Toward Fine Mapping of the Tomato Yellow Leaf Curl Virus Resistance Gene Ty-2 on Chromosome 11 of Tomato

Ji, Yuanfu; Scott, John W.; Schuster, David J.

doi:10.21273/hortsci.44.3.614

Cited by 53 publications

(47 citation statements)

References 18 publications

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“…The source of begomovirus resistance in BTI-87 was from the inbred line Gc171, which is known to have the Ty-3a and Ty-4 resistance loci on chromosome 6 and chromosome 3, respectively [47,50]. These resistant loci were introgressed from S. chilense LA1932 [43].…”

Section: Methodsmentioning

confidence: 99%

Analysis of wild-species introgressions in tomato inbreds uncovers ancestral origins

et al. 2014

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BackgroundDecades of intensive tomato breeding using wild-species germplasm have resulted in the genomes of domesticated germplasm (Solanum lycopersicum) being intertwined with introgressions from their wild relatives. Comparative analysis of genomes among cultivated tomatoes and wild species that have contributed genetic variation can help identify desirable genes, such as those conferring disease resistance. The ability to identify introgression position, borders, and contents can reveal ancestral origins and facilitate harnessing of wild variation in crop breeding.ResultsHere we present the whole-genome sequences of two tomato inbreds, Gh13 and BTI-87, both carrying the begomovirus resistance locus Ty-3 introgressed from wild tomato species. Introgressions of different sizes on chromosome 6 of Gh13 and BTI-87, both corresponding to the Ty-3 region, were identified as from a source close to the wild species S. chilense. Other introgressions were identified throughout the genomes of the inbreds and showed major differences in the breeding pedigrees of the two lines. Interestingly, additional large introgressions from the close tomato relative S. pimpinellifolium were identified in both lines. Some of the polymorphic regions were attributed to introgressions in the reference Heinz 1706 genome, indicating wild genome sequences in the reference tomato genome.ConclusionsThe methods developed in this work can be used to delineate genome introgressions, and subsequently contribute to development of molecular markers to aid phenotypic selection, fine mapping and discovery of candidate genes for important phenotypes, and for identification of novel variation for tomato improvement. These universal methods can easily be applied to other crop plants.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-014-0287-2) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Analysis of wild-species introgressions in tomato inbreds uncovers ancestral origins

et al. 2014

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“…This marker density has been proven useful to detect wild introgressions associated with resistance in other TYLCV-resistant lines. This is the case of S. habrochaites-derived line H24; the resistance locus Ty-2 was first mapped to a 19 cM region on chromosome 11 using 90 RFLP markers (Hanson et al 2000) and these results were later confirmed by fine mapping this gene to a marker interval of 4.5 cM (Ji et al 2009b). The polymorphic markers used in the present study allowed determining the wild introgressions in each of the resistant lines derived from LA1932, LA1960 and 1971, respectively.…”

Section: Discussionmentioning

confidence: 96%

“…Another TYLCV resistance locus, Ty-4, also derived from LA1932 but conferring a lesser effect on TYLCV resistance, has been mapped on chromosome 3 (Ji et al 2009a). Other resistance loci have been identified from different wild tomato relatives: Ty-2, on chromosome 11 and derived from S. habrochaites (Hanson et al 2006;Ji et al 2009b), and Ty-5, originated from S. peruvianum and mapped on chromosome 4 (Anbinder et al 2009). Recently, a recessive gene from the tomato cultivar Tyking has been reported to be located in the same region as Ty-5 and the symbol ty-5 has been proposed (Hutton et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Genetic control and mapping of Solanum chilense LA1932, LA1960 and LA1971-derived resistance to Tomato yellow leaf curl disease

