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The patient was a 5-day-old boy born at 37 weeks and 4 days. At birth, an amniotic band encircling and constricting his left middle finger was noted, in addition to multiple anomalies including a right-side cleft lip and palate, a club foot, and syndactyly on the left hand. We performed cheiloplasty at 5 months, and palatoplasty at 18 months.

Prototype strain, M-25 , of parainfluenza type 4 (PI-4) virus was grown to high titers and passaged serially in Vero (Cercopithecus kidney cell line) cell cultures under fluid overlay containing low concentrations of trypsin. Infectivity titers in both primary cercopithecus kidney cell cultures (VK) and in Vero cell cultures under ordinary conditions (Vro) did not change essentially through passages in the trypsin-added Vero cell cultures (Vrt) from those of the original VK-grown virus. The 14th Vrt-passage virus was not transferable in Vro beyond two subpassages. Consequently, occurrence of adaptation to Vro during passages in Vrt can be excluded. Identification of V rt-grown virus as PI-4 was demonstrated by the cross neutralization and hemagglutination-inhibition tests performed among paramyxoviruses and b y several biological a nd physico-chemical tests. Other proteolytic enzymes , pancreatin and pronase, gave similar results as did trypsin, but hyaluronidase and lysozyme were ineffective. It was found that animal sera were inhibitory to the trypsin-effect mentioned above. Therefore, the Vrt system must be serum-free for obtaining reproducible results .

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Objectives To determine the late complications and remaining patients). Two of 32 patients with an intussuscepted and invaginated ileal nipple required consequences for renal function, vitamin and acidbase metabolism after application of the Mainz Pouch re-operation due to incontinence, but none of the patients with an appendiceal stoma were incontinent. I (MZP-I) technique in children and young adults. Patients and methods To November 1994, the MZP-I Open revision of a stomal stenosis was performed in three and endoscopic treatment in nine patients. In procedure was carried out in 463 patients at our institution, 91 of whom were children and adolescents 54 patients, the levels of vitamins A, B 1 , B 2 , B 6 , E, folic and bile acid were within normal ranges. There (∏20 years old) using bladder augmentation in 21 and a continent cutaneous stoma in 70. A minimum was no significant decrease in vitamin B 12 levels after operation. In none of the patients with normal follow-up of 1 year was possible in 87 patients or 163 renal units (RUs) with a mean of 5.5 years (range pre-operative creatinine values had the levels increased and none developed severe acidosis or bowel 1-10.5).Results At the last examination, 23 of 55 (42%) preneoplasm. Conclusion The MZP-I is recommended as a technique operatively dilated RUs had improved, 131 of the 163 RUs (80%) were stable and nine RUs (5.5%) showed for bladder augmentation or continent urinary diversion in children and young adults, with an acceptable a slight clinical asymptomatic increase in the upper tract dilatation. Through an extraperitoneal flank complication rate which offers long-term protection of the upper urinary tract. incision, 11% of the RUs which developed stenosis at the ureterocolic anastomosis were successfully reimKeywords Continent urinary diversion, children, upper urinary tract, complications, metabolic planted (16% in patients with neurogenic disorders, 17% with pre-operative irradiation and 5% in the The MZP-I procedure was introduced in 1983 [2] and

To assess the differential stimulus to central and intravascular osmoreceptors during recovery from thermal dehydration, we measured Na concentrations in cerebrospinal fluid ([Na]CSF) and plasma ([Na]p) continuously and compared these during simulated drinking by gastric water infusion (INF) in euhydrated and thermally dehydrated rats under anesthesia. Continuous measurement of [Na]CSF was obtained with a double-barreled Na electrode placed in the lateral ventricle. Continuous measurement of [Na]p was obtained from a flow cell Na electrode in an extracorporeal shunt. Measurements were made during 10 min of INF (2.5 ml/100 g body wt) into the stomach and during 20 min of recovery. Changes in [Na]CSF always lagged behind those in [Na]p and were quantitatively smaller after INF. The decrease in [Na]CSF occurred sooner in dehydrated than in euhydrated rats in response to the decrease in [Na]p (P < 0.01). These results suggest that water and/or Na movement between blood and CSF is accelerated during restitution from thermal dehydration, acting to prevent overhydration during the early phase of rehydration.

We have studied methods for testing the neurovirulence of live poliovaccine viruses by intraspinal inoculation into mice carrying the human poliovirus receptor gene (Tg mice). A comparison of the neurovirulence of Sabin type 3 vaccine virus and related viruses using the 50% paralysis dose determined after intraspinal inoculation into the Tg mice as an index revealed a close correlation between the results of the paralysis dose in Tg mice, the neurovirulence expressed by the histopathological lesions core in monkeys, and the temperature sensitivity of the viruses. The results of experiments in the Tg mice also showed a good correlation with the number of mutations at position 472 from U to C in the 5' noncoding region in the genomes of the viruses tested. These results strongly suggest that the neurovirulence test for oral poliomyelitis vaccine using the Tg mice is an excellent method and may be used in place of the test using monkeys.