝑛𝑔 𝑎𝑠 𝑡𝑎𝑔𝑒 𝑜𝑓 𝑚𝑎𝑡𝑢𝑟𝑎𝑡𝑖𝑜𝑛 𝑇ℎ𝑒 𝑛𝑢𝑚𝑏𝑒𝑟 𝑜𝑓 𝑜𝑜𝑐𝑦𝑡𝑒𝑠 𝑏𝑒𝑖𝑛𝑔 𝑠𝑎𝑡𝑢𝑟𝑎𝑡𝑒𝑑 X100% Meanwhile, fertilization rates according to Syaiful et al (2011) was fragmented oocytes or oocytes that do not achieve metaphase II development (0 PN); oocytes that have one pronucleus (1 PN) consisting only of female pronucleus; oocytes that have two pronucleus (2 PN) consisting of male and female pronucleus; fertilized oocytes that have two or more pronucleus (> 2 PN).In vitro fertilization rates may be calculated according to the formula below:…”

Tingkat Kematangan Inti Oosit Sapi Setelah 24 Jam Presevasi Ovarium

“…Corresponding author : widyastuti25@gmail.com DOI: http://dx.doi.org/10.17969/agripet.v15i2.2417 (Totey et al,1993), domba (Syaiful et al, 2011), kambing (Pawshe et al, 1994 kucing, anjing dan cheetah (Beveridge dan Jabtour, 1998). Produksi embrio melalui IVF dapat menggunakan oosit yang berasal dari Rumah Potong Hewan (RPH) maupun dari hewan hidup yang diperoleh melalui teknik Ovum Pick-Up (OPU) dengan bantuan ultrasonografi (Ptak et al, 1999dan Kochhar et al, 2002.…”