2012

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Tomato yellow leaf curl disease (TYLCD) is caused by a complex of begomovirus. Breeding for resistance to this disease has mainly been based on Ty-1 gene, derived from Solanum chilense LA1969. Commercial varieties available to date still develop symptoms and suffer yield losses with high inoculum pressure and early infections. It is of interest to incorporate in breeding programs resistance from the different available sources. Lines with resistance to TYLCD derived from S. chilense accessions LA1932, LA1960 and LA1971 were previously developed. The objectives of this work were to study the genetic control of the resistance derived from these accessions and to map the resistance loci. Response to viral infection was assayed in segregating generations derived from these sources. Results obtained were compatible with a monogenic control of resistance. A total of 94 markers were used to locate the S. chilense introgressions in each of the lines. Only the presence of a large introgression in chromosome 6 was common to all lines. Analysis of recombinants allowed localizing the resistance loci in an interval of approximately 25 cM, also common to all five families. This interval includes the region to which two previously S. chilense-derived TYLCD resistance loci have been mapped, the Ty-1/Ty-3 region. This is the first report of LA1960 and LA1971-derived TYLCV resistance loci to be located on chromosome 6. Further work will be done to fine map the loci found in the present work, in order to determine if they are indeed located in the Ty-1/Ty-3 region.

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“…While Ty-2 is a dominant gene, the Ty-3 gene is partially dominant (Hanson et al 2000;Ji et al 2009a;Verlaan et al 2013). The Ty-4 introgression is a minor QTL and explains only 16 % of variation in disease severity.…”

Section: Discussionmentioning

confidence: 99%

“…We used markers previously reported to be linked and located at the opposite ends of Ty-2 and Ty-3 introgressions for performing marker assisted selection (Ji et al 2007a(Ji et al , 2009a (Table S1). In addition, we also used SSR markers reported by Kadirvel et al (2013) to test the possible presence of ty-5 and qTy10.1 in stable Ty-2 and Ty-3 lines developed in this study.…”

Section: Marker Assaysmentioning

confidence: 99%

Marker assisted selection of Ty-2 and Ty-3 carrying tomato lines and their implications in breeding tomato leaf curl disease resistant hybrids

et al. 2015

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Tomato leaf curl virus disease caused by whitefly-transmitted begomoviruses is a serious tomato production constraint in tropical and subtropical regions of the world. Use of genetic resistance for diseases caused by begomoviruses is an important component of sustainable disease management approaches. To date, six introgressions (Ty genes) derived from different tomato wild species that confer resistance to tomato leaf curl disease have been identified. Among these, dominant Ty-2 and partially dominant Ty-3 are important for the development of resistant hybrids because of their gene action. In the present study we report marker assisted selection of tomato lines carrying Ty-2 and Ty-3 genes and the effect of combining these in tomato hybrids using agroinoculation. The response of lines and hybrids with the Ty-2 gene indicated ineffectiveness of Ty-2 to infection by a bipartite tomato leaf curl virus. The Ty-3 gene showed dosage effect with partial resistance in heterozygous state. However, the level of resistance in hybrids with the combination of Ty-2 and Ty-3 genes was higher and similar to homozygous Ty-3 plants.The Ty-2 gene in either homozygous or heterozygous state enhanced the level of resistance of plants with heterozygous Ty-3. Thus, tomato hybrid breeding programs can be benefited by using tomato lines carrying Ty-2 and Ty-3 resistance genes. The combined results of evaluation of horticultural performance, field resistance and agroinoculation tests demonstrated that the set of tomato lines developed in this study could be useful resources for achieving higher level of resistance in tomato hybrids.

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Toward Fine Mapping of the Tomato Yellow Leaf Curl Virus Resistance Gene Ty-2 on Chromosome 11 of Tomato

Cited by 53 publications

References 18 publications

Analysis of wild-species introgressions in tomato inbreds uncovers ancestral origins

Analysis of wild-species introgressions in tomato inbreds uncovers ancestral origins

Genetic control and mapping of Solanum chilense LA1932, LA1960 and LA1971-derived resistance to Tomato yellow leaf curl disease

Marker assisted selection of Ty-2 and Ty-3 carrying tomato lines and their implications in breeding tomato leaf curl disease resistant hybrids

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