The column headings of the markers in Table 5 of the original were incorrect. The correct headings are TG231 (left column) and PG3 (right column).The online version of the original article can be found at http:// dx

Resistance to begomoviruses, including bipartite tomato mottle virus (ToMoV) and monopartite tomato yellow leaf curl virus (TYLCV), has been introgressed to cultivated tomato (Solanum lycopersicum) from Solanum chilense accessions LA1932 and LA2779. A major gene, Ty-3, responsible for resistance to ToMoV and TYLCV was previously mapped on the long arm of chromosome 6. In the present study, we identified a 14-cM S. chilense introgression on the long arm of chromosome 3 in some resistant breeding lines derived from LA1932. A new begomovirus resistance locus, Ty-4, was mapped to the 2.3-cM marker interval between C2_At4g17300 and C2_At5g60160 in the introgression. Analysis of a population segregating for Ty-3 and Ty-4 demonstrated that Ty-3 accounted for 59.6% of the variance, while Ty-4 only accounted for 15.7%, suggesting that Ty-4 confers a lesser effect on TYLCV resistance. Recombinant inbred lines (RILs) with Ty-3 and Ty-4 had the highest level of TYLCV resistance. The PCR-based markers tightly linked to the Ty-4 locus as well as the Ty-3 locus have been recently used in our breeding program for efficient selection of high-levels of begomovirus resistance and now allow for efficient breeding by marker-assisted selection.

No abstract

The most widely cultivated species of cotton, Gossypium hirsutum, is a disomic tetraploid (2n=4x=52). It has been proposed previously that extant A- and D-genome species are most closely related to the diploid progenitors of the tetraploid. We used fluorescent in situ hybridization (FISH) to determine the distribution of 5S and 18S-28S rDNA loci in the A-genome species G. herbaceum and G. arboreum, the D-genome species G. raimondii and G. thurberi, and the AD tetraploid G. hirsutum. High signal-to-noise, single-label FISH was used to enumerate rDNA loci, and simultaneous, dual-label FISH was used to determine the syntenic relationships of 5S rDNA loci relative to 18S-28S rDNA loci. These techniques provided greater sensitivity than our previous methods and permitted detection of six new G. hirsutum 18S-28S rDNA loci, bringing the total number of observed loci to 11. Differences in the intensity of the hybridization signal at these loci allowed us to designate them as major, intermediate, or minor 18S-28S loci. Using genomic painting with labeled A-genome DNA, five 18S-28S loci were localized to the G. hirsutum A-subgenome and six to the D-subgenome. Four of the 11 18S-28S rDNA loci in G. hirsutum could not be accounted for in its presumed diploid progenitors, as both A-genome species had three loci and both D-genome species had four. G. hirsutum has two 5S rDNA loci, both of which are syntenic to major 18S-28S rDNA loci. All four of the diploid genomes we examined contained a single 5S locus. In g. herbaceum (A1) and G. thurberi (D1), the 5S locus is syntenic to a major 18S-28S locus, but in G. arboreum (A2) and G. raimondii (D5), the proposed D-genome progenitor of G. hirsutum, the 5S loci are syntenic to minor and intermediate 18S-28S loci, respectively. The multiplicity, variation in size and site number, and lack of additivity between the tetraploid species and its putative diploid ancestors indicate that the behavior of rDNA loci in cotton is nondogmatic, and considerably more complex and dynamic than previously envisioned. The relative variability of 18S-28S rDNA loci versus 5S rDNA loci suggests that the behavior of tandem repeats can differ widely.

A library of ''introgression lines'' containing Solanum lycopersicoides chromosome segments in the genetic background of cultivated tomato (Lycopersicon esculentum) was used to study factors affecting homeologous recombination. Recombination rates were estimated in progeny of 43 heterozygous introgressions and whole-chromosome substitution lines, together representing 11 of the 12 tomato chromosomes. Recombination within homeologous segments was reduced to as little as 0-10% of expected frequencies. Relative recombination rates were positively correlated with the length of introgressed segments on the tomato map. The highest recombination (up to 40-50% of normal) was observed in long introgressions or substitution lines. Double-introgression lines containing two homeologous segments on opposite chromosome arms were synthesized to increase their combined length. Recombination was higher in the double than in the single segment lines, despite a preference for crossovers in the region of homology between segments. A greater increase in homeologous recombination was obtained by crossing the S. lycopersicoides introgression lines to L. pennellii-a phylogenetically intermediate species-or to L. esculentum lines containing single L. pennellii segments on the same chromosome. Recombination rates were highest in regions of overlap between S. lycopersicoides and L. pennellii segments. The potential application of these results to breeding with introgression lines is discussed.

