Neofusicoccum (Botryosphaeriaceae) is a genus of plant pathogenic fungi associated with various woody plants that causes shoot blight, canker, and dieback of fruit and forest trees (Phillips et al., 2008). The genus was established by Crous et al. (2006), based on the type species N. parvum (Pennycook & Samuels) Crous, Slippers & A. J. L. Phillips, and is closely related to the genus Fusicoccum. Neofusicoccum is characterized by fusiform to ellipsoid aseptate conidia and "dichomera-like" synanamorphs, which septate and turn brown with age, to form globose to pyriform conidia (Barber et al., 2005;Phillips, Rumbos, Alves, & Correia, 2005;Crous et al., 2006). The sexual morphs of Botryosphaeriaceae are similar in morphology, such that these structures are difficult to distinguish among the genera Botryosphaeria, Fusicoccum, and Neofusicoccum. These genera have been historically confused. The genus Fusicoccum, which had been regarded as an asexual morph of the genus Botryosphaeria, is known to be polyphyletic and synonymous with various other genera (Phillips, Fonseca, Povoa, Castilho, & Nolasco, 2002;Crous et al., 2006). Therefore, molecular phylogenetic analysis is essential to discriminate between Fusicoccum and other Fusicocum-like genera (Marin-Felix et al., 2017). Taxonomic studies of the genus Neofusicoccum and its related genera have been conducted based on worldwide multi-locus phylogenetic analyses (Dissan-