Properties of a Cytopathic Agent Isolated From a Patient With Subacute Sclerosing Panencephalitis in Japan

Mode of Subacute Sclerosing Panencephalitis (SSPE) Virus Infection in Tissue Culture Cells

“…Vero/SSPE-N1 has been thought to be defective in production of infectious virions (5). The present study demonstrated the presence of cell-free infectious agents by means of freezing and thawing and hypotonic treatment of the cells.…”

“…These SSPE agents are different from one another in some respects, especially in ability to produce virions. Some produce infectious virions (7, 16) but others do not (2,5,6,11,15,17). In general, the budding structure in cells infected with SSPE agents is hardly detectable when compared to measles virus infection (12,14,16).…”

“…Niigata-1 strain was isolated in Japan by cocultivation of a brain biopsy specimen from a SSPE patient with Vero or HeLa cells (5 Measles virus.Edmonston strain of measles virus was grown in Vero cells. Culture fluids of infected cells were clarified by a centrifugation at 2,000 x g for 10 min to remove cell debris and the supernatant fluid was used as the virus source.…”

Antibody Response to Structural Proteins of Measles Virus in Patients With Natural Measles and Subacute Sclerosing Panencephalitis

“…SSPE virus, such as cell-associated Niigata-1 (Doi et al, 1972), ZH and SI (Mirchamsy et al, 1978) strains were grown by cocultivation with Vero cells. SSPE virus-infected Vero cells were maintained in Eagle's minimum essential medium (EMEM) supplemented with 5% calf serum, 0.11% sodium bicarbonate, 100 units of penicillin and 100 pg per ml of streptomycin.…”

Infection of Different Cell Lines of Neural Origin with Subacute Sclerosing Panencephalitis (SSPE) Virus

“…Kobune et al (32) reported that susceptibility of B95a cells (a marmoset B lymphoid cell line transformed by Epstein-Barr virus) to MV in clinical specimens is 10,000 times that of Vero cells. On the other hand, SSPE viruses have generally been isolated from cells of non-lymphoid origin, such as Vero (16,25,35,37), BSC-1 (7), and primary human embryonic lung cells (58). We have tried to isolate our three SSPE viruses by cocultivation of brain cells from patients with SSPE with three different cell lines, Vero, B95a, and primary human embryonic lung cells (44).…”