The column headings of the markers in Table 5 of the original were incorrect. The correct headings are TG231 (left column) and PG3 (right column). The online version of the original article can be found at http:// dx.

The most widely cultivated species of cotton, Gossypium hirsutum, is a disomic tetraploid (2n=4x=52). It has been proposed previously that extant A- and D-genome species are most closely related to the diploid progenitors of the tetraploid. We used fluorescent in situ hybridization (FISH) to determine the distribution of 5S and 18S-28S rDNA loci in the A-genome species G. herbaceum and G. arboreum, the D-genome species G. raimondii and G. thurberi, and the AD tetraploid G. hirsutum. High signal-to-noise, single-label FISH was used to enumerate rDNA loci, and simultaneous, dual-label FISH was used to determine the syntenic relationships of 5S rDNA loci relative to 18S-28S rDNA loci. These techniques provided greater sensitivity than our previous methods and permitted detection of six new G. hirsutum 18S-28S rDNA loci, bringing the total number of observed loci to 11. Differences in the intensity of the hybridization signal at these loci allowed us to designate them as major, intermediate, or minor 18S-28S loci. Using genomic painting with labeled A-genome DNA, five 18S-28S loci were localized to the G. hirsutum A-subgenome and six to the D-subgenome. Four of the 11 18S-28S rDNA loci in G. hirsutum could not be accounted for in its presumed diploid progenitors, as both A-genome species had three loci and both D-genome species had four. G. hirsutum has two 5S rDNA loci, both of which are syntenic to major 18S-28S rDNA loci. All four of the diploid genomes we examined contained a single 5S locus. In g. herbaceum (A1) and G. thurberi (D1), the 5S locus is syntenic to a major 18S-28S locus, but in G. arboreum (A2) and G. raimondii (D5), the proposed D-genome progenitor of G. hirsutum, the 5S loci are syntenic to minor and intermediate 18S-28S loci, respectively. The multiplicity, variation in size and site number, and lack of additivity between the tetraploid species and its putative diploid ancestors indicate that the behavior of rDNA loci in cotton is nondogmatic, and considerably more complex and dynamic than previously envisioned. The relative variability of 18S-28S rDNA loci versus 5S rDNA loci suggests that the behavior of tandem repeats can differ widely.

The whitefly-transmitted Tomato yellow leaf curl virus (TYLCV) is a major pathogen of tomatoes grown in tropical and subtropical regions of the world. Several genes of different origins conferring resistance to TYLCV have been introgressed to the cultivated tomato (Solanum lycopersicum), including the single dominant gene, Ty-2, that originated from S. habrochiates and was previously mapped to a 19-cM region on the long arm of chromosome 11 delimited by restriction fragment length polymorphism markers TG36 and TG393. In the present study, we confirmed the dominant inheritance of the Ty-2 gene from TYLCV evaluation and molecular marker analysis of an F2 segregating population derived from a commercial hybrid that carries the Ty-2 gene. Evaluating recombinants recovered from the F2 progeny for TYLCV resistance localized the Ty-2 gene to a marker interval of 5.5 cM between C2_At1g07960 (82.5 cM) and C2_At4g32930 (88 cM). Additional recombinants were identified for the target region carrying the Ty-2 gene. TYLCV evaluation of the progeny from these recombinants further delimited the Ty-2 gene to a 4.5-cM interval between C2_At1g07960 (82.5 cM) and cLEN-11-F24 (87 cM). The smaller introgressions no longer include the fusarium wilt race 2 resistance locus (I-2), which should facilitate combining the two resistance genes in cis configuration. The polymerase chain reaction-based markers developed from the present study can be used to precisely monitor the introgression of the Ty-2 gene, thus offering the opportunity to pyramid TYLCV resistance genes from different sources as well as resistance genes for other pathogens into elite tomato cultivars.